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EC number: 701-392-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-tetradecyloxirane, reaction products with boric acid
- EC Number:
- 701-392-2
- IUPAC Name:
- 2-tetradecyloxirane, reaction products with boric acid
- Details on test material:
- - Physical state: Light coloured liquid
- Analytical purity: Considered to be 100% pure
- Storage condition of test material: In the dark
- Other: MSDS and sample receipt indicate the sample recieved as a solid.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- Not applicable for this type of study.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test material was added directly to each test vessel using glass microscopic cover slips. Nominal test material concentrations of 1, 10, 100, 1000 and 10000 mg/L corresponding to 0.0005, 0.005, 0.05, 0.5 and 5 g of test material were used.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - On day or collection and arrival, sludge aerated with low-pressure, oil free air and fed with synthetic feed at a rate of 50 mL /L
- Triplicate 4 mL samples were dried at 100°C to a constant weight, then diluted to produce a dryweight per unit volume concentration of 4 g/L
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- Not applicable
Test conditions
- Hardness:
- Not applicable
- Test temperature:
- 20°C
- pH:
- Not applicable
- Dissolved oxygen:
- Not applicable
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal test material concentrations of 1, 10, 100, 1000 and 10000 mg/L corresponding to 0.0005, 0.005, 0.05, 0.5 and 5 g of test material were used.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1L flint glass bottles
- Total fill volume: 500 mL
- Type : closed
- Aeration: yes, low pressure oil free air by glass pipet
- No. of vessels per concentration (replicates): 1 concentration
- No. of vessels per control (replicates): 2 x reference substance concentration + 1 blank control
- Biomass loading rate: 200 mL sludge
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: softened and dechlorinated tap water
- Culture medium different from test medium: No
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: maintained in dark (24h)
EFFECT PARAMETERS MEASURED :
Oxygen consumption - Reference substance (positive control):
- yes
- Remarks:
- 3, 5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: The NOEC value is assigned on the basis of the low discriminatory power of the statistical analysis and is not an experimentally determined value
- Duration:
- 3 h
- Dose descriptor:
- IC10
- Effect conc.:
- 2.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CI = 0.1 - 5.4 mg/L
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels:
40 mg/L - respiration rate = 0.8 mg/L, (86.6% inhibition)
20 mg/L - respiration rate = 22.1 mg/L (62.9% inhibition)
10 mg/L - respiration rate = 45.8 mg/L (23.0% inhibition)
The EC50 for the reference substance was calculated to be 17.0 mg/L
- Other:
Any other information on results incl. tables
Results:
Test concentration (mg/L) |
Change in O2(mg/L) |
Change in time (mins) |
O2consumption (mg/L*hr) |
% inhibition |
1 |
2.2 |
2 5/6 |
46.6 |
21.7 |
10 |
2.8 |
3 2/6 |
50.5 |
15.1 |
100 |
2.1 |
2 5/6 |
44.5 |
25.2 |
1000 |
1.9 |
2 4/6 |
42.7 |
28.2 |
10000 |
2.0 |
3 |
40.0 |
32.8 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC 50 (3h) for sewage sludge respiration inhibition is > 10000 mg/L
- Executive summary:
Test Guidance
OECD Guideline 209
Method
The study assessed the potential for the test substance to inhibit aerobic microbial metabolism, as indicted by the consumption of oxygen. The test duration was a three-hour exposure period to the test material at five nominal dose concentrations (1, 10 , 100, 1000 and 10000 mg/L) followed by up to ten minutes for the measurement of oxygen consumption. A duplicate control group and a positive control using a reference toxicant, 3,5 -dichlorophenol were run simultaneously with the test group.
Results
The quality control criteria for the test were acceptable. The EC50 for the reference substance was 17 mg/L and the two control replicants produced oxygen consumption rates that were within 15% of each other. The EC50 was calculated to be greater than 10000 mg/L. The statistically derived NOEC is 1 mg/L but the calculation is not sufficently robust.
Conclusion
The test material is not harmful to sewage sludge micro-organisms
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