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EC number: 203-185-8 | CAS number: 104-21-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000-04-12 to 2000-04-14
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The nominal stock solution of 100 mg/L was prepared with dilution water one day prior to application and filtered (45p) before use.
- Stock solution: 100 mg/L, prepared with dilution water one day prior to application and filtered (45 µm) before use.
- Controls: Dilution water without test item tested under the same conditions as the test groups. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: Straus (Clone 5)
- Age at study initiation: Less than 24 h old
- Method of breeding: In 2-3 L glass vessels with approximatively 1.8 L culture medium, by 21 °C (temperatures of 20-25 °C were tolerated), in an incubator, 16 h illumination, illumination strength 1.5-5.0 µmol* mE-2 * sE-1 ( 100 - 200 lx).
- Food type: mix of Scenedesmus subspicatus and Chlorella vulgaris, with an algae cell density of > 10E+06 cells/mL.
- Frequency: 5 x weekly ad libitum
ACCLIMATION
- Acclimation period: 2 h in dilution water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 238 mg/L CaCO3 (dilution water)
- Test temperature:
- 21 ± 1 °C constant
- pH:
- Control: 7.59; 48 h: 7.53-7.63
10 mg/L: 7.38; 48 h: 7.42-7.55
18 mg/L: 7.49; 48 h: 7.39-7.52
32 mg/L: 7.51; 48 h: 7.45-7.53
58 mg/L: 7.39; 48 h: 7.42-7.51
100 mg/L: 7.4; 48 h: 7.35-7.49 - Dissolved oxygen:
- Control: 8.61 mg/L ; 48 h: 7.48-7.61
10 mg/L: 8.53; 48 h: 7.31-7.51
18 mg/L: 8.38; 48 h: 6.92-7.49
32 mg/L: 8.45; 48 h: 7.21-7.63
58 mg/L: 8.27; 48 h: 6.85-7.61
100 mg/L: 8.51; 48 h: 7.36-7.46 - Salinity:
- not applicable
- Conductivity:
- 666 µS/cm (dilution water)
- Nominal and measured concentrations:
- 10 - 18 - 32 - 58 - 100 mg test item/L (nominal)
/-/-22.4-38.5-69.9 mg C/L (measured DOC at 0 h) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (5 cm ID x 8 cm H), 50 mL
- Type: open
- Fill volume: 20 mL
- Application: Once within 48 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to EEC 92/69 L383A C.2.
- Total organic carbon: 100 mg/L: 66.6 mg/L, 58 mg/L: 38.6 mg/L, 32 mg/L: 21.3 mg/L
OTHER TEST CONDITIONS
- Photoperiod: 16/8 h light/dark cycle
- Light intensity: Diffuse light, illumination range 1.5-5.0 µmol* mE-2 * sE-1
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study
- Test concentrations: 1 -100 mg/L (nominal concentration), set up in a geometric series with a factor of 10 (NON-GLP-state).
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate p.a. (0.58 - 1.0 - 1.8 - 3.2 - 5.8 mg/L)
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 31 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 28 - 34 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: See following table
- Observations on body length and weight: No body weight and length was determined.
- Other biological observations: No.
- Mortality of control: No.
- Adverse effects control: No immobilisation was observed for control animals.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No. All tested concentration Ievels were clearly dissolved throughout exposure.
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- - Results with reference substance valid: yes
- EC50: 2.4 (Cl 2.2-2.5) - Validity criteria fulfilled:
- yes
- Conclusions:
- An EC50 of 31 mg/L (48 h) was determined with a short-term toxicity test in daphnia.
- Executive summary:
In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the test item were determined according to OECD 202. The study was conducted under static conditions over a period of 48 h with the following concentrations: 10 - 18 - 32 - 58 - 100 mg/L (nominal). Twenty Daphnids were exposed to each concentration level and the control. The EC10 and EC50 values were determined by probit analysis and the EC100 value was deduced directly from the dose-response-relationship. The EC10, EC50 and EC100 values were derived for 24 and 48 h. The EC50 of 52 mg/L (24 h) and 31 mg/L (48 h) was determined for daphnia based on nominal concentration.
Reference
Percentage of daphnids incapable of swimming after 24 and 48 h of exposure (n 20)
Test substance |
Immobilisation [%] |
|||||||||
nominal concentration [mg/L] |
24 h |
48 h |
||||||||
|
Replicates |
|
Replicates |
|
||||||
|
1 |
2 |
3 |
4 |
MV |
1 |
2 |
3 |
4 |
MV |
100 |
80 |
80 |
60 |
80 |
75 |
100 |
100 |
100 |
100 |
100 |
58 |
40 |
60 |
80 |
40 |
55 |
100 |
80 |
100 |
100 |
95 |
32 |
60 |
40 |
40 |
40 |
45 |
60 |
60 |
60 |
60 |
60 |
18 |
0 |
20 |
0 |
0 |
5 |
0 |
20 |
0 |
0 |
2 |
10 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
MV = Mean value
Description of key information
An EC50 of 31 mg/L (48 h) was determined with a short-term toxicity test in daphnia.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 31 mg/L
Additional information
In an acute immobilisation test with Daphnia magna (STRAUS), the effects of the test item were determined according to OECD 202. The study was conducted under static conditions over a period of 48 h with the following concentrations: 10 - 18 - 32 - 58 - 100 mg/L (nominal). Twenty Daphnids were exposed to each concentration level and the control. The EC10 and EC50 values were determined by probit analysis and the EC100 value was deduced directly from the dose-response-relationship. The EC10, EC50 and EC100 values were derived for 24 and 48 h. The EC50 of 52 mg/L (24 h) and 31 mg/L (48 h) was determined for daphnia based on nominal concentration.
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