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EC number: 406-940-1 | CAS number: 126019-82-7 DP 211
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in soil
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in soil: simulation testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 307 (Aerobic and Anaerobic Transformation in Soil)
- GLP compliance:
- no
- Test type:
- laboratory
- Specific details on test material used for the study:
- - 4 nonlabeled and branched 4-NP isomers: 4-NP111, 4-NP112, 4-NP38 and 4-NP65 (see structures attached)
- 2 isotope-labeled isomers (14C-4-NP111, 13C-4-NP38) synthesized using the Friedel-Crafts alkylation - Radiolabelling:
- yes
- Oxygen conditions:
- aerobic
- Soil classification:
- not specified
- Year:
- 2 011
- Soil no.:
- #1
- Soil type:
- other: gleyic hydragric anthrosol
- % Clay:
- 46.7
- % Silt:
- 37.9
- % Sand:
- 15.4
- % Org. C:
- 2.5
- pH:
- 6.31
- Details on soil characteristics:
- SOIL COLLECTION AND STORAGE
- Geographic location: rice paddy soil collected from the Changshu Experimental Station of the Chinese Academy of Sciences in Jiangsu Province, China
- Collection procedures: not stated
- Soil preparation (e.g., 2 mm sieved; air dried etc.): The soil was air dried, sieved through 2 mm and stored at room temperature shortly before use. - Soil No.:
- #1
- Duration:
- 58 d
- Soil No.:
- #1
- Initial conc.:
- 59.6 other: µmol/kg soil dw
- Based on:
- other: mixture of 5 nonylphenol isomers (4 branched and 1 linear)
- Soil No.:
- #1
- Initial conc.:
- ca. 12 other: µmol/kg soil dw
- Based on:
- other: of each isomer
- Soil No.:
- #1
- Initial conc.:
- 3.7 other: MBq/kg soil dw
- Based on:
- other: mixture of 5 nonylphenol isomers (4 branched and 1 linear)
- Parameter followed for biodegradation estimation:
- CO2 evolution
- radiochem. meas.
- Soil No.:
- #1
- Temp.:
- 20 ± 1 °C
- Humidity:
- 70% of the max water-holding capacity
- Details on experimental conditions:
- EXPERIMENTAL DESIGN
- Soil condition: air dried
- Soil (g/replicate): 5 g
- No. of replication treatments: 54
- Test apparatus (Type/material/volume): Serum flask
- Details of traps for CO2 and organic volatile, if any: The 14CO2 released from the soil was absorbed by 1.0 mL of NaOH (1 M) contained in one 6mL vial, which was suspended from the bottom of the stopper.
Test material application
- Volume of test solution used/treatment: A stock solution of a mixture of five 4-NP isomers (14C-4- NP111, 4-NP112, 13C-4-NP38, 4-NP65, and 4-NP1) was prepared in methanol at a concentration of 16.6 μmol/mL with a molar ratio of the five isomers in the mixture at about 1:1:1:1:1 (each isomer at about 3.3 mmol/L). About 18 μL of the stock solution of the 4-NP isomer mixture was added with a microsyringe to 0.2 g of soil.
- Application method (e.g. applied on surface, homogeneous mixing etc.): The soil was mixed and transferred into a 100 mL serum flask containing 4.8 g of soil. The whole soil was then thoroughly mixed and kept overnight to evaporate the methanol solvent. The homogeneity of the 4-NP distribution within the soil was proved by determining the radioactivity of soil subsamples (0.02-0.05 g) from the flask. After solvent evaporation, 1.2 mL distilled water was added to adjust the soil moisture to 70% of the max WHC. The flask was then closed with a rubber stopper.
3. OXYGEN CONDITIONS
- Methods used to create the aerobic conditions: The flasks were opened for 0.5 min each day for exchange of head-space with fresh air.
- Is the co-solvent evaporated: yes
Experimental conditions (in addition to defined fields):
- Continuous darkness: yes
4. SUPPLEMENTARY EXPERIMENTS: Preliminary experiments showed that the applied consecutive extractions with methanol and ethyl acetate was sufficient and exhaustive. Radioactive determinations showed that the freeze-drying and extraction processes had a recovery of 93.1 +- 1.5% for 4-NP isomers.
5. SAMPLING DETAILS
- Sampling intervals: At incubation times of 0, 5, 10, 15, 20, 27, 34, 43 and 58 days, three flasks were sacrificed for analysis of radioactivity in the NaOH trap, concentration of the 4-NP isomers and formation of metabolites and bound residues of 14C-NP111 in the soil.
- Sampling method for soil samples:
> Sterility check, if sterile controls are used: yes, soil sterilization was achieved by autoclavingthe soils at 120 °C for 1h three times in three consecutive days.
