Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-876-1 | CAS number: 100-66-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Although GLP were not mentioned, validity criteria and details on result were not available, the study was performed according the OECD test guideline and was generated by the Japanese Competent Authorities.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Preparation of activated sludge: A mix of old and fresh activated sludge is performed every three months. Fresh activated sludge is a mixture of ten samples from different cities and industrial sewage plants, soils and rivers in Japan. This mixture was then cultured at pH=7+/-1 under aeration
Concentration: 30 mg/L
No other information on the inoculum available. - Duration of test (contact time):
- 2 wk
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- other: BOD
- Details on study design:
- TEST CONDITIONS: no data
TEST SYSTEM
- Volume of test solution: 300 mL
- Number of culture flasks/concentration: 3
- magnetic stirrer
- Test temperature: 25°C +/- 1°C
- Culture medium: according to Japanese Industrial Standards JIS K 0102-1986-21 adjusted to pH = 7
No other data
SAMPLING: No data
CONTROL AND BLANK SYSTEM
- Inoculum blank: 1 test vessel with 300mL of basal culture medium (sludge)
- water + test substance at 100 mg/L
- reference substance: sludge + aniline at 100 mg/L
STATISTICAL METHODS: no data - Reference substance:
- aniline
- Parameter:
- other: BOD
- Value:
- 56
- Sampling time:
- 2 wk
- Results with reference substance:
- With aniline, percentages of biodegradation calculated by BOD value were beyond 40% and 60% after 7 and 14 days respectively.
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Although the biodegradation observed does not strictly enable to conclude on the ready biodegradability profile of the substance, because the biodegradation is below the pass level of 60% and no measurements are available between 14 and 28 days, it is however possible to conclude that the substance is readily biodegradable base on the following two aspects:
- the biodegradation observed at 14d is very closed to the pass level for ready biodegradability at 28d. Therefore, it is anticipated that this pass level would have been fulfilled
- the 10 -d window does not apply to the MITI method.
Reference
In a 2-week biodegradation screening test (MITI test) of Anisole (100 mg/L) using an activated sludge inoculum, 56% of BOD was removed. At the end of the 2-week period there was an upward trend in BOD consumption.
Description of key information
Anisole has been demonstrated to be readily biodegradable in screening test.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
A reliable key study is available for ready biodegradability. During 14 days, the biodegradation of anisole at an initial concentration of 100 mg/L was studied according to OECD guideline 301C.
Inoculum used in the test was an activated sludge at a concentration of 30 mg/L obtained by mixing 10 different samples from different city and industrial sewage plants, soils and rivers in Japan.
The percentage of biodegradation was estimated by the Biological Oxygen Demand (BOD).
The corresponding percentage of biodegradation obtained after 14 days was 56%.
Although this value does not strictly enable to conclude on the ready biodegradability profile of the substance, because the biodegradation is below the pass level of 60% and no measurements are available between 14 and 28 days, it is however possible to conclude that the substance is readily biodegradable base on the following two aspects:
- the biodegradation observed at 14d is very closed to the pass level for ready biodegradability at 28d. Therefore, it is anticipated that this pass level would have been fulfilled
- the 10 -d window does not apply to the MITI method.
In conclusion, under the test conditions anisole is readily biodegradable.
Although GLP were not mentioned, validity criteria and details on results were not available, this study was performed according to OECD test guideline and was generated by the Japanese Competent Authorities. This study is therefore considered as reliable with acceptable restriction.
Three additionnal data of unknown validity support this conclusion.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.