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EC number: 248-236-5 | CAS number: 27121-30-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The test was conducted according to relevant guidelines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: Directive 88/302/EEC
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
Test material
- Reference substance name:
- [29H,31H-phthalocyaninetrisulphonyl trichloridato(2-)-N29,N30,N31,N32]copper
- EC Number:
- 248-236-5
- EC Name:
- [29H,31H-phthalocyaninetrisulphonyl trichloridato(2-)-N29,N30,N31,N32]copper
- Cas Number:
- 27121-30-8
- Molecular formula:
- C32H13Cl3CuN8O6S3
- IUPAC Name:
- Copper, [29H,31H-phthalocyanine-C,C,C-trisulfonyl trichloridato(2-)-kN29,kN30,kN31,kN32]-
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Details on test solutions:
- A stock solution (nominal concentration 2000 mg/L) was prepared. 200 mg/L test were placed in a beaker in mixed with 800 mL water. After stirring for 1.5 hours at 19°C, the test solution was adjusted with diluted sodium hydroxide from pH 2.1 to 7.0 und filled up to 1 L.
Nominal concentrations of 250 mg/L, 500 mg/L and 1000 mg/L were tested.
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Source: municipal sewage treatment works of Frankfurt/M-Sindlingen
Pretreatment: according to the guideline
Acclimatization: Not pre-adapted in the laboratory to the sumission substance or the reference substance
Study design
- Limit test:
- no
Test conditions
- Test temperature:
- 20°C ± 2°C
- pH:
- 7.2 ± 0.2
- Nominal and measured concentrations:
- nominal concentrations: 250 mg/L, 500 mg/L, 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers, diameter 60 mm, height 170 mm, volume appr. 480 mL - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol: 5, 20, 30 mg/L
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The submission substance is not harmful to activated sludge micororganisms. The EC50 is in the range of >1000 mg/L (nominal concentration).
- Results with reference substance (positive control):
- The EC50 (3 hours) for the reference substance was in the required range of the guideline.
- Reported statistics and error estimates:
- no data
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In an activated sludge respiration inhibition test, the submission substance proved to be innooxious to activated sludge microorgansims. The EC50 (3 hours) for respiration inhibition had been determined to be >1000 mg/L.
- Executive summary:
In an activated sludge respiration inhibition test, the submission substance proved to be nontoxic to activated sludge microorgansims. The test was conducted according to relevant guidelines. However, documentation is limited (reliability 2). The EC50 (3 hours) for respiration inhibition had been determined to be >1000 mg/L.
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