> Moisture content: 70% max WHC - Soil No.:
- #1
- % Total extractable:
- 38.3
- % Non extractable:
- 54.4
- % CO2:
- 5
- % Other volatiles:
- 0
- % Recovery:
- 97.7
- Remarks on result:
- other: Test substance: 14C-NP111
- Parent/product:
- parent
- Key result
- Soil No.:
- #1
- % Degr.:
- ca. 5
- Parameter:
- CO2 evolution
- Sampling time:
- 58 d
- Remarks on result:
- other: 14C-NP-111
- Key result
- Soil No.:
- #1
- DT50:
- > 60 d
- Temp.:
- 20 °C
- Remarks on result:
- other: 14C-NP111, based on mineralisation, extrapolated from study results
- Key result
- Soil No.:
- #1
- DT50:
- > 2.1 - < 10.3 d
- Type:
- (pseudo-)first order (= half-life)
- Temp.:
- 20 °C
- Remarks on result:
- other: mean DT50 of the 4 branched isomers; DT50 is mainly based on disappearance due to adsorption (NER) and incorporation in the soil humic acid matrix
- Transformation products:
- not specified
- Details on transformation products:
- One metabolite of 14C-4-NP111 was found in the active soil, but no metabolite was detected in the sterilized soil. The metabolite had a higher Rf value (0.75) on TLC than that of the parent 14C-4-NP111 (0.38), indicating that the metabolite was less polar than 4-NP111.
- Evaporation of parent compound:
- no
- Volatile metabolites:
- no
- Residues:
- yes
- Details on results:
- TEST CONDITIONS
- Aerobicity, moisture, temperature and other experimental conditions maintained throughout the study: Yes
- Anomalies or problems encountered (if yes): no
NON-EXTRACTABLE RESIDUES
- % of applied amount at end of study period: ~ 54% (14C-NP111) -> 31.1% after 5 days
MINERALISATION
- % of applied radioactivity present as CO2 at end of study: about 5% (14C-NP111)
VOLATILIZATION
- % of the applied radioactivity present as volatile organics at end of study: negligible
STERILE TREATMENTS (if used)
- Transformation of the parent compound: <5% mineralized
- Formation of transformation products: no
- Formation of extractable and non-extractable residues: yes
- Volatilization: no - Conclusions:
- Branched nonylphenol quickly diseapper in soil by sorption to and incorporation into the soil matrix. However, mineralisation to CO2 is slow (~ 5% in 58 days).
- Executive summary:
Using 14C- and 13C-ring-labeling, degradation of five p-nonylphenol (4-NP) isomers including four branched (4-NP38, 4-NP65, 4-NP111, and 4-NP112) and one linear (4-NP1) isomers in a rice paddy soil was studied under oxic conditions. Mineralisation showed to be in the range of 5%. Over 50% of the parent compound disappeared as NER after 58 days. Thus, branched NP isomers disappear quickly in soil due to sorption and diffusion and form NERs, but mineralisation is low.
- Endpoint:
- biodegradation in soil: simulation testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Justification for type of information:
- Read across justification document is attached in chapter 13.
- Reason / purpose for cross-reference:
- read-across source
- Soil No.:
- #1
- % Total extractable:
- 38.3
- % Non extractable:
- 54.4
- % CO2:
- 5
- % Other volatiles:
- 0
- % Recovery:
- 97.7
- Remarks on result:
- other: 14C-NP111; day 56
- Parent/product:
- parent
- Key result
- Soil No.:
- #1
- % Degr.:
- 5
- Parameter:
- CO2 evolution
- Sampling time:
- 56 d
- Remarks on result:
- other: 14C-NP111
- Key result
- DT50:
- > 60 d
- Temp.:
- 20 °C
- Remarks on result:
- other: 14C-NP111, based on mineralisation
- Transformation products:
- not specified
Referenceopen allclose all
Isomer-Specific Degradation of 4-NP Isomers in Soil. The five (four branched and one linear) 4-NP isomers degraded at different rates in the active rice paddy soil during 58 days of incubation under oxic conditions. The degradation kinetics of the isomers was fitted to the availability-adjusted firstorder
model, the first-order model with one constant parameter, and the simple first-order model. Among the three models, the availability-adjusted first-order model had the best goodness-of-fit and is most rational for describing the gradually increasing unavailability of 4-NP isomers in soil over incubation time. Also, the residual plots of this model show a random distribution of the residues around the
zero line. Therefore, we prefer to apply the availability-adjusted model to fit the
degradation data of the five 4-NP isomers in order to compare their persistence in the soil.
