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EC number: 943-381-7 | CAS number: 2156594-85-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014-12-17 to 2014-12-19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study, no deficiencies
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Fatty acids, C18-unsatd., compd. with 2-propanol, 1,1’-iminobis-, N-C12-18-(even numbered) alkyl derivs.
- Cas Number:
- 2156594-85-1
- Molecular formula:
- Not applicable
- IUPAC Name:
- Fatty acids, C18-unsatd., compd. with 2-propanol, 1,1’-iminobis-, N-C12-18-(even numbered) alkyl derivs.
- Test material form:
- other: viscous liquid
- Details on test material:
- - Name of test material (as cited in study report): Fatty acids, C18-unsatd., compd. with 2-propanol, 1,1’-iminobis-, N-C12-18-(even numbered) alkyl derivs.
- Physical state: Brown , slightly turbid, viscous liquid
Storage condition of test material: Room temperature, protected from light, in original container
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- All concentration levels and the control were analytically verified by LC-MS/MS at the start of both exposure intervals (0 and 24 h) and at the end of both exposure intervals (24 and 48 h).
At the start of the exposure intervals (0 and 24 h), sampling was carried out after preparation of the test concentrations.
At the end of the exposure intervals (24 and 48 h), samples were taken directly from the test vessels. Adsorption was determined as specified below. The method was validated prior to this study according to SANCO 3029/99 rev.4 (2000-07-11).
Recoveries of the test item should be within ± 20 % of the nominal or initially measured concentrations.
However, the effect levels given are based on the nominal concentrations of the test item Fatty acids, C18-unsatd., compd. with 2-propanol, 1,1’-iminobis-, N-C12-18-(even numbered) alkyl derivs.
Determination of losses due The sorption of the test item on glass was quantified at the
to adsorption onto glassware concentration levels of 1.00 – 2.00 – 4.00 mg/L (double determinations) at the end of both exposure intervals (24 and 48 h). Two additional replicates per concentration level without daphnids were prepared. After sampling for the test medium analysis (aqueous phase), the glassware was emptied and an appropriate amount of a suitable solvent was added. The concentration of the test item in this solution was measured and the adsorbed test item amount was calculated from this concentration. Before this procedure, the test vessels were carefully rinsed with demineralised water to remove the remaining test media.
Schedule of Specific Test Item Analysis
Sampling Date Samples intended to be measured:
[h]
0 and 24 h
(fresh media) aqueous phase
without daphnids:
all test media and control
24 and 48 h
(old media) aqueous phase
with daphnids:
all test media and control
aqueous phase and glass
surface without daphnids:
1.00 – 2.00 – 4.00 mg/L
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The stock solution (8.00 mg/L of the test item were weighed out) was freshly prepared with dilution water prior to each exposure interval (0 and 24 h)
The stock solution was treated with ultrasound for 15 minutes at 40 °C and stirred thereafter with approximately 1100 rpm for 30 minutes at room temperature.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Clone 5
- Source:
Origin: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu)
Breeder: DR.U.NOACK-LABORATORIEN, Käthe-Paulus-Str. 1, 31157 Sarstedt, Germany
- Age at study initiation (mean and range, SD): 2 to 24 h old daphnids from a healthy stock were used for the study
- Method of breeding:
Culture: In glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20 +- 2°C, in an incubator, 16 h illumination; light intensity
of max. 20 µE x m-2 x s-1
Culture medium: Elendt M4, according to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaCO3/L
Cultur feeding: At least 5 times per week ad libitum with a mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata and
Desmodesmus subspicatus, with an algae cell density of > 106 cells/mL
- Feeding during test: No feeding
ACCLIMATION
- Acclimation period: At least 2 h in dilution water.
- Acclimation conditions (same as test or not): Same as test conditions.
