1H-Pyrazole-5-carboxylic acid, 3-bromo-1-(3-chloro-2-pyridinyl)-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

March 28, 2006-April 1, 2006

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented, followed GLP and OECD guideline 203.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Remarks:

The study was conducted according to the guideline in effect at time of study conduct.

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Remarks:

The study was conducted according to the guideline in effect at time of study conduct.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

481-150-8

EC Name:

-

Cas Number:

500011-86-9

Molecular formula:

C9H5BrClN3O2

IUPAC Name:

3-bromo-1-(3-chloropyridin-2-yl)-1H-pyrazole-5-carboxylic acid

Constituent 2

Reference substance name:

DBC80

IUPAC Name:

DBC80

Constituent 3

Reference substance name:

1H-Pyrazole-5-Carboxylic Acid, 3-Bromo-1-(3-Chloro-2-Pyridinyl)-

IUPAC Name:

1H-Pyrazole-5-Carboxylic Acid, 3-Bromo-1-(3-Chloro-2-Pyridinyl)-

Details on test material:

- Name of test material (as cited in study report): IN-DBC80
- Physical state: Tan powder
- Analytical purity: 96.7%
- Lot/batch No.: 008
- Expiration date of the lot/batch: July 22, 2008
- Stability under test conditions: Stable
- Storage condition of test material: In the dark, at room temperature.
- Source: E.I. DuPont De Nemours and Company

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Controls and 120 mg/L
- Sampling method: Not specified, collected in glass.
- Sample storage conditions before analysis: Not specified

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls: Dilution water and Vehicle control
- Chemical name of vehicle: Acetone
- Concentration of vehicle in test medium: < 0.1 mL/L
- Evidence of undissolved material: None

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: Old India Aquarists, Chennai, India
- Length at study initiation: 3.02 ± 0.013 cm
- Weight at study initiation: 0.239 ± 0.014 g
- Feeding during test: No

ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions: Yes
- Type and amount of food: Commercial fish food
- