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EC number: 220-266-3 | CAS number: 2695-37-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- January 4, 1999 to March 30, 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
None specified - Analytical monitoring:
- yes
- Details on sampling:
- 10 ml of test samples were taken from each test group at the start of test and at 72 hours after start and analyzed with HPLC. At the analysis of test solution, standard solution was also measured for each test sample analysis and concentrations of test material were quantitatively determined with peak height of test material.
- Vehicle:
- no
- Details on test solutions:
- 1000 mg/L of mother test solution was prepared by adding sodium p-toluenesulfonate to test medium. This solution was added into medium sterilized with autoclave to prepare test solution for each concentration group.
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Single-cell green algae of Selenastrum capricornutum is used for the test.
This species is aseptically continuous cultured in this laboratory. Growth Inhibition concentration (EbC50) at 72 hours for standard substance (potassium dichromate, special grade reagent) was 0.84 mg/L
Preculture Cultivation
Algae cultured for 4 days before the start of exposure under the same conditions of the test were used for the test. - Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No post exposure observation period specified in the study report.
- Hardness:
- Not specfied in the study report.
- Test temperature:
- Temperature inside algae incubation test equipment was constantly 23.1 °C for 72 hours during exposure period.
- pH:
- pHs of the test solution were 8.3-8.6 at the start of exposure and 9.1- 10.1 at the end of exposure.
- Dissolved oxygen:
- Not specified in the study report.
- Salinity:
- Not specified in the study report.
- Nominal and measured concentrations:
- Nominal concentrations of 1000, 320, 100, 32, 10, 3.2 and 1.0 mg/L of test material groups and control group were set up.
- Details on test conditions:
- Test Method
Test conditions
1) Cultivation method: Shaking cultivation (100 rpm)
2) Temperature: 23±2°C
3) Exposure period: 72 hours
4) Volume of test solution: 100 mL (OECD medium)
5) Lighting: 4000-5000 lux (Continuous lighting)
6) Initial cell concentration: 1 X 104 cells/mL
Growth medium
Growth medium described in OECD Guidelines for the testing chemicals was used in both preliminary cultivation and test.
Test vessels. Algae culturing test equipment and instruments
Test vessels: 300mL Erlenmeyer flask made of glass
Algae culturing test equipment: Algae Test incubator (Miyamoto Riken Kogo GT-40S)
Optical microscope: Olympus inverted incubation microscope (IMT413)
Cell counter: Counting chamber
pH meter: Toa Electronics (HM-30V)
Thermometer: Glass mercury thermometer
Illuminometer: Sibata Scientific Technology Ltd. (AN-F9)
Test procedure
After counting the number of algae cells incubated in the preculture, a certain amount of incubated solution obtained in preliminary cultivation was added into the test vessels containing test solution to adjust cell concentration in the test solution to be 1 X 104 cells/ml.
Each test solution was set up in the incubation equipment at 23 ± 2°C and started test. Cell concentrations were counted at 24, 48 and 72 hours. Cell concentrations were measured by taking 2.0 ml of test solution from each test vessel and counting the number of the cells on the counting chamber using microscope.
pH of the test solutions were measured for 3 replicates and a backup. These value were assigned as pH at the start of exposure. At the end of test, pH for all 3 replicates were measured. During test period, temperature and lighting intensity were measured at least once a day. - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- Environmental factors which might affect reliability of the test result.
None
Concentration of test material in the test solution.
Concentrations of test material at the start of exposure were from 1.0 to 1010 mg/L (nominal concentration from 1.0 to 1000 mg/L). After 72 hours, the concentrations of test material were from 1.0 to 1030 mg/L.
Algae growth curve
Cell concentration of the control increased more than 80 times after 72 hour-incubation. This result showed that cells growth was normal under test conditions.
Fifty percent effective concentration (EC50) and No Observed Effect Concentration (NOEC)
1) Growth inhibition concentration (EbC50) by comparison of area under growth curve.
