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EC number: 604-135-1 | CAS number: 139481-28-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August 19, 2004 - September 29, 2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Name: CAN3
Batch No.: February 2004
Active Ingredient(s) l Purity: > 99% 2-[N-(2'-cianobiphenyl-4-il)-methylanlino]-3-methyhritrobenzoate
Certificate of Analysis: not available
Aggregate State at RT: solid (according to IBACON personnel)
Colour: yellow
Solubility: in water: 0.1 µg/mL
Stability: (i) technical product: see expiry date; (ii) in water: stable under test conditions
Expiry Date: February 2009
Storage: in original container, at room temperature, in the dark - Analytical monitoring:
- no
- Details on sampling:
- - Concentrations: 10, 18, 32, 56 and 100 mg test item/L and a control. In the control, test water was used without addition of the test item.
- Sampling method: The test item was not well soluble in test water. To avoid physical effects of undissolved test item on Daphnia no concentrations above the solubility limit of the test item in test water were tested. Therefore, a stock suspension of 100 mg test item/L was prepared by suspending 80 mg test item in 800 mL test water for preparing the test concentrations. The stock suspension was stirred for 24 hours in the dark to
dissolve as much test item as possible. Then, the undissolved test item was separated by filtration (0.45 µm cellulose nitrate filter) and the required test concentrations were prepared. The test concentrations were prepared just before introduction of the Daphnia (= start ofthe test). - Vehicle:
- yes
- Details on test solutions:
- Measurement of pH, dissolved Oxygen and Water Temperature:
The pH-values and dissolved oxygen concentrations were determined at test start and end in the test media of all test
concentrations and the control. The water temperature was measured in one control beaker at the start and the end of the test.
Behaviour of the Test Item in Test Water:
The behaviour of the test item in test water was determined at the start of the test, after 24 and 48 hours test duration in all test concentrations. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species:
Daphnia magna (Straus), clone 5
Age at Test Start:
6.75 to 22.5 hours old
Sex:
female
Origin:
supplied 1997 by the Umweltbundesamt, Institut fur Wasser-, Boden- und Lufthygiene, Berlin, Germany
Breeding Conditions:
The Daphnia were bred in the laboratories of IBACON under similar temperature and light conditions as in the test, and in
reconstituted water of a similar quality regarding to pH, components of the main ions and total hardness as the test water used in the test. The test organisms were not first brood progeny. The Daphnia of the stock culture were fed at least each working day with green algae (Desmodesmus subspicatus) freshly grown in the laboratories of IBACON.
Toxic Standard:
For the evaluation of the quality of the Daphnia clone and the experimental conditions the substance potassium dichromate
p.A. is tested at least twice a year to demonstrate satisfactory test conditions.
Result of the latest reference test:
48-hour EC 50: 2.12 mg potassium dichromate/L
(confidence limits: 1.02-4.40 mg/L)
Study completion date: February 19, 2004
Acclimatisation:
for 6.75 hours under test conditions. - Test type:
- static
- Water media type:
- other: Reconstituted Water
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- The immobility or mortality of the Daphnia was determined by visual controls after 24 and 48 hours. Those animals not able to swim within 15 seconds after gentle agitation of the test beaker were considered to be immobile.
