Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 289-904-6 | CAS number: 90045-43-5 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Citrus paradisi M., Rutaceae.
Endpoint summary
Administrative data
Description of key information
For Grapefruit oil, no valid studies on the aquatic toxicity are available for daphnia, fish and algae. For these endpoints, read-across is conducted from the citrus oils (orange oil, mandarin oil, lime oil). The justification of this read-across is described in detail in the read-across justification document.
Daphnia
Valid WAF studies are available for orange oil, mandarin oil and lime oil. The highest toxicity (lowest WAF values) was observed in the studies with orange oil (Vinken and Wydra 2007, Rel. 2):
The study was carried out in closed glass containers and during the preparation of the test media, precautions were taken related to the volatility of the test substance. The highest concentrations were prepared according to the WAF procedure, whereas the concentrations below 1 mg/l were diluted from the WAF of 1 mg/l. However, the effects were observed in the range of the true WAF.
The values of the other citrus oils are used as supporting studies.
Algae
Key WAF studies are available for orange oil, mandarin oil and lime oil. The studies are conducted according to OECD 201 and are validated with reliability 1 or 2. The WAF toxicity values (ErL50) for mandarin oil and lime oil are very similar, while for orange oil the WAF result showed an ErL50 10 times higher (less toxic). The study with lime oil distilled constitutes the lowest toxicity value (lowest WAF value, highest toxicity) for this category and is selected as key study. The values for orange oil, mandarin oil and the other quality of lime oil are used as supporting studies.
Fish
For fish key WAF studies are available for Orange oil, Mandarin oil and Lime oil. The study for orange oil constitutes the lowest toxicity value (lowest WAF value, highest toxicity) for this category and is selected as key study.
An overview of all results with Reliability 1 or 2 is presented in the table below:
Test |
Substance and Reference |
Result (nominal, WAF tests) |
Daphnia OECD 202 KEY STUDY |
Orange oil, IBACON 2007 |
WAF: 48h-EL50 = 1.1 mg/L 48h-NOEL = 0.48 mg/L |
Daphnia OECD 202 Supporting study
|
Mandarin oil, Harlan 2011 |
WAF: 48h-EL50 = 8.9 mg/L 48h-NOEL = 2.2 mg/L
|
Daphnia OECD 202 Supporting study
|
Lime oil distilled, Harlan 2011 |
WAF: 48h-EL50 = 5.0 mg/L 48h-NOEL = 2.2 mg/L
|
Daphnia OECD 202 Supporting study
|
Lime oil CP, Harlan 2011 |
WAF: 48h-EL50 = 12 mg/L 48h-NOEL = 4.8 mg/L
|
Algae OECD 201 Supporting study
|
Mandarin oil, Harlan 2011 |
72h-NOELr = 3.2 mg/L 72h-ErL50 = 9.7 mg/L |
Algae OECD 201 Supporting study |
Orange oil, Fresenius 05.12.2007 |
72h-NOELr = 50 mg/L 72h-ErL50 ~ 150 mg/L |
Algae OECD 201 KEY study |
Lime oil distilled, Harlan 2011 |
72h-NOELr = 3.2 mg/L 72h-ErL50 = 8 mg/L
|
Algae OECD 201 Supporting study |
Lime oil CP, Harlan 2011 |
72h-NOELr = 3.2 mg/L 72h-ErL50 = 11 mg/L |
Fish OECD 203 KEY STUDY |
Orange oil SGS Fresenius 2010 |
96h-NOEL: 4.0 mg/l 96h-LL50: 5.65 mg/l |
Fish OECD 203 Supporting study |
Lime oil, Harlan 2011 |
96h-NOEL: 18 mg/l 96h-LL50: > 18 mg/l |
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
