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Diss Factsheets
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EC number: 215-676-4 | CAS number: 1341-49-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian germ cell study: gene mutation
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Published study, poorly reported; non-standard methodology
Data source
Reference
- Reference Type:
- publication
- Title:
- The influence of atmospheric hydrogen fluoride on the frequency of sex-linked recessive lethals and sterility in Drosophila melanogaster
- Author:
- Gerdes RA
- Year:
- 1 971
- Bibliographic source:
- Fluoride 4(1): 25-29
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Exposure to 3 concentrations of hydrogen fluoride vapour to determine sex-linked recessive lethal mutations and sterility levels in Drosophila melanogaster.
- GLP compliance:
- no
- Remarks:
- : older publsihed study, pre-dates GLP
- Type of assay:
- Drosophila SLRL assay
Test material
- Reference substance name:
- Hydrogen fluoride
- EC Number:
- 231-634-8
- EC Name:
- Hydrogen fluoride
- Cas Number:
- 7664-39-3
- IUPAC Name:
- fluoride
- Details on test material:
- No further information.
Constituent 1
Test animals
- Species:
- Drosophila melanogaster
- Strain:
- other: Oregon-r
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Oregon-r stocks of Drosophila melanogaster adults (8-12 hours old). Populations were maintained at stable densities by removing in sequence the food vials. Virgin "Basc" females were used to find sex-linked lethals.
Administration / exposure
- Route of administration:
- inhalation: vapour
- Vehicle:
- No vehicle was used.
- Details on exposure:
- Drosophila were exposed to hydrogen fluoride vapour in a fumigation chamber.
- Duration of treatment / exposure:
- 24 hours for the sex-linked recessive lethal test. Sterility testing was conducted after 0, 3 and 6 weeks exposure.
- Frequency of treatment:
- Continuous during exposure period.
- Post exposure period:
- Matings were carried out over three 3-day brood periods. Egg samples were collected at 0, 3 and 6 weeks from initiation of fumigation.
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0, 1.3, 2.9 and 4.3 ppm
Basis:
nominal conc.
vapour
- No. of animals per sex per dose:
- No information.
- Control animals:
- yes, concurrent no treatment
- Positive control(s):
- Not examined.
Examinations
- Tissues and cell types examined:
- Not applicable.
- Details of tissue and slide preparation:
- Not applicable.
- Evaluation criteria:
- Sex-linked recessive lethals: The F1 generation from the treated males and Basc females were allowed to intermate and the females then sorted singly into vials. The absence of wild type males in the F2 generation was scored as a lethal.
Sterility: Each treated male and female was mated individually to a tester and these crosses were scored after 5 days for the presence of larvae. If no larvae were present the treated parent was transferred to a new vial and remated to a tester. After an additional 5 day period the flies in the vials without larvae were scored as sterile. - Statistics:
- Lethal frequencies: Pearson and Hartley test for significance of observed differences between two Poisson variables.
Sterility: expressed as the percentage failure of adult Drosophila to produce viable larvae, ANOVA was used to determine significance of differences.
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- positive
- Remarks:
- 2.9 ppm and above
- Toxicity:
- not examined
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- not examined
- Additional information on results:
- No additional information.
Any other information on results incl. tables
The lethal frequency results of the three 3 -day broods were pooled. 1.3ppm hydrogen fluoride caused a non-significant increase in mutation frequency (0.015%) compared to controls. Significant increases in mutation frequency were observed at 2.9 and 4.3ppm concentrations. The percentage sterility was increased for both males and females, sterility increased with time and with concentration.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): ambiguous
Sub-lethal exposures to hydrogen fluoride vapour resulted in genetic aberrancies in Drosophila melanogaster, however the study was poorly reported therefore the significance of the results cannot be evaluated. - Executive summary:
Drosophila melanogaster were exposed to hydrogen fluoride vapour to investigate levels of mutagenicity caused by the substance. Significant increases in mutation frequency were observed at 2.9 and 4.3 ppm concentrations (24 hour exposure). The percentage sterility was increased for both males and females, sterility increased with time exposed (0, 3 and 6 weeks) and with concentration. The author concluded that sex-linked recessive lethals indicated an effect upon a specific chromosome, whilst the increase in sterility levels resulted from a broad effect upon the genome as a whole. However it must be noted that the study was poorly reported and therefore the significance of these results cannot be evaluated.
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