- Health during acclimation (any mortality observed): No mortality observed.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Dilution Water Total Hardness
Dated: [mg CaCO3/L]
0 h: 255
2014-12-17
24 h: 262
2014-12-18 - Test temperature:
- 18 - 22°C, constant within ± 1°C
- pH:
- pH at the Start of the Exposure Intervals (0 and 24 h)
(measured in one additional replicate per concentration level and control)
Nominal
test item
concentration
[mg/L] 0 h 24 h
pH-value pH-value
8.00 7.60 7.60
4.00 7.62 7.62
2.00 7.66 7.67
1.00 7.68 7.69
0.500 7.70 7.69
0.250 7.73 7.71
Control 7.61 7.64
pH at the End of the 1st Exposure Interval (24 h)
(measured in all replicates)
Nominal
test item
concentration
[mg/L] pH-values
Replicates
1 2 3 4
8.00 7.48 7.47 7.47 7.45
4.00 7.48 7.46 7.45 7.47
2.00 7.47 7.47 7.46 7.46
1.00 7.48 7.47 7.47 7.48
0.500 7.45 7.44 7.49 7.47
0.250 7.49 7.47 7.48 7.45
Control 7.60 7.53 7.49 7.48
pH at the End of the 2nd Exposure Interval (48h)
(measured in all replicates)
Nominal
test item
concentration
[mg/L] pH-values
Replicates
1 2 3 4
8.00 7.55 7.57 7.57 7.56
4.00 7.58 7.56 7.56 7.56
2.00 7.58 7.57 7.56 7.59
1.00 7.59 7.59 7.57 7.58
0.500 7.62 7.61 7.61 7.56
0.250 7.66 7.64 7.63 7.63
Control 7.67 7.69 7.68 7.67 - Dissolved oxygen:
- O2-concentration at the Start of the Exposure Intervals (0 and 24 h)
(measured in one additional replicate per concentration level and control)
Nominal
test item
concentration
[mg/L] 0 h 24 h
Dissolved Dissolved
O2-concentration [mg/L] O2-concentration [mg/L]
8.00 8.14 7.76
4.00 8.67 8.40
2.00 8.92 8.72
1.00 9.02 8.88
0.500 9.07 8.95
0.250 9.08 8.95
Control 9.17 8.90
O2-concentration at the End of the 1st Exposure (24 h)
(measured in all replicates)
Nominal
test item
concentration
[mg/L] Dissolved O2 -concentration [mg/L]
Replicates
1 2 3 4
8.00 8.32 8.50 8.38 8.40
4.00 8.54 8.38 8.31 8.35
2.00 8.65 8.33 8.43 8.49
1.00 8.49 8.51 8.53 8.50
0.500 8.65 8.63 8.55 8.62
0.250 8.68 8.66 8.68 8.57
Control 8.67 8.57 8.50 8.52
O2-concentration at the End of the 2nd Exposure (48 h)
(measured in all replicates)
Nominal
test item
concentration
[mg/L] Dissolved O2 -concentration [mg/L]
Replicates
1 2 3 4
8.00 8.48 8.64 8.68 8.25
4.00 8.33 8.52 8.30 8.51
2.00 8.60 8.60 8.56 8.62
1.00 8.62 8.45 8.58 8.68
0.500 8.71 8.49 8.49 8.65
0.250 8.72 8.73 8.74 8.79
Control 8.73 8.56 8.68 8.72 - Nominal and measured concentrations:
- 6 test item concentrations in a geometric series with a separation factor of 2, prepared by diluting the stock solution of 8.00 mg/L with dilution water, were tested as follows:
0.250 - 0.500 - 1.00 - 2.00 - 4.00 - 8.00 mg/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (4 cm ID x 7 cm H), 50 mL capacity, loosely covered with watch glasses
- Test volume: 20 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO Test water, according to OECD 202, Annex 3
Component Concentration [mg/L]
KCl 5.76
NaHCO3 64.8
CaCl2 x 2 H2O 294
MgSO4 x 7 H2O 123
- Water Quality Parameters of the Dilution Water prior to the Start of the Exposure Intervals (0 and 24 h):
Dilution Water
Dated: pH-Value Dissolved O2-Concentration [mg/L] Temperature [°C] Conductivity [µS/cm] Total Hardness [mg CaCO3/L]
0 h:2014-12-17 7.61 9.17 19.5 639 255
24 h:2014-12-18 7.64 8.90 20.9 643 262
- Culture medium different from test medium: Elendt M4, according to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaCO3/L
- Intervals of water quality measurement:interval start (0 and 24 h) and interval end (24 and 48h)
OTHER TEST CONDITIONS
- Photoperiod: 16/8 h light/dark cycle
- Light intensity: Diffuse light, light intensity of max. 20 µE x m-2 x s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Immobilisation, 24 h interval
TEST CONCENTRATIONS
- Test concentrations: 0.250 - 0.500 -1.00 - 2.00 - 4.00 - 8.00 mg/L (spacing factor 2 )
- Range finding study:
A non GLP preliminary range finding test under semi-static conditions over a period of 48 hours was conducted at the test facility with three nominal test item concentrations of 0.100, 1.00 and 10.0 mg/L. In the range finding test, two replicates per treatment group and control, each with ten daphnids, were tested. The biologically results are presented in the table below. The results of the analytical monitoring during the range finding test are presented in the tables below
Immobilisation Rates in the non GLP Preliminary Range Finding Test
(n = 20, divided into 2 replicates with 10 daphnids each)
Nominal test item concentration
[mg/L] IMMOBILISATION [%]
24 h 48 h
Replicates Replicates
1 2 MV 1 2 MV
10.0 90 100 95 100 100 100
1.00 0 0 0 0 0 0
0.100 0 0 0 0 0 0
Control 0 0 0 0 0 0
MV = mean value
During the range finding test, the test solutions were clear. The exposure concentrations were monitored at the start (0 and 24 h) and at the end (24 and 48 h) of the exposure intervals in test vessel with daphnids and test vessels without daphnids.
