Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 205-399-7 | CAS number: 140-11-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- fish, juvenile growth test
- Type of information:
- experimental study
- Remarks:
- Public literature
- Adequacy of study:
- key study
- Study period:
- 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Principles of method if other than guideline:
- Benoit DA, Holcombe GW, Spehar RL (1991) Guidelines for conducting early life toxicity tests with Japanese medaka (Oryzias latipes).
U.S. Environmental Protection Agency, Environmental Research Laboratory-Duluth. Ecological Research Series EPA-600/3-91-063, 25 pp - GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Water samples for chemical analyses were taken four times twice weekly during embryo-larval and 28-day larval tests. These water samples were taken alternately from each complete set of replicate tanks (6 tanks). The analyses of each set of 6 water samples included a replicated sample analysis for determination of percentage duplicate agreement, a spiked control sample and a series of analytical standards.
- Vehicle:
- no
- Details on test solutions:
- Stock solutions were prepared by dissolving the chemical in Lake Superior water using a high-speed stirrer. Stock solutions were prepared were prepared weekly depending on chemical stability. Stock solutions were transferred to a glass stock bottle inside the vented diluter enclosure using Teflon tubing and air pressure. During the test, a predetermined volume (mL/min) of stock solution was continuously pumped from the stock bottle into the mixing cell of the diluter system.
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- The medaka used for all tests were obtained from the Environmental Research Laboratory-Duluth (ERL-D) culture unit.
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 28 d
- Post exposure observation period:
- Not applicable
- Hardness:
- The mean and range for total hardness was 45.8 (38.0 - 52.0) mg/L as CaCO3 for all tests. The mean and range of alkalinity was 45.9 (35.0 - 58.5) mg/L as CaCO3 for all tests.
- Test temperature:
- 25 ± 1 °C
- pH:
- The arithmetic mean, standard deviation and range of pH readings for all tests was 7.88 ± 0.18 (7.31 - 8.85) N = 333
- Dissolved oxygen:
- Measurements obtained from all studies - 6.8 ± 0.7 (5.0 - 8.5) N = 339.
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Measured water concentrations:
28-day larval test: < 0.25, 0.27 ± 0.07, 0.47 ± 0.16, 0.92 ± 0.33, 1.92 ± 0.45, 4.85 ± 0.82 - Details on test conditions:
- Continuous-flow mini-diluter exposure systems with vented enclosures were used for all tests. The diluters generated five exposure concentrations and a control, all in replicate, with a dilution factor of 0.5. Exposure tanks were glass aquaria 18.5 x 14.0 x 13.0 cm deep. Each exposure tank had a 8.6 cm standpipe which resulted in a tank volume of 2.0 L. Flow rates and 90 % replacement times were (Sprague 1969) were 25 mL/min and 2.8 hours respectively, during all tests. Fluorescent lamps provided a light intensity that ranged from 12 to 25 lumens at the water surface. A 16-hour photoperiod was used for all tests.
The 28-day larval test was initiated by randomly distributing groups of 60 larvae. (0 - 3 days old) to each of the 12 exposure tanks. The test was started by placing groups of fish in tanks containing dilution water only and then starting the toxicant dosing pump so that appropriate test concentrations were achieved gradually within approximately 3 hours. Fish were fed live brine shrimp twice per day, Monday through Friday. Observations for determining larval survival were made daily. Dead organisms were removed during the daily tank cleaning procedure. - Duration:
- 28 d
- Dose descriptor:
- other: MATC
- Effect conc.:
- 1.33 mg/L
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.92 mg/L
- Details on results:
- Survival and growth of medaka were significantly reduced by benzyl acetate concentrations of 1.92 mg/L and above (Table 1). Based on these effects, the estimated MATC for benzyl acetate lies between 0.92 and 1.92 mg/L. The chronic value LC50 for benzyl acetate for medaka was 1.33 mg/L. NOEC was 0.92 mg/l
- Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Growth data and normalized hatch and survival data were analyzed using one-way analysis of variance with an F-test of significance (p = 0.05) and to
subsequent analysis with Dunnett's one-sided comparison (p = 0.05) of treatment means to control means (Steel and Torrie 1960). Mean chronic values were determined for all nine chemicals by calculating the geometric mean of the estimated MATC concentrations. - Validity criteria fulfilled:
- yes
- Conclusions:
- The chronic study with benzyl acetate were carried out under flow-through test conditions, test species was medaka (Oryzias latipes). The chronic MATC was calculated to be 1.33 mg/L.. NOEC value was 0.92 mg/l
- Executive summary:
This summary summarises a published paper where the toxicity of a number of materials were assess for acute (96 hours) and chronic (28 days) effects. Only the results of the studies with benzyl acetate are reported in this summary.
