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EC number: 274-230-7 | CAS number: 69943-66-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted according to internationally accepted testing guideline.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- GLP compliance:
- no
Test material
- Reference substance name:
- Trisodium [3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-2-hydroxy-5-nitrobenzenesulphonato(3-)][2-hydroxy-3-[(2-hydroxy-1-naphthyl)azo]-5-nitrobenzenesulphonato(3-)]chromate(3-)
- EC Number:
- 274-230-7
- EC Name:
- Trisodium [3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-2-hydroxy-5-nitrobenzenesulphonato(3-)][2-hydroxy-3-[(2-hydroxy-1-naphthyl)azo]-5-nitrobenzenesulphonato(3-)]chromate(3-)
- Cas Number:
- 69943-66-4
- Molecular formula:
- C32H18CrN8O14S2Na3
- IUPAC Name:
- Trisodium [3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H pyrazol-4-yl)azo]-2-hydroxy-5-nitrobenzenesulphonato(3-)][2hydroxy-3-[(2-hydroxy-1-naphthyl)azo]-5-nitrobenzenesulphonato(3-)]chromate(3-)
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Spolkem breeder of Hrace Králové.
- Weight at study initiation: above 2.5 kg.
- Housing: individually housed, in commercially metal cages (supplied by s.p. Velaz Prague), without litter and climatic control.
- Diet: standard granular commercial food for rabbits, KO-16 provided by s.p. Velaz Prague.
- Water: drinking water, ad libitum.
Cleaning and disinfection of premises menagerie were made at dates determined, according to the standard operating procedures and compliance regime measures.
ENVIRONMENTAL CONDITIONS
- Temperature: 20 ± 3 °C.
- Humidity: 50 ± 15 %.
- Photoperiod: 12 hrs dark / 12 hrs light, by fluorescent lamp.
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- other: intact and abraded skin
- Vehicle:
- water
- Amount / concentration applied:
- TEST MATERIAL
- Amoun applied: 0.5 g - Duration of treatment / exposure:
- 24 hours
- Observation period:
- 72-hours
- Number of animals:
- 3 animals
- Details on study design:
- TEST SITE
- Area of exposure: 24 hours after the test material application, rabbits were shaved (skin area of 6 x 6 cm) on the left and right side of the lateral surface of the abdominal wall localized in the region epigastrium, mesogastrium and hypogastrium. Left side was intact, while the right side was scarificated.
- Type of wrap if used: test material was applied to skin on left side and covered with a gaude. Then water was applied to a gauze using a syringe, in order to obtain an aqueous paste. Furthermore on gauze were enclosed aluminium foil and cellulose wadding. The coverage was fixed by technical tape.
SCORING SYSTEM: OECD guideline scoring system.
Erythema and Eschar Formation
No erythema: 0
Very slight erythema (barely perceptible): 1
Well defined erythema: 2
Moderate to severe erythema: 3
Severe erythema (beef redness) to slight eschar formation (injuries in depth): 4
Maximum possible: 4
Oedema Formation
No oedema: 0
Very slight oedema (barely perceptible): 1
Slight oedema (edges of area well defined by definite raising): 2
Moderate oedema (raised approximately 1 mm): 3
Severe oedema (raised more than 1 mm and extending beyond area of exposure): 4
Maximum possible: 4
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- < 2.3
- Max. score:
- 4
- Remarks on result:
- other: both intact and abraded skin
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- < 2.3
- Max. score:
- 4
- Remarks on result:
- other: both intact and abraded skin
- Irritant / corrosive response data:
- Primary dermal irritation index (PDII): 0.5
Degree of dermal irritation: 2. Weakly irritant.
INTACT SKIN
Rabbit 1: a well-defined erythema and oedema.
Rabbit 2: very slight erythema and oedema very weak.
Rabbit 3: very slight erythema and oedema well defined.
For 24 hours were found very slight erythema and oedema very weak. At 48 and 72 hours no functional macroscopical, nor patomorphological changes were recorded.
SCARIFIED SKIN
Rabbit 1: a well-defined erythema and oedema.
Rabbit 2: very slight erythema and oedema very weak.
Rabbit 3: very slight erythema and oedema well defined.
At 48 and 72 hours no functional macroscopical, nor patomorphological changes were recorded.
Any other information on results incl. tables
Reactions observed
N. animal cage | Reaction | Reaction after | Mean 24, 48 and 72 hrs | |||
4 hrs | 24 hrs | 48 hrs | 72 hrs | |||
Intact skin | ||||||
1 | Erythema | 2 | 0 | 0 | 0 | 0.00 |
Oedema | 2 | 1 | 0 | 0 | 0.33 | |
2 | Erythema | 1 | 0 | 0 | 0 | 0.00 |
Oedema | 1 | 1 | 0 | 0 | 0.33 | |
3 | Erythema | 1 | 0 | 0 | 0 | 0.00 |
Oedema | 2 | 0 | 0 | 0 | 0.00 | |
Abraded skin | ||||||
1 | Erythema | 2 | 0 | 0 | 0 | 0.00 |
Oedema | 2 | 0 | 0 | 0 | 0.00 | |
2 | Erythema | 1 | 1 | 0 | 0 | 0.33 |
Oedema | 1 | 1 | 0 | 0 | 0.33 | |
3 | Erythema | 2 | 1 | 0 | 0 | 0.33 |
Oedema | 3 | 2 | 0 | 0 | 0.67 |
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information according to the CLP Regulation (EC 1272/2008) Criteria used for interpretation of results: EU
- Conclusions:
- Not irritating.
- Executive summary:
The skin irritation potential of the substance was determined by the skin irritation test, according to the OECD guideline 404.
0.5 g of test material were applied to intact and scarified skin of rabbits and removed after 24 hours. Observations were made for 72 hours.
Primary dermal irritation index (PDII) was determined to be 0.5 and the degree of dermal irritation was stated at 2.
The scoring system criteria used to record the hardness of the skin reactions followed in the current test are the same as those reported into the OECD guideline, therefore it is possible to re-evaluate the results according to the CLP Regulation (EC 1272/2008), because the raw tables are included into the study report.
Mean values from gradings at 24, 48 and 72 hours after patch removal were lower than 2.3 in all animals for both erythema/eschar and oedema reactions (in both intact and abraded skin).
Conclusion
The test item can be not classified as skin irritating, according to the CLP Regulation (EC 1272/2008).
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