Measured Concentrations of the Component Test Item during the non GLP Preliminary Range Finding Test
Sampling date 2014-11-19 2014-11-20 2014-11-20 2014-11-21
0 h 24 h 24 h 48 h
Start of the End of the Start of the End of the
exposure interval exposure interval exposure interval exposure interval
Nominal
test item
concentration
[mg/L] Fatty acids, C18-unsatd., compd. with 2-propanol, 1,1’-iminobis-, N-C12-18-(even numbered) alkyl derivs.
Meas. Meas. Meas. Meas.
conc. conc. conc. conc.
[mg/L] % [mg/L] % [mg/L] % [mg/L] %
10.0 10.2 102 7.06 71 8.02 80 7.94 79
1.00 1.11 111 0.869 87 1.04 104 1.06 106
0.100 0.118 118 0.0827 83 0.104 104 0.0792 79
Control < 0.0100 < 0.0100 < 0.0100 < 0.0100
Meas. conc. = measured concentration, mean value of 2 injections, dilution factor taken into account
% = percent of the nominal concentration of the test item
The sorption of the test item on glass was quantified at the end of both exposure intervals (24 and 48 h). For this purpose, an additional replicate per concentration level without daphnids was prepared. After sampling for the test medium analysis (aqueous phase), the glassware was emptied and an appropriate amount of a suitable solvent was added. The concentration of the test item in this solution was measured and the adsorbed test item amount was calculated from this concentration. Before this procedure, the test vessels were carefully rinsed with demineralised water to remove the remaining test media.
Measured Concentrations of the Test Item in Aqueous Phase and at Glass Walls of Test Vessels without Daphnids during the non GLP Preliminary Range Finding Test
Fatty acids, C18-unsatd., compd. with 2-propanol, 1,1’-iminobis-,
N-C12-18-(even numbered) alkyl derivs.
Nominal End of the 1st exposure interval End of 2nd exposure interval
test item (24 h) (48 h)
concentration Aqueous Phase Adsorbed Aqueous Phase Adsorbed
[mg/L] 20 mL to Glass 20 mL to Glass
Meas. Meas. Meas. Meas
conc. conc.* conc. conc.*
[mg/L] % [mg/L] % [mg/L] % [mg/L] %
10.0 7.78 78 1.02 10 7.95 80 0.823 8
1.00 0.905 91 0.0406 4 0.885 89 0.0314 3
0.100 0.0828 83 < 0.0100 0.0606 61 < 0.0100
Meas. conc. = measured concentration, mean value of 2 injections, dilution factor taken into account
% = percent of the nominal concentration of the test item - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2.63 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 2.36 - 2.91
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.46 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 4.26 - 4.70
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- See below
- Results with reference substance (positive control):
- The percentage immobility for the reference item was determined after 24 h. The EC50-value with 95 % confidence limits (Cl) was calculated by sigmoidal dose-response regression. The EC50-value for the most recent of the monthly performed reference tests was:
EC50: 2.05 mg/L (Cl 1.90 - 2.18 mg/L)
The EC50-value of the reference item potassium dichromate after 24 h is within the prescribed concentration range of 0.6 - 2.4 mg/L of quality criteria according to AQS P 9/2 (02/2000) for daphnids clone 5 cultured in Elendt M4 medium. The EC50-value of the reference item is also within the recommended range of 0.6 - 2.1 mg/L according to OECD-Guideline 202.
Any other information on results incl. tables
Biological Data
The percentage immobility, determined in all test and control groups after 24 and 48 h under semi-static conditions, is given in the table below.
Immobilisation Rates after 24 and 48 h of Exposure in the Definitive Test
(n = 20, divided into 4 replicates with 5 daphnids each)
Nominal test item concentrations
[mg/L] |
IMMOBILISATION [%] |
|||||||||
24 h |
48 h |
|||||||||
Replicates |
Replicates |
|||||||||
1. |
2. |
3. |
4. |
MV |
1. |
2. |
3. |
4. |
MV |
|
8.00 |
20 |
0 |
20 |
0 |
10 |
100 |
80 |
80 |
100 |
90 |
4.00 |
0 |
0 |
0 |
0 |
0 |
40 |
40 |
40 |
40 |
40 |
2.00 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1.00 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.500 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.250 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
MV = mean value
All test concentrations were visually clear throughout the exposure period.
