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Diss Factsheets
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EC number: 247-955-1 | CAS number: 26748-41-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2011-08-04 to 2011-10-05
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted 2010
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- adopted 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.6800 (Modified Activated Sludge, Respiration Inhibition Test for Sparingly Soluble Chemicals)
- Version / remarks:
- adopted 1996
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: One test solution with a final volume of 300 mL was tested per treatment in a glass flask. 9.6 mL synthetic sewage and an adequate volume of the stock solution of the reference item were filled up with water (deionised water) to 150 mL before the start of the test. At the test item treatments 9.6 mL synthetic sewage was filled up with water to 150 mL before the start of the test. The adequate amount of the test item was weighted in a small inert plastic dish (plate). At the start of the test 150 mL activated sludge inoculum with a sludge concentration of 3 g/L (on dry weight basis) was added, first to the first blank control (CBA, thereafter CBB and CBC the “start” CB group), then in appropriate time intervals to the nitrification controls, the test solutions of the reference item and the test item (the small inert plastic dish containing the adequate amount of the test item was added shortly before the inoculum addition) and finally to the sixth blank control (CBD, after the CBE and CBF the “end” CB group). - Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Preparation of inoculum for exposure: The coarse particles were removed by settling for 10 minutes, and the upper layer of finer solids was decanted. The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in isotonic saline solution with shaking and again centrifuged. This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to dry weight determined. Based on this ratio, calculated amount of wet sludge was suspended in isotonic saline solution to yield a concentration equivalent to 3 g per litre (on dry weight basis). The activated sludge was not used on the day of the collection but continuously aerated (~2L/minute) at the test temperature for about 24 hours (1 day) and was fed once with 50 mL synthetic sewage/L activated sludge. The pH of the activated sludge inoculum was checked after preparation and before use. The pH of the activated sludge inoculum after preparation was 7.69, before use: 7.71. The pH adjustment before use was considered as not necessary.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 19.1-22.0 °C
- pH:
- The pH of the activated sludge inoculum after preparation was 7.69, before use: 7.71. The pH adjustment before use was considered as not necessary.
- Nominal and measured concentrations:
- 130, 216, 360, 600 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer bottles of approximately 300 mL volume
- Aeration: With compressed air (0.5 litre per minute)
- No. of organisms per vessel: 3 g/L activated sludge based on dry weight
- No. of vessels per concentration (replicates): five replicates
- No. of vessels per control (replicates): six blank controls, three nitrification controls, three refernce controls
TEST MEDIUM / WATER PARAMETERS
- in accordance to the guideline
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED:
respiration rate after 3 hours - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 966.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2 053.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- An additional nitrification control was examined in the main test with three parallels to check the possible nitrification potential of the applied activated sludge batch. With the applying of the nitrification control the differentiation between the total, heterotrophic and nitrification respiration was possible. The total respiration (RT) was 67.41 mg/Lh, the heterotrophic respiration (RH) was 66.79 mg/Lh, the nitrification respiration (RN): 0.62 mg/Lh was calculated according to the following equation: RN= RT-RH. The obtained 0.62 mg/Lh was insignificant [lower than the 5 % of RT (3.37 mg/Lh) in blank controls]. According to the above calculations it was assumed that the heterotrophic oxygen uptake equals the total uptake.
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: The 3-hour EC50 of 3,5-Dichlorophenol was calculated to be 6.10 mg/L, (95 % confidence limits: 4.91-7.59). - Validity criteria fulfilled:
- yes
- Conclusions:
- The EC10 was calculated to be 966.9 mg/L and the EC50 was calculated to be > 1000 mg/L.
- Executive summary:
The purpose of the 3-hour test was to evaluate the influence of the test item TBPND on the activity of activated sludge by measuring the respiration rate under defined conditions. The respiration rates (total, heterotrophic and nitrification oxygen uptake rates) of samples of activated sludge fed with synthetic sewage were measured in an enclosed cell containing an oxygen electrode after a contact time of 3 hours. Following concentraions were tested 130, 216, 360, 600 and 1000 mg/L. At the concentrations of 130, 216 and 360 mg/L the observed-calculated specific respiration rates were within the range of the specific respiration rates of the blank controls, and within the biological variability of the applied system. The occurring negative values were considered and evaluated as zero inhibition. The inhibition of the specific respiration rates compared to the respiration rates of the blank controls was 3.62 % at 130 mg/L, -0.31 % at 216 mg/L and -0.24 % at 360 mg/L. The test item showed slight inhibitory effect at the concentrations of 600 mg/L (7.70 %) and at 1000 mg/L (10.23 %). An EC10 of 966.9 mg/L was detected. The EC50 was calculated to be 2053.9 mg/L.
Reference
Description of key information
The toxicity to microorganisms was assessed according to OECD guideline 209, EU-method C.11 and EPS OPPTS 850.6800. After 3 hours of exposure an EC10 of 966.9 mg/L was detected.The EC50 was calculated to be 2053.9 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 2 053.9 mg/L
- EC10 or NOEC for microorganisms:
- 966.9 mg/L
Additional information
The purpose of the 3-hour test was to evaluate the influence of the test item TBPND on the activity of activated sludge by measuring the respiration rate under defined conditions. The respiration rates (total, heterotrophic and nitrification oxygen uptake rates) of samples of activated sludge fed with synthetic sewage were measured in an enclosed cell containing an oxygen electrode after a contact time of 3 hours. Following concentraions were tested 130, 216, 360, 600 and 1000 mg/L. At the concentrations of 130, 216 and 360 mg/L the observed-calculated specific respiration rates were within the range of the specific respiration rates of the blank controls, and within the biological variability of the applied system. The occurring negative values were considered and evaluated as zero inhibition. The inhibition of the specific respiration rates compared to the respiration rates of the blank controls was 3.62 % at 130 mg/L, -0.31 % at 216 mg/L and -0.24 % at 360 mg/L. The test item showed slight inhibitory effect at the concentrations of 600 mg/L (7.70 %) and at 1000 mg/L (10.23 %). An EC10 of 966.9 mg/L was detected. The EC50 was calculated to be 2053.9 mg/L.
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