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EC number: 219-581-9 | CAS number: 2467-13-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
Exposure Pseudokirchneriella subcapitata to an analogue test item gave a 72-Hour EC50 value based on growth rate of 9.0 mg/L (95% CI = 7.8 -11 mg/L). The No Effect Concentration was 1.9 mg/L and the LOEC was considered to be 5.1 mg/L (OECD 201 and EU Method C.3).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 9 mg/L
- EC10 or NOEC for freshwater algae:
- 1.9 mg/L
Additional information
Introduction
A study was performed to assess the effect of the analogue test item on the growth of the green alga Pseudokirchneriella subcapitata. The method followed that described in the OECD Guidelines for Testing of Chemicals (2006) No 201, "Freshwater Alga and Cyanobacteria, Growth Inhibition Test" referenced as Method C.3 of Commission Regulation (EC) No 761/2009.
Methods
Due to the low aqueous solubility and pure nature of the test item, for the purposes of the definitive test, the test medium was prepared as a slow stir saturated solution. Following preliminary range-finding tests, Pseudokirchneriella subcapitata was exposed to solutions of the test item at nominal concentrations of 1.0, 3.2, 10, 32 and 100% v/v saturated solution (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 ± 1 °C. The test item solutions were prepared by stirring an excess (100 mg/L) of test item in culture medium using a magnetic stirrer at a rate such that a dimple was formed at the water surface for 23 hours. After the stirring period, the saturated solution was allowed to stand for 1-Hour prior to the removal of any undissolved test item by filtration through a glass wool plug (first approximate 75-100 mL discarded) to produce a 100% v/v saturated solution of the test item. This saturated solution was then further diluted as necessary, to provide the remaining test groups. Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a Coulter® Multisizer Particle Counter.
Results
Chemical analysis of the test preparations at 0 and 72 hours showed measured boron concentrations to range from 0.070 to 2.5 mg/L. Total Organic Carbon (TOC) analysis of test preparations at 0 and 72 hours showed measured carbon concentrations to range from less than the limit of quantification (LOQ), determined to be 1.0 mg C/L at 1.0% v/v saturated solution, through to 32 mg/L at 100% v/v saturated solution (equivalent to test item concentrations of less than the LOQ to 42 mg/L based on a test item carbon content of 75.78%). It was considered appropriate to calculate the results based on the equivalent test item concentrations obtained from TOC analysis as these results give an estimate of the total dissolved test item concentrations present.
Exposure of the test item to Pseudokirchneriella subcapitata gave a 72-Hour EC50 value based on growth rate of 9.0 mg/L (95% CI = 7.8 -11 mg/L). The No Effect Concentration was 1.9 mg/L and the LOEC was considered to be 5.1 mg/L.
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