EbC50 (0-72h) was more than 1000 mg/L. The highest concentration where no significant difference was observed when compared with control (No Observed Effect Concentration (NOECb)) was 10 mg/L.
2) Growth inhibition concentration (ErC50) by comparison of growth rate
ErC50 (24-48 h) and ErC50 (24-72h) were more than 1000 mg/L respectively.
The highest concentration where no significant difference was observed when compared with control (No Observed Effect Concentration (NOECr)) was 1000 mg/L.
Temperature and pH
Temperature inside algae incubation test equipment was constantly 23.1°C for 72 hours during exposure period. pHs of the test solution were 8.3-8.6 at the start of exposure and 9.1- 10.1 at the end of exposure. - Results with reference substance (positive control):
- Reference substance not utilised in this study.
- Reported statistics and error estimates:
- None detailed in the study report.
- Validity criteria fulfilled:
- yes
- Conclusions:
- ErC50 (24- 72h): > 1000 mg/L
No Observed Effect Concentration (NOECr): 1000 mg/L - Executive summary:
Purpose of the study: Growth Inhibition test to algae (Selenastrum capricornutum) of sodium p-toluenesulfonate will be conducted to determine growth inhibition concentration (EC50) and No Observed Effect Concentration (NOEC).
Applied Guideline: This study is done in accordance with OECD Guidelines for the testing chemicals No.201 "Alga, Growth Inhibition test" (1984).
Applied GLP: This study is done in accordance with GLP regulation of Environment Agency.
1) Test substance: Sodium p-toluenesulfonate
2) Incubation: Shaking cultivation (100 rpm)
3) Test organism: Selenastrum capricornutum (ATCC 22662)
4) Temperature: 23±2°C
5) Test duration: 72 hours
6) Volume of test solution: 100mL (OECD medium)
7) Lighting: 4000- 5000 lux (continuous lighting)
8) Initial cell concentration: 1 X 104cells/ml
9) Test Concentration: Control, 1000, 320, 100, 32, 10, 3.2 and 1.0 mg/L
10) Analysis method of test substance in the test solution: HPLC (start and end of the exposure)
Results
1) Growth Inhibition Concentration based on comparison of area under growth curves.
EbC50 (0-72 h): > 1000 mg/L
No Observed Effect Concentration (NOECb): 10mg/L
2) Growth Inhibition Concentration based on comparison of growth rate.
ErC50 (24-48h): > 1000 mg/L
No Observed Effect Concentration (NOECr): 1000 mg/L
ErC50(24- 72h): > 1000 mg/L
No Observed Effect Concentration (NOECr): 1000mg/L
Reference
Measured Concentrations During a 72-Hour Exposure to Selenastrum capricornutum
Nominal Concentration mg/L |
