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EC number: 202-722-3 | CAS number: 99-03-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data from peer review publication
Data source
Reference
- Reference Type:
- publication
- Title:
- The Induction of Bacterial Mutation and Hepatocyte Unscheduled DNA Synthesis by Mono-substituted Anilines
- Author:
- Christina Z. Thompson, Leo E. Hill, Janet K. Epp, and Gregory S. Probst
- Year:
- 1 983
- Bibliographic source:
- Environmental Mutagenesis 5:803-811 (1983)
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Gradient Plate Test for Bacterial Mutation
- GLP compliance:
- no
- Type of assay:
- bacterial forward mutation assay
Test material
- Reference substance name:
- 3'-aminoacetophenone
- EC Number:
- 202-722-3
- EC Name:
- 3'-aminoacetophenone
- Cas Number:
- 99-03-6
- Molecular formula:
- C8H9NO
- IUPAC Name:
- 1-(3-aminophenyl)ethan-1-one
- Details on test material:
- Name of test material (as cited in study report): m-aminoacetophenone
Substance type: Organic
Physical state: Liquid
Purchased from: Aldrich Chemical Company,
Inc. (Milwaukee, WI) or from Eastman Chemical Company (Rochester, NY).
Constituent 1
Method
- Target gene:
- Plate Incorporation
S typhimurium (G46, TA 1535, TA 100, C3076,
TA1537, D3052, TA1538, and TA98) and two tryptophan auxotrophs of E coli (WP2
and WF2uvrA-).
Species / strain
- Species / strain / cell type:
- other: S typhimurium (G46, TA 1535, TA 100, C3076, TA1537, D3052, TA1538, and TA98) and two tryptophan auxotrophs of E coli (WP2 and WF2uvrA-).
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Test concentrations with justification for top dose:
- 0-1000 µg/plate
- Vehicle / solvent:
- Vehicle used: DMSO
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: Streptozotocin (STZ) (Sigma Chemical Company, St. Louis, MO) and 2-acetylaminofluorene (2-AAF) (Aldrich Chemical Company, Milwaukee, WI) served as positive controls for the bacterial mutation assay.
- Details on test system and experimental conditions:
- METHOD OF APPLICATION :
in agar (plate incorporation)
DURATION
No data
SELECTION AGENT (mutation assays):No data
SPINDLE INHIBITOR (cytogenetic assays):No data
STAIN (for cytogenetic assays):No data
NUMBER OF REPLICATIONS: No data
NUMBER OF CELLS EVALUATED:
No data - Evaluation criteria:
- The results indicate the number of strains
showing chemically induced revertant colonies as well as the concentration range
over which mutation was observed in the most sensitive strain.
Results and discussion
Test results
- Species / strain:
- other: S typhimurium (G46, TA 1535, TA 100, C3076, TA1537, D3052, TA1538, and TA98) and two tryptophan auxotrophs of E coli (WP2 and WF2uvrA-).
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not valid
- Positive controls validity:
- valid
- Additional information on results:
- No data
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive Positive with and without metabolic activation in S typhimurium TA100, TA98,TA1538
Mono- substituted aniline derivatives were tested for the induction of bacterial mutation using eight S typhimurium and two E coli strains. The gradient plate test for bacterial mutation was conducted with and without metabolic activation.
m-amino acetophenone exhibited mutagenic activity with and without metabolic activation in S typhimurium TA100, TA98,TA1538 strains
The Mutagenic concentration rang (MCR) in µg/ml of agar for the positive strains is 10-1000. - Executive summary:
Mono- substituted aniline derivatives were tested for the induction of bacterial mutation using eight S typhimurium and two E coli strains. The gradient plate test for bacterial mutation was conducted with and without metabolic activation.All compounds were tested as supplied without further purification or attempt to identify impurities. Streptozotocin (STZ) (Sigma Chemical Company,St.Louis, MO) and 2-acetylaminofluorene (2-AAF) (Aldrich Chemical Company, Milwaukee, WI) served as positive controls for the bacterial mutation assay.
This test employed ten bacterial strains: eight histidine auxotrophs of S typhimurium (G46, TA 1535, TA100,C3076, TA1537, D3052, TA1538, and TA98) and two tryptophan auxotrophs of E coli (WP2 and WF2uvrA-). Each compound was incorporated into the top wedge of agar on each of four square plates to give a 10-fold concentration gradient per plate and thus provide a 10,000-fold concentration range for the compound. All tests were conducted with and without Aroclor 1254-induced rat liver S-9 and a maximum compound concentration of 1000 µg/ml of agar.
The results indicate the number of strains showing chemically induced revertant colonies as well as the concentration range over which mutation was observed in the most sensitive strain.
m-amino acetophenone exhibited mutagenic activity with and without metabolic activation in S typhimurium TA100, TA98,TA1538 strains
The Mutagenic concentration rang (MCR) in µg/ml of agar for the positive strains is 10-1000
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