- Hardness:
- Water Hardness: 2.5 mmol/L (= 250.0 mg/L) as CaCO3
- Test temperature:
- 20-21 °C
- pH:
- 7.7- 8.1
- Dissolved oxygen:
- Oxygen concentrations were determined at test start and end in the test media of all test concentrations and the control. At the end of the test the dissolved oxygen concentration in the test media should be ≥ 2 mg O2/L in the control and test
vessels. - Salinity:
- In deionised water analytical grade salts were added to following nominal concentrations:
CaCl2 x 2H2O 2.0 mmol/L (= 294.0 mg/L)
MgSO4 x 7H2O 0.5 mmol/L (= 123.0 mg/L)
NaHCO3 0.75 mmol/L (= 65.0 mg/L)
KCl 0.075 mmol/L (= 5.8 mg/L)
Water Hardness 2.5 mmol/L (= 250.0 mg/L) as CaCO3
Alkalinity 0.8 mmol/L
Ratio of Ca: Mg = 4 : 1 (based on molarity)
Na : K = 10 : 1 (based on molarity) - Conductivity:
- conductivity < 5 µS/cm
- Nominal and measured concentrations:
- Nominal concentrations: 10, 18, 32,56 and 100 mg test iterm/L
- Details on test conditions:
- Type and Size: glass beakers of 100 mL volume with 80 mL test medium
Identification: Each test unit was uniquely identified with study number, treatment and replicate number.
Test Conditions
Test Environment: controlled environment room
Water Temperature: 20-21 °C
pH-Value of Test Water: 7.7- 8.1
Light Regime: 16 h light : 8 h dark
Light Intensity: 410- 750 lux
Introduction of Individuals: 20 Daphnia per control and test concentration, divided into 4 groups of 5 animals, each group in 80 mL test medium.
Feeding: The test organisms were not fed during the test.
Exposure Time: 48 hours.
Test Procedure: The test item is considered as stable in test water, so a static test system was chosen. - Reference substance (positive control):
- yes
- Remarks:
- For the evaluation of the quality of the Daphnia clone and the experimental conditions the substance potassium dichromate p.A. is tested at least twice a year to demonstrate satisfactory test conditions.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: After 48 hours in the control and at all test concentrations no mortality or immobilization of the test animals was observed.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: After 48 hours in the control and at all test concentrations no mortality or immobilization of the test animals was observed.
- Details on results:
- Validity Criteria of the Study
Control:
The experiment is valid because the mortality of Daphnia magna in the control did not exceed 10 % and no Daphnia were trapped at the water surface.
Dissolved Oxygen Concentration:
At the end of the test the dissolved oxygen concentration in the test media was ≥ 2 mg O2/L in the control and test vessels.
Signs of intoxication after 24 hours:
After 24 hours in the control and at all test concentrations no mortality or immobilization of the test animals was observed.
Signs of intoxication after 48 hours:
After 48 hours in the control and at all test concentrations no mortality or immobilization of the test animals was observed.
pH-Values: 7.7- 8.1
Dissolved Oxygen Concentrations: at least 5.7 mg/L or higher
Water Temperature: 20-21 °C
Behaviour of the Test Item: the test water was coloured by the test item at all test concentrations during the entire study. - Validity criteria fulfilled:
- yes
- Conclusions:
- After 48 hours in the control and at all test concentrations no mortality or immobilization of the test animals was observed.
- Executive summary:
The EC 0, 50 and 100 could not be determined, because there was no effect of the test item at all concentrations. The EC 50 is estimated to be higher than 100 mg test item/L. The 48-hour NOEC of CAN3 to Daphnia magna was determined to be at least nominai 100 mg test item/L. The NOEC might even be higher than this concentration, but concentrations in excess of nominal 100 mg test item/L have not been tested. The 48-hour LOEC were clearly higher than nominal 100 mg test item/L. This value could not be quantified due to the absence of toxicity of CAN3 at all tested concentrations.
Reference
Description of key information
The EC 0, 50 and 100 could not be determined, because there was no effect of the test item at all concentrations. The EC 50 is estimated to be higher than 100 mg test item/L. The 48-hour NOEC of CAN3 to Daphnia magna was determined to be at least nominai 100 mg test item/L. The NOEC might even be higher than this concentration, but concentrations in excess of nominal 100 mg test item/L have not been tested. The 48-hour LOEC were clearly higher than nominal 100 mg test item/L. This value could not be quantified due to the absence of toxicity of CAN3 at all tested concentrations.
Key value for chemical safety assessment
Additional information
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