Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 267-032-7 | CAS number: 67762-73-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 23 February 2016 to 25 February 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- -Diet deviated and temperature was only measured during the test period
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 23
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
no data - Analytical monitoring:
- no
- Details on sampling:
- no data
- Vehicle:
- no
- Details on test solutions:
- no data
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain: E201 Daphnia magna
- Source: CEMAS (CEM Analytical Services Ltd).
- Age at study initiation (mean): = 24 hours.
- Feeding during test: The Daphnia were not fed during the test.
- Food type: A suspension of Chlorella vulgaris.
- Frequency: Each working day
CULTURE CONDITIONS
Conditions: Static
Temperature: 20 ± 2°C
Dissolved oxygen: >60% ASV
Photoperiod: 16 hours light and 8 hours dark - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- ± 1 hour
- Hardness:
- No data
- Test temperature:
- The temperature was in the range of 20.3 – 21.2 °C (See attached Material)
- pH:
- The pH was in the range of 6.66 - 6.82
- Dissolved oxygen:
- The dissolved oxygen content was in the range of 6.36-6.72mg/l (See attached Material)
- Salinity:
- The conductivity was recorded as 0.59
- Nominal and measured concentrations:
- Nominal concentrations of 0 and 100mg/l with four replicates
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass containers
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume:40ml
- Aeration: limited
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 reconstituted Daphnia water
- Conductivity: 0.59
OTHER TEST CONDITIONS
- Photoperiod: 16 Hours Light, 8 Hours Dark Cycle
TEST CONCENTRATIONS
- Test concentrations: Nominal: 0, 100mg/l - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Not calculable
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Not calculable
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Not calculable
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Not calculable
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Not calculable
- Duration:
- 24 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Not calculable
- Details on results:
- - Behavioural abnormalities: none
- Mortality of control: none
- Other biological observations: One daphnia trapped in the meniscus of the 100mg/l concentration. However it was still mobile.
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The dissolved concentrations were likely to be lower than stated as EXP1506833 had a 'not known' solubility (estimated to be very low) in water. - Results with reference substance (positive control):
- - Results with reference substance valid
- EC50: 0.6-2.1 mg/l
- Other: done with potassium dichromate - Validity criteria fulfilled:
- yes
- Conclusions:
- The test material showed a relatively low acute toxicity to the selected test species Daphnia Magna with the EC50 being greater than 100 mg/L.
- Executive summary:
The short-term toxicity of the test item to the freshwater invertebrate species Daphnia Magna was examined in accordance to the OECD guideline for the testing of chemicals 202. A water accommodated faction was prepared according to OECD guideline 23 due to the poor solubility of the test item.
A 48 hours toxicity test was performed on the fresh water invertebrate species Daphnia magna acquired from standardized laboratory cultures. The concentrations were nominal during the experiment and the EC50 value was determined by observation of mobility. The tests were conducted under controlled temperature conditions at 20.3 – 21.2 °C with a circadiac light:dark cycle. Dosing was performed under static conditions. The test substance showed low toxicity to the selected species with both the 24 and 48 hour EC50 being >100 mg/L.
Reference
See attached background material.
Description of key information
The 48 hour EC50 of the registered substance was discovered to be greater than 100 mg/L for freshwater invertebrates species Daphnia magna. As 100 mg/L was the highest concentration tested it is proposed to adopt the conservative approach and use this concentration for the purpose of the classification. The registered substance is not considered toxic to invertebrate based on the results of this study.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
The acute toxicity of the registered substance to freshwater invertebrate was determined according to OECD guideline 202 (Daphnia sp. Acute Immobilisation Test). The species used for the study was Daphnia magna (water flea) which was tested under static conditions for 48 hours. The test was carried out under controlled conditions with a temperature of 20.3 -21.2 °C with a light: dark cycle of 16:8 hours. Five organisms were kept in each vessel with four vessels (replicates) used per concentration tested. The concentrations were nominal and the EC50 determined by observation of mobility. The registered substance showed relatively low toxicity to the tested species, with both the 24 and 48 hour EC50 being greater than the highest concentration tested (100 mg/L).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.