Measured Exposure Concentrations during the Definitive Test
The concentrations of the test itemwereanalytically verified via LC-MS/MS at the start of the exposure intervals (0 and 24 h) and at the end of the exposure intervals (24 and 48 h) in all concentration levels and the control.
The measured concentrations of the test item at the start of the exposure intervals (0 and 24 h) were in the range of 78 to 112 % of the nominal values. At the end of the exposure intervals (24 and 48 h), the measured concentrations of the test item were in the range of 72 to 90 % of the nominal values.The analytical results arepresented in the table below.
Adsorption to the glassware was found negligible. However, the measured concentrations were successfully maintained for the duration of the test at the biologically relevant test item concentrations.
Measured Concentrations of theTest ItemFatty acids, C18-unsatd., compd.with 2-propanol, 1,1’-iminobis-, N-C12-18-(even numbered) alkyl derivs.during the Definitive Test
Sampling date |
2014-12-17 Start of the exposure interval 0 h |
2014-12-18 End of the exposure interval 24 h |
2014-12-18 Start of the exposure interval 24 h |
2014-12-19 End of the exposure interval 48 h |
||||
Start of analysis |
2014-12-17 |
2014-12-18 |
2014-12-18 |
2014-12-19 |
||||
Nominal test item concentration [mg/L] |
Fattyacids, C18-unsatd., compd. with 2-propanol, 1,1’-iminobis-, N-C12-18-(even numbered) alkyl derivs. |
|||||||
Meas. conc. [mg/L] |
% |
Meas. conc. [mg/L] |
% |
Meas. conc. [mg/L] |
% |
Meas. conc. [mg/L] |
% |
|
8.00 |
6.591) |
82 |
6.82 |
85 |
8.53 |
107 |
7.09 |
89 |
4.00 |
3.411) |
85 |
3.24 |
81 |
4.48 |
112 |
3.55 |
89 |
2.00 |
1.551) |
78 |
1.432) |
72 |
2.09 |
105 |
1.69 |
85 |
1.00 |
0.7901) |
79 |
0.7222) |
72 |
1.033) |
103 |
0.896 |
90 |
0.500 |
0.4081) |
82 |
0.402 |
80 |
0.551 |
110 |
0.398 |
80 |
0.250 |
0.2071) |
83 |
0.1842) |
74 |
0.261 |
104 |
0.1842) |
74 |
Control |
< LOQ |
< LOQ |
< LOQ |
< LOQ |
Meas. conc.= measured concentration of the test item, mean value of 2 injections, dilution factor taken into account
% = percent of the nominal concentration of the test item
LOQ = limit of quantification (20.0 µg/L of the test item)
1) = reanalysis on 2014-12-19, mean value of 2 replicates with 2 injections each, dilution factor taken into account
2) = reanalysis on 2014-12-19, mean value of 3 replicates with 2 injections each, dilution factor taken into account
3) = reinjected on 2014-12-18
Adsorption to Glassduring the Definitive Test
Adsorption to the glass walls of the test vessels was determined exemplarily at the concentration levels of 1.00 – 2.00 – 4.00 mg/L (double determinations) in separate replicates without daphnids at the end of both exposure intervals (24 and 48 h).
Two additional replicates per concentration level without daphnids were prepared. After sampling for the test medium analysis (aqueous phase), the glassware was emptied, rinsed carefully with demineralised water and an appropriate amount of a suitable solvent was added. The concentration of the test item in this solution was measured and the adsorbed test item amount was calculated from this concentration.
Adsorption to the glass walls was in the range of 3 – 7 % of the nominal test item concentrations. For details, see the table below
Fatty acids, C18-unsatd., compd.with 2-propanol, 1,1’-iminobis-, N-C12-18-(even numbered) alkyl derivs. |
||||||||
|
Aqueous Phase 20 mL |
Adsorbed to Glass |
Aqueous Phase 20 mL |
Adsorbed to Glass |
||||
End of the 1stexposure interval |
after exposure of 24 hours
|
End of the 2ndexposure interval |
after exposure of 24 hours
|
|||||
Meas. conc. [mg/L] |
% |
Meas. conc.* [mg/L] |
% |
Meas. conc. [mg/L] |
% |
Meas. conc.* [mg/L] |
% |
|
4.00 |
3.29 |
82 |
0.154 |
4 |
3.73 |
93 |
0.267 |
7 |
2.00 |
1.69 |
85 |
0.0800 |
4 |
1.88 |
94 |
0.102 |
5 |
1.00 |
0.802 |
80 |
0.0273 |
3 |
0.860 |
86 |
0.0353 |
4 |
Meas. conc.= measured concentration of the test item, mean value of 2 injections, dilution factor taken into account
% = percent of the nominal concentration of the test item
LOQ = limit of quantification (20.0 µg/L of the test item)
* = mean value of 2 replicates with 2 injections each
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the nominal concentrations of the test item Fatty acids, C18-unsatd., compd. with 2-propanol, 1,1’-iminobis-, N-C12-18-(even numbered) alkyl derivs., the 48 h-EC50 for Daphnia magna was 4.46 mg/L (95 % confidence limits: 4.26 – 4.70 mg/L).
