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EC number: 221-882-5 | CAS number: 3268-49-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- adopted July 21, 1997
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3-(methylthio)propionaldehyde
- EC Number:
- 221-882-5
- EC Name:
- 3-(methylthio)propionaldehyde
- Cas Number:
- 3268-49-3
- Molecular formula:
- C4H8OS
- IUPAC Name:
- 3-(methylthio)propionaldehyde
- Test material form:
- liquid
Constituent 1
Method
- Target gene:
- The Salmonella typhimurium histidine (his) and the Escherichia coli tryptophan (trp) reversion system measures his-→ his+ and trp-→ trp+ reversions, respectively.
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Cofactor supplemented post-mitochondrial fraction (S9 mix), prepared from the livers of rats treated with phenobarbital/ß-naphtoflavone.
- Test concentrations with justification for top dose:
- In a pre-experiment a concentration range of the test item was 3 – 5000 μg/plate. The pre-experiment is reported as experiment I. Since toxic effects were observed in experiment I, eight concentrations were tested in experiment II. 5000 μg/plate were chosen as maximal concentration. The concentration range included two logarithmic decades. The following concentrations were tested in experiment II: 3; 10; 33; 100; 333; 1000; 2500; and 5000 μg/plate.
Experiment 1 (plate incorporation test) and Experiment 2 (preincubation test): 3, 10, 33, 100, 333, 1000, 2500 and 5000 µg/plate, with and without metabolic activation - Vehicle / solvent:
- - Vehicle/solvent used: DMSO
- Justification for choice of solvent/vehicle: The solvent has been chosen according to its solubility properties and its relative nontoxicity to the bacteria.
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- methylmethanesulfonate
- other: 2-aminoanthracene dissolved in DMSO; for TA 1535, TA 1537, TA 98, TA 100, WP2 uvrA; +S9 // 4-nitro-o-phenylene-diamine, 4-NOPD dissolved in DMSO; for TA 1537, TA 98; -S9
- Details on test system and experimental conditions:
- NUMBER OF REPLICATIONS:
- Number of cultures per concentration: triplicate
METHOD OF TREATMENT/ EXPOSURE:
- Test substance added in agar (plate incorporation); preincubation
TREATMENT AND HARVEST SCHEDULE:
- Preincubation period: 60 min
- Exposure duration/duration of treatment: 48 hours at 37 C ± 1.5° C in the dark
METHODS FOR MEASUREMENT OF CYTOTOXICITY
- Method: background growth inhibition
METHODS FOR MEASUREMENTS OF GENOTOXICIY
- Method: Number of revertant colonies - Evaluation criteria:
- A test item is considered as a mutagen if a biologically relevant increase in the number of revertants of twofold or above (strains TA 98, TA 100, and WP2 uvrA) or threefold or above (strains TA 1535 and TA 1537) the spontaneous mutation rate of the corresponding solvent control is observed. A dose dependent increase is considered biologically relevant if the threshold is reached or exceeded at more than one concentration. An increase of revertant colonies equal or above the threshold at only one concentration is judged as biologically relevant if reproduced in an independent second experiment. A dose dependent increase in the number of revertant colonies below the threshold is regarded as an indication of a mutagenic potential if reproduced in an independent second experiment. However, whenever the colony counts remain within the historical range of negative and solvent controls such an increase is not considered biologically relevant.
- Statistics:
- Mean values and standard deviation were calculated.
Results and discussion
Test results
- Key result
- Species / strain:
- other: S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2 uvrA
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- in all strains at higher concentrations with and without metabolic activation
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation and time of the determination: No precipitation of the test item occurred in the overlay agar in the test tubes. Precipitation of the test item in the overlay agar on the incubated agar plates was observed at 5000 μg/plate.
STUDY RESULTS
- Concurrent vehicle negative and positive control data
Ames test:
- Signs of toxicity , Individual plate counts , Mean number of revertant colonies per plate and standard deviation : Refer to Table no. 1 and 2 under "Any other information on results incl. tables".
