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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 915-035-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / micronucleus study
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable study report which meets basic scientific principles.
Data source
Reference
- Reference Type:
- publication
- Title:
- In vitro genotoxic effects of different combinations of cobalt and metallic carbide particles
- Author:
- De Boeck, M., N. Lombaert et al.
- Year:
- 2 003
- Bibliographic source:
- Mutagenesis 18(2): 177-186
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: OECD 487
- Deviations:
- not specified
- Principles of method if other than guideline:
- All details of the method and experimental setting not described in detail.
- GLP compliance:
- not specified
- Type of assay:
- in vitro mammalian cell micronucleus test
Test material
- Reference substance name:
- Trichromium dicarbide
- EC Number:
- 234-576-1
- EC Name:
- Trichromium dicarbide
- Cas Number:
- 12012-35-0
- Molecular formula:
- Cr3C2
- IUPAC Name:
- trichromium dicarbide
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Cr3C2 chromium carbide
surface area 0.983 m2/g, median diameter 1.2 µm
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- lymphocytes: human PBMC
- Metabolic activation:
- not specified
- Test concentrations with justification for top dose:
- 10, 50, 100 µg/ml
- Vehicle / solvent:
- sterile deionized water
Controls
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- ethylmethanesulphonate
- mitomycin C
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium
DURATION
- Preincubation period: 24 h
- Exposure duration: 48 h
Cytochalasin B was added 44 h after the start of the culture
NUMBER OF REPLICATIONS: dublicates for each concentration
NUMBER OF CELLS EVALUATED:
DETERMINATION OF CYTOTOXICITY
- Method: relative division index
OTHER EXAMINATIONS:
- Comet assay - Evaluation criteria:
- Duplicate samples of 1000 cytokinesisblocked (binucleated) lymphocytes were examined for the presence of micronuclei.
For the Comet assay, % of DNA in the tail and tail length were recorded as DNA damage parameters. - Statistics:
- Student's t-test for differences between treated and control samples (P<0.0083 for statistical significance). Dose-dependency was tested by a trend test (P<0.0025 for statistical significance).
Results and discussion
Test results
- Species / strain:
- lymphocytes:
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks:
- reporting unclear; no statistical analysis on relative division index?
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Trichromium dicarbide at concentrations of 10, 50 and 100 µg/ml did not cause any genotoxicity in human lymphocytes, as measured by the micronucleus test and the Comet assay. - Executive summary:
Exposure of isolated human lymphocytes cells with trichromium dicarbide at concentrations of 10, 50 and 100 µg/ml did not cause any genotoxicity, as measured by the micronucleus test and the Comet assay.
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