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EC number: 268-544-3 | CAS number: 68123-13-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Endpoint summary
Administrative data
Link to relevant study record(s)
Description of key information
Basic Blue 99 have very low oral absorption and excretion, and expected to have Low bio-accumulation potential based on study results.
Key value for chemical safety assessment
- Bioaccumulation potential:
- low bioaccumulation potential
- Absorption rate - oral (%):
- 2.8
- Absorption rate - dermal (%):
- 10.1
Additional information
In a report given by Cosmetic Ingredient Review expert (2007), in vivo absorption of Basic Blue 99 (14C labeled) evaluated in three Sprague-Dawley rats treated topically with 200 μl of an aqueous Basic Blue 99 (14C labeled) hair setting solution. Levels of14C were low in the urine (< 0.02%) and feces (<0.07%) of two rats. The third rat excreted more than 5.05 % of14C dose in urine, but only 0.12% in feces.
In a similar report, two female volunteers treated topically with14C-labeled Basic Blue 99 added in a setting lotion (40% aqueous solution containing 0.1% of other Arianor dyes) and applied with rollers to the hair of the volunteers. Levels of14C in the urine were low in both volunteers (0.037% and 0.027%). The penetration would be less than 0.1% of the applied dose.
In a similar report, Wistar 2 male and 2 female rats were treated topically with 100 μl of an aqueous 1% Basic Blue 99 (14C labeled) solution. Total amount of14C Basic Blue 99 that penetrated the skin was 0.14% in male and 0.06% female. The highest absorption was observed in the anionic shampoo, then in the cationic and nonionic bases, and with the lowest penetration observed with the aqueous solution. However, even the highest absorption was very low and it is considered as insignificant.
In similar 4threport, Wistar 4 female rats were treated topically with 100 μl of an aqueous 1% Basic Blue 99 (14C labeled) solution in a 50% aqueous anionic shampoo base. Small amounts of14C were detected in the urine (1.16%), feces (0.53%), and carcass (0.03%). Only 1.72% of the applied dye penetrated the skin under occlusive conditions. Penetration was reduced in animals that were rinsed after application;14C was detected in the urine and feces (0.02%), blood and carcass (<0.001%), and only 2.9% of the applied material remained on the skin surface at 48 h after treatment. However, even the highest absorption was very low and it is considered as insignificant.
In similar 5threport, Wistar4 female rats were treated topically with 100 mg of 0.5% Basic Blue 99 (14C labeled) in nonionic/cationic shampoo base. Small amounts of14C were detected in the urine, feces, and carcass (0.5%) with <0.001% in blood. Penetration was reduced in animals that were rinsed after application;14C was detected in the urine (0.01%), feces (0.02%), blood and carcass (<0.001%), and 1.68% of the applied material remained on the skin surface at 48 h after treatment. Most of the radioactivity was recovered in the rinsing (99%) and 1.3% was on the patch.
In similar 6threport, Wistar 3 male and 3 female rats were treated with a single oral dosage of14C-labeled Basic Blue 99 (1.0 ml of an aqueous 0.1% solution). It was observed that most of the14C was recovered in the feces within the first 24 h. The urine contained 0.68% and 0.5% of the dose from male and female rats, respectively. Levels of14C were barely detectable in expired air and were very low in the blood and carcass (<0.001%). Basic Blue 99 is poorly absorbed from the intestinal tract.
In similar above report, in vivo absorption and Excretion of Basic Blue 99 were evaluated in Sprague-Dawley 2 male rats treated with a single oral dosage of (1 ml) 14C-labeled Basic Blue 99 and another two rats were administered Basic Blue 99 orally. Most of the14C was recovered in the feces in both study groups within the first 24 h. The urine contained 10.1% or 3.3% of the dose after intraperitoneal administration and 2.8% or 3.1% after oral administration. Basic Blue 99 was poorly absorbed.
Therefore, on the basis of above all observed result it is concluded that the Basic Blue 99 (CAS no 68123-13-7) has very low percutaneous or oral absorption and excretion, and expected to have Low bio-accumulation potential based on study results.
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