The values of parameters k, a, and t1/2 as well as the goodness-of-fit of the model for the The k ranged from 0.09 to 0.58 per day with the following increasing order: 4-NP1 (0.58 day-1) > 4-NP38 (0.38 day-1) > 4-NP65
(0.13 day-1) > 4-NP112 (0.10 day-1) > 4-NP111 (0.09 day-1), showing that the linear isomer 4-NP1 disappearing most rapidly in the soil (t1/2 = 1.4 days), while the branched 4-NP111, the main component of tNP mixtures,15 disappeared considerably more slowly (t1/2 = 10.3 days). a varied from 0.04 to 0.20 day-1, and 4-NP1 had the highest unavailability factor, which is in good agreement with its highest sorption affinity in soils. The unavailable amounts of the five isomers in the soil ranged from 2.9 to 24.4% of the initial spiking with the following decreasing order: 4-NP111 (24.4%) > 4-NP112 (7.9%) > 4-NP1 (5.6%) > 4-NP65 (3.3%) > 4-NP38 (2.9%). 4-NP111 and 4-NP112, which were more recalcitrant, also had higher unavailable percentage in the soil.
By using defined 4-NP isomers, we clearly showed that the degradation of 4-NP isomers in soil was isomer specific.
The observed higher recalcitrance of the branched 4-NP isomers than 4-NP1 can be attributed to the alkyl chain structure at the benzene ring. The branched isomers have a quaternary a-C on the alkyl chain, and this structure is regarded resistant to .- and ß-oxidation. The length of the side chain at a-C seems to be the most important factor for their degradation.
The three isomers with an ethyl side chain at a-C (4-NP111, 4-NP112, 4-NP65) showed a longer t1/2 than 4-NP38 with two methyl side chains at a-C.
Description of key information
The substance is considered to be persistent in soil
Key value for chemical safety assessment
Additional information
- A biodegradation in water and sediment simulation test according to OECD 308 shows that O,O,O-tris(2(or 4)-C9-10-isoalkylphenyl) phosphorothioate quickly adsorb to sediment. This is supported by experimental data on adsorption (log Koc = 3.7 -4.7, see study in chapter 5.4.1). The same rapid adsorption processes will surely also happen (even to a higher degree) in soil.
- The simulation test further shows that the test substance neither mineralize in the sediment (0.1-0.3% in 100 days) nor form high amounts of metabolites. A maximum of 4.0 % Nonylphenol and 4.3 % Tris(nonylphenol)phosphate were detected. The amounts were even lower at the end of the study.
- The given supporting study clearly shows that even this branched nonylphenol will not mineralize rapidly.
The substance was found to be very persistent in water/sediment systems in a study according to OECD 308 (BASF 2021). In accordance with ECHA "Guidance on Information Requirements and Chemical Safety Assessment Chapter R.11: PBT/vPvB assessment", Section R.11.4.1.1 (ECHA, version 3.0, June 2017) if a substance or its degradation products are concluded to be persistent or very persistent in one environmental compartment, there is no need for further testing for persistence assessment in remaining compartments.
Since no simulation studies assessing the biodegradability of O,O,O-tris(2(or 4)-C9-10-isoalkylphenyl) phosphorothioate (CAS 126019-82-7) in soil are available, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5 Grouping of substances, a read-across to Phenol,4-nonyl-,branched (CAS 84852-15-3) was conducted. The read across is justified by Phenol,4-nonyl-,branched (CAS 84852-15-3) being an impurity and a degradation product of the test substance with the highest degradation potential (see table and Analogue justification document attached).
In the supporting study, Shan et al. (2011) studied the degradation of five p-nonylphenol (4-NP) isomers including four branched (4-NP38, 4-NP65, 4-NP111, and 4-NP112) and one linear (4-NP1) isomers in a rice paddy soil using unlabeled as well as 14C- and 13C-ring-labelled test material. The test design was similar to OECD guideline 307. Test substances were incubated up to 56 days at 20 °C. Extractable residues, non-extractable residues as well as CO2 was measured after certain time intervals. Degradation (= mineralization + NERs) followed availability-adjusted first-order kinetics with the decreasing order of half-life 4-NP111 (10.3 days) > 4-NP112 (8.4 days) > 4-NP65 (5.8 days) > 4-NP38 (2.1 days) > 4-NP1 (1.4 days). One metabolite of 4-NP111 with less polarity than the parent compound occurred rapidly and remained stable in the soil. At the end of incubation (56 days), bound residues of 4-NP111 amounted to 54% of the initially applied radioactivity and resided almost exclusively in the humin fraction of soil organic matter, in which chemically humin-bound residues increased over incubation. Mineralization (as CO2 formation) was measured to be 5% after 56 days for the branched 14C-NP111. Thus, branched nonylphenol is mineralized slowly but rapidly integrated into organic matter (humin fraction) in soil.
Together with additional experimental data and physico-chemical properties of the test substance given in the respective chapters of the dossier one can surely conclude that O,O,O-tris(2(or 4)-C9-10-isoalkylphenyl) phosphorothioate is persistent in soil:
Thus, O,O,O-tris(2(or 4)-C9-10-isoalkylphenyl) phosphorothioate can surely be judged persistent in soil based on the existing data for the substance itself and a suitable source substance and further simulation testing in soil with the test substance would not generate relevant new data for the substance to register.
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