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Diss Factsheets
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EC number: 414-310-2 | CAS number: 191358-81-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994-10-18 to 1994-10-28
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study performed under GLP according to OECD guideline 209 (adopted 1984). Test duration was 30 minutes, however, for test items which do not rapidly degrade the test duration should be 3 h according to OECD guideline 209 adopted 2010.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted 4 April 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- adopted 30 May 1988
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test article was directly weighed into the test flasks and slurried with small portions of bidistilled water under stirring until a volume of about 200 mL was reached. These mixtures were treated in an ultrasonic bath for 15 minutes before adding water and 16 ml of synthetic sewage feed to reach a volume of 300 ml. Finally, 200 mL of microbial inoculum (activated sludge) was added.
- Controls: three controls
- Chemical name of vehicle: no vehicle used
- Evidence of undissolved material: The test article remained undissolved down to the lowest concentration of 10 mg/L.
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Source of sludge: aerobic activated sludge from a domestic waste water treatment plant (ARA Ergolz II, Füllinsdorf / Switzerland)
- Preparation of inoculum for exposure: The sludge was centrifuged and the supernatant liquid phase decanted. The solid material was resuspended in tap water and again centrifuged. The latter procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge (g) to its dry weight (g) determined. Based on this ratio, a volume of washed sludge suspension, corresponding to 4 g dry material, was made up to one litre with Soerensen buffer (pH 7). To this mixture, 50 ml OECD recommended synthetic sewage feed was added and the sludge was aerated at room temperature until use. Before use, the pH was measured to be 7.9.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Post exposure observation period:
- No data.
Test conditions
- Test temperature:
- 22°C
- pH:
- Starting pH 7.9
- Nominal and measured concentrations:
- Nominal test concentrations: 0, 10, 20, 32, 50 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 5 flasks with the test substance, 5 flasks with the reference compound and three control flasks
- Material, size, headspace, fill volume: > 500 mL glass flasks filled up to 500 mL
- Aeration: 30 min at 22 °C; flasks aerated with an air-flow about 0.2 L/min
- No. of vessels per concentration (replicates): one
- No. of vessels per control (replicates): three
- No. of vessels per vehicle control (replicates): none
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Oxygen consumption was measured over a period of about 10 minutes. During measurement, the sample was not aerated but continuously stirred on a magnetic stirrer. The apparatus used for oxygen measurements was the WTW 0X1 91 (Wissenschaftlich-Technische Werkstaetten, Weilheim/Germany). The pH was determined with the pH 90 (WTW Weilheim/Germany). - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The test substance remained undissolved down to the lowest concentration of 10 mg/L.
- The variance between the control samples measured before and after the reference compound was < 15 % (i.e. 1 %). - Results with reference substance (positive control):
- - Results with reference substance valid? Yes:
- Relevant effect levels: At the two lowest concentrations of 1.0 and 3.2 mg/l, the respiration was inhibited by 4.6 and 7.8 %, respectively. At 10 and 32 mg/l, the respiration was inhibited by 25.5 and 74.2 %, respectively. At 50 mg/l, the respiration rate was inhibited by 80.0 %. The EC50 determined by linear regression was calculated to be 15.1 mg/l.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriate low concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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