The study was conducted according to the protocol described in Benoit DA, et al (1991) Guidelines for conducting early life toxicity tests with Japanese medaka (Oryzias latipes). U.S. Environmental Protection Agency, Environmental Research Laboratory-Duluth. Ecological Research Series EPA-600/3-91-063, 25 pp
The chronic study was carried out under flow-through test conditions, test species was medaka (Oryzias latipes). Measured concentrations were 0.27, 0.47, 0.9, 1.92 and 4.85 mg/L, corresponding 28 day weights were 30.6 mg in the control and 30.3, 31.2, 31.9, 7.65 and 1.88 mg in the test treatments.
The chronic MATC value was calculated to be 1.33 mg/L. The NOEC value was 0.92 mg/l
Reference
|
Control |
Tank No. 2 |
Tank No. 3 |
Tank No. 4 |
Tank No. 5 |
Tank No. 6 |
28-Day Larval Test |
||||||
Measured Water Concentrations |
< 0.25a |
0.27 ± 0.07b |
0.47 ± 0.16 |
0.92 ± 0.33 |
1.92 ± 0.45 |
4.85 ± 0.82 |
% Survivald |
98.3 |
95.8 |
99.2 |
98.3 |
19.2 |
6.6 |
28-Day Weights (mg) |
30.6 ± 10.1 |
30.3 ± 10.5 |
31.2 ± 10.7 |
31.9 ± 12.0 |
7.65 ± 4.71e |
1.88 ± 0.64e |
aDetection limit of the analytical measurement procedure
bMean± standard deviation
cBased on 20 organisms exposed per concentration
dBased on 120 organisms exposed per concentration
eSignificantly different than controls (p=0.05)
Description of key information
This summary summarises a published paper where the toxicity of a number of materials were assessed for acute (96 hours) and chronic (28 days) effects. Only the results of the studies with benzyl acetate are reported in this summary.
The study was conducted according to the protocol described in Benoit DA, et al (1991) Guidelines for conducting early life toxicity tests with Japanese medaka (Oryzias latipes). U.S. Environmental Protection Agency, Environmental Research Laboratory-Duluth. Ecological Research Series EPA-600/3-91-063, 25 pp
The chronic study were carried out under flow-through test conditions, test species was medaka (Oryzias latipes). Measured concentrations were 0.27, 0.47, 0.9, 1.92 and 4.85 mg/L, corresponding 28 day weights were 30.6 mg in the control and 30.3, 31.2, 31.9, 7.65 and 1.88 mg in the test treatments.
The chronic MATC value was calculated to be 1.33 mg/L. The NOEC value was 0.92 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- NOEC
- Effect concentration:
- 0.92 mg/L
Additional information
The chronic study were carried out under flow-through test conditions, test species was medaka (Oryzias latipes) were exposed to benzyl acetate. Measured concentrations were 0.27, 0.47, 0.9, 1.92 and 4.85 mg/L, corresponding 28 day weights were 30.6 mg in the control and 30.3, 31.2, 31.9, 7.65 and 1.88 mg in the test treatments.
The chronic MATC value was calculated to be 1.33 mg/L. The NOEC value was 0.92 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.