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EC number: 237-898-0 | CAS number: 14059-33-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
Two Ames tests (similar to OECD guideline 471), an in vitro mammalian chromosome aberration test (similar to OECD guideline 473), an in vitro mammalian HPRT test (similar to OECD guideline 476) and an in vivo micronucleus test with intraperitoneal exposure (according to OECD guideline 474) are available. All tests were negative.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Genetic toxicity in vivo
Description of key information
Two Ames tests (similar to OECD guideline 471), an in vitro mammalian chromosome aberration test (similar to OECD guideline 473), an in vitro mammalian HPRT test (similar to OECD guideline 476) and an in vivo micronucleus test with intraperitoneal exposure (according to OECD guideline 474) are available. All tests were negative.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
Ames
The test substance was tested for mutagenicity in the Ames test (standard plate test).
Strains: TA 1535, TA 100, TA 1537, TA 1538, TA 98
Dose range: 20 µg - 5000 µg/plate
Test conditions: Standard plate test with and without metabolic activation (S9 mix).
Solubility: Incomplete solubility of the test substance from 500 µg onward.
Toxicity: No bacteriotoxic effect (reduced his- background growth) was observed.
Mutagenicity: An increase in the number of his+ revertants could not be observed either without S9 mix or after addition of a metabolizing system.
Assessment: According to the results of the present study, the substance is thus not mutagenic in the Ames test under the experimental conditions chosen here. (BASF, 1983)
This test result is supported by the Ciba Ames test which comprised in addition to the S. typhimurium strains TA 1535, TA 1537, TA 98 and TA 100 also E. coli WP2 uvrA.
Chromosome Aberration Assay
An in vitro mammalian chromosome aberration test was conducted in Chinese hamster lung fibroblasts (V79 cells) with and without metabolic activation (S9 mix from treated SD rats). 0.5 % carboxymethyl cellulose (CMC) were used as solvent. Positive controls were MMC and CPA. Application of the test substance was in medium for an exposure duration of 24 and 48 hours without metabolic activation and 6 hours with metabolic activation. 100 - 200 cells were evaluated. Mitotic index and relative total growth were determined to evaluate cytotoxicity. No genotoxicity was observed with and without metabolic activation at concentrations up to 5000 µg/mL. Therefore, the test substance is considered to be non-mutagenic in the chromosome aberration assay under the test conditions chosen.
HPRT
The study was performed to investigate the potential of the test substance to induce gene mutations at the HPRT locus in V79 cells of the Chinese hamster. The assay was performed in two independent experiments, using two parallel cultures each. The main experiments were performed with and without liver microsomal activation and a treatment period of 4 hours. The highest concentration (3240 µg/mL) of the pre-experiment and both main experiments was equal to a molar concentration of approximately 10 mM. The test item was suspended in deionised water. Precipitation of the test item was observed in experiment 1 at 1620 µg/mL and above with and without metabolic activation. In experiment 2 precipitation was noted at 500.0 µg/mL and above with and without metabolic activation. No relevant cytotoxic effect indicated by a relative cloning efficiency or cell density below 50 % in both parallel cultures was observed up to the highest concentration of both experiments with and without metabolic activation. No substantial and reproducible dose dependent increase of the mutation frequency was observed up to the maximum concentration with and without metabolic activation. Appropriate reference mutagens (EMS and DMBA), used as positive controls, induced a distinct increase in mutant colonies and thus, showed the sensitivity of the test system and the activity of the metabolic activation system. In conclusion it can be stated that under the experimental conditions reported the test item did not induce gene mutations at the HPRT locus in V79 cells. Therefore, the test substance is considered to be non-mutagenic in this HPRT assay.
Micronucleus Test in vivo
The in vivo micronucleus test was conducted with intraperitoneal
administration because the acute tests with the test material bismut
vanadate had pointed to a lower oral bioavailability of the test
compound compared to soluble vanadates. Soluble vanadates proved
positive in the micronucleus assay following oral administration,
however, the soluble vanadium pentoxide was negative following
inhalation exposure. To maximize the exposure to the poorly soluble
bismut vanadate, intraperitoneal administration was chosen which could
have revealed a micronucleus induction by even small amounts of
solubilized test material which would most likely have been missed by
oral and would surely have been missed by inhalation exposure. The test
substance did not lead to an increase in the number of polychromatic
erythrocytes containing either small or large micronuclei up to doses of
2000 mg/kg bw. The PCE/NCE ratio was uneffected, i.e. no inhibition of
erythropoiesis was induced by the test substance.
Justification for classification or non-classification
Classification, Labelling, and Packaging Regulation (EC) No. 1272/2008
The available experimental test data are reliable and suitable for classification purposes under Regulation 1272/2008. No adverse findings or genotoxicity was observed in in vitro or in vivo studies. As a result, the substance is not considered to be classified for mutagenicity under Regulation (EC) No. 1272/2008.
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