The NOEC after 48 h was 2.00 mg/L. The LOEC after 48 h was 4.00 mg/L. - Executive summary:
In the acute immobilisation test withDaphnia magna (STRAUS), the effects of the test item Fatty acids, C18-unsatd., compd.with 2-propanol, 1,1’-iminobis-, N-C12-18-(even numbered) alkyl derivs.(batch number:DEG4273438) were determined according to OECD 202 (2004) from2014-12-17 to 2014-12-19 at Dr.U.Noack-Laboratorien, Käthe-Paulus-Str.1, 31157 Sarstedt, Germany.
The study was conducted under semi-static conditions over a period of 48 h with6concentrations of the test itemFatty acids, C18-unsatd., compd.with 2-propanol, 1,1’-iminobis-, N-C12-18-(even numbered) alkyl derivs.in the range of 0.250 to 8.00 mg/Lin a geometric series with a separation factor of 2.
The test item is a mixture of salt and ester of primary fatty amine propoxylate and fatty acid and forms an emulsion in water up to a maximum of 181 mg/L (20 °C). The test item is a brown, slightly turbid and viscous liquid.All test concentrations were visually clear throughout the exposure period.
Twenty daphnids were exposed to each concentration leveland the control.
The concentrations of the test itemwereanalytically verified via LC-MS/MS at the start of the exposure intervals (0 and 24 h) and at the end of the exposure intervals (24 and 48 h) in all concentration levels and the control.
The measured concentrations of the test item at the start of the exposure intervals (0 and 24 h) were in the range of 78 to 112 % of the nominal values. At the end of the exposure intervals (24 and 48 h), the measured concentrations of the test item were in the range of 72 to 90 % of the nominal values..
Adsorption to the glass walls of the test vessels was determined exemplarily at the concentration levels of 1.00 – 2.00 – 4.00 mg/L (double determinations) in separate replicates without daphnids at the end of both exposure intervals (24 and 48 h).
Adsorption to the glass walls was in the range of 3 – 7 % of the nominal test item concentrations. .Adsorption to the glassware was found negligible. However, the measured concentrations were successfully maintained for the duration of the test at the biologically relevant test item concentrations. The effect levels given are based on the nominal concentrations of the test itemFatty acids, C18-unsatd., compd.with 2-propanol, 1,1’-iminobis-, N-C12-18-(even numbered) alkyl derivs.
The water quality parameters (i.e. pH-value and dissolved oxygen concentration), measured at the start (0 and 24 h) and at the end of the exposure intervals (24 and 48 h), were within the acceptable limits. The validity criteria of the test guideline were fulfilled.
Based onthe nominal concentrationsof the test itemFatty acids, C18-unsatd., compd.with 2-propanol, 1,1’-iminobis-, N-C12-18-(even numbered) alkyl derivs.,
the 48 h-EC50forDaphnia magnawas 4.46 mg/L (95 % confidence limits: 4.26 – 4.70 mg/L).
The NOEC after 48 h was 2.00 mg/L.The LOEC after 48 h was 4.00 mg/L.
EC10-, EC50- (with Confidence Interval), EC100-Values, NOEC and LOEC
(based on the nominal concentrations of the test item)
Fattyacids, C18-unsatd., compd. with 2-propanol, 1,1’-iminobis-, N-C12-18-(even numbered) alkyl derivs.
Effect levels
Test duration
[h]
Nominal test item concentrations
[mg/L]
EC10
(with 95 % confidence limits)
24
8.00
(Cl: Not applicable)
48
2.63
(Cl: 2.36 – 2.91)
EC50
(with 95 % confidence limits)
24
> 8.00
(Cl: Not applicable)
48
4.46
(Cl: 4.26 – 4.70)
EC100
24
> 8.00
48
> 8.00
NOEC
48
2.00
LOEC
48
4.00
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