HISTORICAL CONTROL DATA
- Positive historical control data: yes
- Negative (solvent/vehicle) historical control data: yes
Any other information on results incl. tables
Table 1:
Summary of Experiment I | |||||||
Metabolic Activation | Test Group | Dose level per plate | Revertant Colony Count (Mean) | ||||
TA1535 | TA1537 | TA98 | TA100 | WP2uvRA | |||
without activation | DMSO | 20 | 10 | 24 | 138 | 39 | |
Untreated | 18 | 13 | 28 | 146 | 43 | ||
MMP | 3 µg | 16 | 8 | 25 | 127 | 40 | |
10 µg | 19 | 11 | 20 | 139 | 40 | ||
33 µg | 17 | 10 | 30 | 129 | 41 | ||
100 µg | 19 | 9 | 28 | 121 | 41 | ||
333 µg | 21 | 9 | 33 | 121 | 39 | ||
1000 µg | 23 | 12 | 21 | 138 | 54 | ||
2500 µg | 8 | 6 | 10 | 1 | 41 | ||
5000 µg | 1P | 1P | 1P | 0P | 2P | ||
NaN3 | 10 µg | 1612 | 2374 | ||||
4-NOPD | 10 µg | 378 | |||||
4-NOPD | 50 µg | 79 | |||||
MMS | 2.0 µL | 1085 | |||||
with activation | DMSO | 15 | 11 | 37 | 127 | 49 | |
Untreated | 19 | 16 | 34 | 128 | 56 | ||
MMP | 3 µg | 16 | 15 | 36 | 127 | 47 | |
10 µg | 19 | 10 | 39 | 126 | 47 | ||
33 µg | 15 | 11 | 37 | 134 | 48 | ||
100 µg | 19 | 10 | 41 | 126 | 55 | ||
333 µg | 19 | 13 | 38 | 107 | 52 | ||
1000 µg | 20 | 13 | 29 | 99 | 48 | ||
2500 µg | 20 | 8 | 23 | 96 | 52 | ||
5000 µg | 2P | 3P | 1P | 1P | 34P | ||
2-AA | 2.5 µg | 362 | 369 | 3793 | 4827 | ||
2-AA | 10.0 µg | 222 |
P: Precipitate, NaN3: sodium azide, 2 -AA: 2- aminoanthracene, 4 -NOPD: 4 -nitro-o-phenylene-diamine, MMS: methyl methane sulfonate
Table 2:
Summary of Experiment II | |||||||
Metabolic Activation | Test Group | Dose level per plate | Revertant Colony Count (Mean) | ||||
TA1535 | TA1537 | TA98 | TA100 | WP2uvRA | |||
without activation | DMSO | 10 | 8 | 24 | 93 | 41 | |
Untreated | 14 | 16 | 21 | 105 | 41 | ||
MMP | 3 µg | 10 | 7 | 18 | 93 | 38 | |
10 µg | 8 | 9 | 23 | 100 | 39 | ||
33 µg | 12 | 10 | 27 | 98 | 35 | ||
100 µg | 13 | 10 | 27 | 105 | 42 | ||
333 µg | 14 | 8 | 30 | 120 | 44 | ||
1000 µg | 13 | 4 | 16 | 116 | 51 | ||
2500 µg | 3 | 1 | 7 | 2 | 6 | ||
5000 µg | 0P | 0P | 5 | 0P | 0P | ||
NaN3 | 10 µg | 1302 | 2050 | ||||
4-NOPD | 10 µg | 538 | |||||
4-NOPD | 50 µg | 119 | |||||
MMS | 2.0 µL | 736 | |||||
with activation | DMSO | 13 | 13 | 30 | 94 | 45 | |
Untreated | 12 | 15 | 40 | 90 | 48 | ||
MMP | 3 µg | 17 | 17 | 32 | 95 | 49 | |
10 µg | 14 | 11 | 30 | 101 | 44 | ||
33 µg | 13 | 13 | 27 | 94 | 54 | ||
100 µg | 15 | 12 | 32 | 103 | 53 | ||
333 µg | 12 | 12 | 29 | 93 | 41 | ||
1000 µg | 15 | 14 | 35 | 90 | 43 | ||
2500 µg | 9 | 11 | 3 | 6 | 46 | ||
5000 µg | 0P | 0P | 0P | 0P | 0P | ||
2-AA | 2.5 µg | 305 | 349 | 3950 | 2831 | ||
2-AA | 10.0 µg | 225 |
P: Precipitate, NaN3: sodium azide, 2 -AA: 2- aminoanthracene, 4 -NOPD: 4 -nitro-o-phenylene-diamine, MMS: methyl methane sulfonate
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: negative with and without metabolic activation, no mutagenic potential was detected in the bacterial reverse mutation assay.
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