Salicylic acid

Specific investigations: other studies

Administrative data

Endpoint:

specific investigations: other studies

Type of information:

experimental study

Adequacy of study:

other information

Study period:

data not available

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Non-standard study

Data source

Reference

Reference Type:

publication

Title:

Effects of chronic salicylate on GABAergic activity in rat inferior colliculus

Author:

Bauer CA Brozoski TJ, Holder TM, Caspary DM

Year:

2000

Bibliographic source:

Hearing Research, 147, 175-182.

Materials and methods

Test guideline

Qualifier:

no guideline followed

GLP compliance:

no

Type of method:

in vivo

Test material

Test material information

Constituent 1

Reference substance name:

no data available

IUPAC Name:

no data available

Details on test material:

"Salicylate"

Test animals

Species:

rat

Strain:

Long-Evans

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: no data
- Age at study initiation: between 7 and 12 weeks
- Weight at study initiation: no data
- Fasting period before study: no data
- Housing: individually in cage
- Diet : no data
- Water : no data
- Acclimation period: no data


ENVIRONMENTAL CONDITIONS
no data


IN-LIFE DATES: no data

Administration / exposure

Route of administration:

oral: drinking water

Vehicle:

not specified

Details on exposure:

PREPARATION OF DOSING SOLUTIONS: no data

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

4 months (animals behaviorally tested for evidence of tinnitus)
4 weeks (animals not behaviorally tested for evidence of tinnitus)

Frequency of treatment:

daily

Post exposure period:

no

Doses / concentrations

Dose / conc.:

8 other: mg/ml

Remarks:

Basis: nominal in water

No. of animals per sex per dose:

10 males per dose (behaviorally tested for evidence of tinnitus)
5 males per dose (no behaviorally tested for evidence of tinnitus)

Control animals:

yes, concurrent no treatment

Examinations

Examinations:

The following parameters were observed: daily fluid consumption, weekly weights, serum salicylate levels, auditory brainstem response thresholds,
GAD (Glutamic Acid Decarboxylase) levels in the inferior colliculus and GABA receptor binding characteristics.

Positive control:

no

Results and discussion

Details on results:

The serum salicylate level ranged from 9.89 to 38.2 mg/dL. No detectable levels of salicylate were present in control sera. The total mean daily dose of salicylic acid ingested by the subjects that were not behoviorally tested was 563.2-661 mg/kg/d. The weight of the subject was stable throughout the duration of the study. Auditory brainstem response testing revealed a 3.8 dB difference in the
mean threshold. Western blotting revealed a significant 63% (p < 0.008) elevation of GAD levels in the inferior colliculus (IC) ofsalicylate-treated
subjects. GAD expression and GABAa receptor binding characteristics may be altered with chronic exposure to sodium salicylate and these changes may represent aberrant plasticity clinically experienced as tinnitus.