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EC number: 234-126-4 | CAS number: 10544-72-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Based upon a review of the available data the following effect level has been chosen for acute toxicity: inhalation:
LC50 (rat) at 60 mins for nitrogen dioxide: 115 ppm.
The modification of exposure duration to 4-hour exposure using Haber's law results in the 4-h LC50 for nitrogen dioxide of 28.8 ppm, or 55 mg/m3. Considering one mole dinitrogen tetraoxide results in the formation of two moles of nitrogen dioxide, this value was recalculated to 14.4 ppm by the registrant, which is equal to 55 mg/m3 , for dinitrogen tetraoxide.
Key value for chemical safety assessment
Acute toxicity: via oral route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Acute toxicity: via inhalation route
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LC50
- Value:
- 55 mg/m³ air
- Quality of whole database:
- The study has a Klimisch score 2.
Acute toxicity: via dermal route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Acute Toxicity: Oral and dermal
The substance is a gas and is classified as corrosive to skin. Therefore in accordance with column 2 of Annex VII (required in section 8.5) of REACH Regulation 1907/2006 the study does not need to be conducted.
Acute Toxicity: Inhalation
The substance is classified as corrosive to skin. Therefore in accordance with column 2 of Annex VIII (required in section 8.5) of REACH Regulation 1907/2006 the study does not need to be conducted. However, there is some literature data available for the test material (Peng et al, 2004) and also nitrogen dioxide (Carson et al, 1962), which can be read across to the test material based upon the following justification:
It is understood that there is an equilibrium existing between NO2 and N2O4. Hence, it would seem inevitable that any toxicity studies conducted with these oxides will share common toxicities. It also seems plausible that the prevalent oxide of nitrogen would be nitrogen dioxide.
The results obtained from the available literature data is as follows:
Dinitrogen Tetroxide:
Peng et al (2004)
The first 2 - 6 h after intoxication is the most severe stage of the injury. During the long-term observation intoxication with the test material can induce pulmonary fibrosis and adenocarcinoma.
Nitrogen dioxide:
Carson et al (1962)
Rat: Based on the lung to body weight ratio and the pathological changes found in exposed animals, the LC50 concentrations of the test item were 416, 201 and 115 ppm for 5, 15 and 60 min, respectively.
Rabbit: Rabbits were exposed for 15 minutes to various concentrations of the test item. The LC50 was estimated to be 315 ppm.
Dog: Dogs were exposed for single 5- to 60-minute periods to various concentrations of the test item. Dogs had only mild toxic signs to exposure to the test item.
Based upon a review of this data the following effect level has been chosen as the literature paper provides LC50 values and the preferred species (rat) is used:
LC50 (rat) at 60 mins: 115 ppm
The modification of the exposure duration from 60 minutes to 4 hours using Haber's law, as recommended by the ECHA guidance on information requirements and chemical safety assessment (cn x t = const), where n = 1 for the extrapolation from shorter to longer exposure durations, results in 4 -h LC50 of 28.8 ppm, or 55 mg/m3. Considering one mole dinitrogen tetraoxide results in the formation of two moles of nitrogen dioxide, this value was recalculated to 14.4 ppm for dinitrogen tetraoxide by the registrant, which is equal to 55 mg/m3.
Justification for selection of acute toxicity – oral endpoint
The substance is a gas and is classified as corrosive to skin.
Therefore in accordance with column 2 of Annex VII (required in section
8.5) of REACH Regulation 1907/2006 the study does not need to be
conducted.
Justification for selection of acute toxicity – inhalation endpoint
The substance is classified as corrosive to skin. Therefore in
accordance with column 2 of Annex VIII (required in section 8.5) of
REACH Regulation 1907/2006 the study does not need to be conducted.
However, there is some literature data available for the test material
(Peng et al, 2004) and also nitrogen dioxide (Carson et al, 1962), which
can be read across to the test material based upon the following
justification:
It is understood that there is an equilibrium existing between NO2 and
N2O4.Hence, it would seem inevitable that any toxicity studies conducted
with these oxides will share common toxicities. It also seems plausible
that the prevalent oxide of nitrogen would be Nitrogen Dioxide.
The results obtained from this literature data is as follows:
Dinitrogen Tetroxide:
Peng et al (2004)
The first 2 - 6 h after intoxication is the most severe stage of the
injury. During the long-term observation, we find that intoxication with
the test material can induce pulmonary fibrosis and adenocarcinoma.
Nitrogen dioxide:
Carson et al (1962)
Rat: Based on the lung to body weight ratio and the pathological changes
found in exposed animals, the threshold concentrations of the test item
found were 104, 65 and 28 ppm for 5, 15 and 60 min, respectively.
Rabbit: Rabbits were exposed for 15 minutes to various concentrations of
the test item. The LC50 was estimated to be 315 ppm.
Dog: Dogs were exposed for single 5- to 60-minute periods to various
concentrations of the test item. Dogs had only mild toxic signs to
exposure to the test item.
Based upon a review of this data the following effect level has been
chosen as the literature paper provides LD50 values and the preferred
species (rat) is used:
LC50 (rat) at 60 mins: 115 ppm
The modification of the exposure duration using Haber's law, as
recommended by the ECHA guidance on information requirements and
chemical safety assessment, to 4-hour exposure results in 4-h LC50 of
28.8 ppm, or 55 mg/m3.
Justification for selection of acute toxicity – dermal endpoint
The substance is a gas and is classified as corrosive to skin.
Therefore in accordance with column 2 of Annex VII (required in section
8.5) of REACH Regulation 1907/2006 the study does not need to be
conducted.
Justification for classification or non-classification
The substance is currently classified as Acute Tox. 2* according to Annex VI of Regulation (EC) No 1727/2008, however the asterix against it indicates that it is a "de minimis" classification made by conversion of the entries of Annex I of the DSD with other classification criteria in mg/L.
In the study of Carsson et al. (1962), 1 -hour LC50 of 115 ppm was obtained. The modification of the exposure duration to 4 hours using Haber's law, as recommended by the ECHA guidance on information requirements and chemical safety assessment, results in the 4 -h LC50 of 28.8 ppm, or 55 mg/m3. Considering one mole dinitrogen tetraoxide results in the formation of two moles of nitrogen dioxide, this value was recalculated to 14.4 ppm by the registrant, which is equal to 55 mg/m3 , or 0.055 mg/L for dinitrogen tetraoxide. Therefore the classification of dinitrogen tetraoxide as Acute Tox. 1, H330 in accordance with Regulation 1272/2008/EC is proposed by the registrant.
The harmonized classification of dinitrogen tetraoxide according to Regulation 1272/2008/EC gives a specific concentration limit of 0.5% for classification with STOT SE 3, H335. However, respiratory tract irritation is inherent to the corrosive properties of the substance and is considered therefore to be covered by classification as Skin Corr. 1B, H314 and EUH071 (Corrosive to respiratory tract). Therefore a separate classification as STOT SE 1, H335 is considered to be not necessary.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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