1H-Imidazolium, 3-ethyl-1-methyl-, chloride (1:1)

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP and guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

65039-09-9

IUPAC Name:

65039-09-9

Constituent 2

Reference substance name:

1-Ethyl-3-methyl-1-imidazolium chloride (EMIM CI)

IUPAC Name:

1-Ethyl-3-methyl-1-imidazolium chloride (EMIM CI)

Details on test material:

- Name of test material (as cited in study report): 1-Ethyl-3-methyl-1-imidazolium chloride (EMIM CI)
- Analytical purity: 97.4 g/100g
- Lot/batch No.: EMIMCI 31F221105

Method

Target gene:

S. typhimurium and E. coli

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2

Metabolic activation:

with and without

Metabolic activation system:

Aroclor-induced rat liver S9 mix

Test concentrations with justification for top dose:

20 ug - 5000 ug/plate (dose levels for Standard Plate Test and Preincubation Test)

Vehicle / solvent:

Due to the good solubility of the test substance in water, water was used as vehicle.

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

yes

Positive controls:

yes

Remarks:

with metabolic activation: 2-aminoanthracene; without metabolic activation: N-methyl-N'-nitro-N-nitrosoguanidine, 4-nitro-o-phenylendiamine, 9-aminoacridine and 4-nitroquinoline-N-oxide

Details on test system and experimental conditions:

Standard Plate Test and Preincubation Test

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

other: A weak bacteriotoxic effect was occasionally observed using strain TA 1537 depending on the test conditions at 5000 ug/plate.

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

A weak bacteriotoxic effect was occasionally observed using strain TA 1537 depending on the test conditions at 5 000 μg/plate.
No precipitation of the test substance was found.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

According to the results of the present study, the test substance is not mutagenic in the Salmonella typhimurium / Escherichia coli reverse mutation assay under the experimental conditions chosen here.