Measured Concentration (mg/L) |
|||
0 Hour mg/L |
Percent of Nominal |
72 Hour mg/L |
Percent of Nominal |
|
1000 |
1010 |
101 |
1030 |
103 |
320 |
327 |
102 |
328 |
103 |
100 |
101 |
101 |
104 |
104 |
32 |
32.9 |
103 |
33.1 |
103 |
10 |
10.1 |
101 |
10.4 |
104 |
3.2 |
3.3 |
103 |
3.4 |
106 |
1.0 |
1.0 |
100 |
1.0 |
100 |
Control |
N.D. |
- |
N.D. |
- |
Cell Density of Selenastrum capricornutum
Nominal Concentration mg/L |
No. |
Cell Density (x104cells/mL) |
|||
0 Hour |
24 Hour |
48 Hour |
72 Hour |
||
Control |
1 2 3 |
1.00 1.00 1.00 |
6.00 6.25 6.50 |
28.25 26.5 27.75 |
94.25 91.75 89.75 |
Average S.D. |
1.0 |
6.25 0.25 |
27.50 0.90 |
91.92 2.25 |
|
1.9 |
1 2 3 |
1.00 1.00 1.00 |
6.75 6.00 5.50 |
28.50 27.75 26.00 |
84.75 81.50 87.75 |
Average S.D. |
1.00 |
6.08 0.63 |
27.42 1.28 |
84.67 3.13 |
|
3.2 |
1 2 3 |
1.00 1.00 1.00 |
6.00 7.00 6.25 |
30.00 26.75 28.75 |
86.00 84.25 85.25 |
Average S.D. |
1.00 |
6.42 0.52 |
28.50 1.64 |
85.17 0.88 |
|
10 |
1 2 3 |
1.00 1.00 1.00 |
5.50 6.25 5.75 |
27.25 28.50 23.50 |
83.00 88.25 87.25 |
Average S.D. |
1.00 |
5.83 0.38 |
26.42 2.60 |
86.17 2.79 |
|
32 |
1 2 3 |
1.00 1.00 1.00 |
6.00 5.25 5.25 |
26.25 23.75 23.00 |
78.50 80.00 85.50 |
Average S.D. |
1.00 |
5.50 0.43 |
24.33 1.70 |
81.33 3.69 |
|
100 |
1 2 3 |
1.00 1.00 1.00 |
5.75 5.25 5.50 |
27.50 25.25 23.25 |
75.50 79.50 77.00 |
Average S.D. |
1.00 |
5.50 0.25 |
25.33 2.13 |
77.33 2.02 |
|
320 |
1 2 3 |
1.00 1.00 1.00 |
4.50 5.25 4.00 |
24.25 24.50 24.50 |
69.50 80.00 71.50 |
Average S.D. |
1.00 |
4.58 0.63 |
24.42 0.14 |
73.67 5.58 |
|
1000 |
1 2 3 |
1.00 1.00 1.00 |
4.50 4.25 4.25 |
22.75 25.75 25.75 |
74.25 72.75 77.00 |
Average S.D. |
1.00 |
4.33 0.14 |
24.75 1.73 |
74.67 2.16 |
Growth Inhibition of Selenastrum capricornutum
Concentration mg/L |
|
Area A(0-72h) |
Inhibition (%) |
Rate |
Inhibition (%) |
Rate |
Inhibition (%) |
IA(0-72h) |
μ (24-48) |
Im (24-48) |
μ (24-) |
Im(24-72) |
|||
Control |
1 |
1893 |
-2.2 |
0.0646 |
-4.7 |
0.0574 |
-2.5 |
2 |
1827 |
1.4 |
0.0602 |
2.4 |
0.0560 |
0.0 |
|
3 |
1839 |
0.8 |
0.0605 |
1.9 |
0.0547 |
2.3 |
|
Average |
1853 |
0.0 |
0.0617 |
0.0 |
0.0560 |
0.0 |
|
1.0 |
1 |
1803 |
2.7 |
0.0600 |
2.8 |
0.0527 |
5.9 |
2 |
1728 |
6.7 |
0.0638 |
-3.4 |
0.0544 |
2.9 |
|
3 |
1749 |
5.6 |
0.0647 |
-4.9 |
0.0577 |
-3.0 |
|
Average |
1760 |
5.0 |
0.0628 |
-1.8 |
0.0549 |
2.0 |
|
3.2 |
1 |
1836 |
0.9 |
0.0671 |
-8.8 |
0.0555 |
0.9 |
2 |
1761 |
5.0 |
0.0559 |
9.4 |
0.0518 |
7.5 |
|
3 |
1803 |
2.7 |
0.0636 |
-3.1 |
0.0544 |
2.9 |
|
Average |
1800 |
2.9 |
0.0622 |
-0.8 |
0.0539 |
3.8 |
