Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 205-271-0 | CAS number: 136-99-2
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The toxic effect of the test item to Daphnia magna was assessed in a static dose-response test. The 48-hour NOEC was determined to be 0.94 mg test item/L. The 48-hour LOEC was determined to be 2.07 mg test item/L and the 48-hour EC50 value was to be 4.02 mg test item/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 4.02 mg/L
Additional information
Young daphnids (< 24 hours old) were exposed in a static test to the test item for 48 hours, added to test water at a range of concentrations. After 48 hours of exposure no immobilisation of the test animals was observed in the control and up to the test item concentration of 0.94 mg test item/L. At the concentration of 2.07 mg test item/L, one daphnid was immobile. 12 immobile daphnids were observed at a test item concentration of 4.55 mg test item/L. At the highest test item concentrations of 10 mg test item/L, 20 daphnids were immobile.
The quantification of the test item was performed using liquid chromatography with mass detection (HPLCMS/MS method). At the start of the test 104 % of the nominal test concentration was found (average of all test concentrations at or higher than the NOEC). After 48 hours test duration, 105 % of the nominal value was determined (average of all test concentrations at or higher than the NOEC). During the test the daphnids were exposed to a mean of 104 % of nominal. Therefore, all reported results refer to nominal
concentrations.
The 48-hour NOEC was determined to be 0.94 mg test item/L. The 48-hour LOEC was determined to be 2.07 mg test item/L and the 48-hour EC50 value was to be 4.02 mg test item/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