|
10 |
1 |
1722 |
7.1 |
0.0667 |
-8.1 |
0.0565 |
-0.9 |
2 |
1833 |
1.1 |
0.0632 |
-2.4 |
0.0552 |
1.4 |
|
3 |
1689 |
8.8 |
0.0587 |
4.9 |
0.0567 |
-1.3 |
|
Average |
1748 |
5.7 |
0.0629 |
-1.9 |
0.0561 |
0.2 |
|
32 |
1 |
1656 |
10.6 |
0.0615 |
0.3 |
0.0536 |
4.3 |
2 |
1596 |
13.9 |
0.0629 |
-1.9 |
0.0567 |
-1.3 |
|
3 |
1644 |
11.3 |
0.0616 |
0.2 |
0.0581 |
-3.8 |
|
Average |
1632 |
11.9 |
0.0620 |
-0.5 |
0.0561 |
-0.2 |
|
100 |
1 |
1644 |
11.3 |
0.0652 |
-5.7 |
0.0536 |
4.3 |
2 |
1626 |
12.2 |
0.0654 |
-6.0 |
0.0566 |
-1.1 |
|
3 |
1554 |
16.1 |
0.0601 |
2.6 |
0.0550 |
1.8 |
|
Average |
1608 |
13.2 |
0.0636 |
-3.1 |
0.0551 |
1.6 |
|
320 |
1 |
1464 |
21.0 |
0.0702 |
-13.8 |
0.0570 |
-1.8 |
2 |
1614 |
12.9 |
0.0642 |
-4.1 |
0.0567 |
-1.3 |
|
3 |
1482 |
20.0 |
0.0755 |
-22.4 |
0.0601 |
-7.3 |
|
Average |
1520 |
18.0 |
0.0700 |
-13.5 |
0.0579 |
-3.4 |
|
1000 |
1 |
1485 |
19.9 |
0.0675 |
-9.4 |
0.0584 |
-4.3 |
2 |
1533 |
17.3 |
0.0751 |
-21.7 |
0.0592 |
-5.7 |
|
3 |
1584 |
14.5 |
0.0751 |
-21.7 |
0.0604 |
-7.9 |
|
Average |
1534 |
17.2 |
0.0725 |
-17.5 |
0.0593 |
-5.9 |
Calculated EC50 and NOEC
Based on IAvalue
|
(mg/L) |
95-Percent Confidence Limits (mg/L) |
EbC50 (0-72h) |
>1000 |
- |
NOECb (0-72h) |
10 |
- |
Based on Imvalue
|
(mg/L) |
95-Percent Confidence Limits (mg/L) |
ErC50 (24-48h) |
>1000 |
- |
NOECr (24-48h) |
1000 |
- |
ErC50 (24-72h) |
>1000 |
- |
NOECr (24-72h) |
1000 |
- |
Temperature In Algal Culture Cabinet
Temperature (°C) |
||||
0h |
24h |
48h |
72h |
Average |
23.1 |
23.1 |
23.1 |
23.1 |
23.1 |
pH Values at 0-Hour and 72-Hour Exposure
Nominal Concentration (mg/L) |
|
pH |
|
0 Hour |
72 Hour |
||
Control |
1 2 3 |
8.6 |
9.4 9.8 9.6 |
1.0 |
1 2 3 |
8.6 |
9.7 10.1 10.0 |
3.2 |
1 2 3 |
8.3 |
9.8 9.6 9.9 |
10 |
1 2 3 |
8.4 |
9.5 9.8 9.6 |
32 |
1 2 3 |
8.4 |
9.8 9.9 9.7 |
100 |
1 2 3 |
8.5 |
9.3 9.7 9.6 |
320 |
1 2 3 |
8.3 |
9.4 9.1 9.6 |
1000 |
1 2 3 |
8.3 |
9.7 9.3 9.5 |
Description of key information
The growth inhibition of algae was determined acording to the OECD 201 test guideline in accordance with GLP, with exposure to Selenastrum capricornutum under static conditions at a test concentration of 1000 mg/l. As the substance is highly water soluble and hydrolytically stable no analytical monitoring was conducted. Following a 72 hour exposure, no immobilisation occured. The ErC50 is >1000 mg/l.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1 000 mg/L
- EC10 or NOEC for freshwater algae:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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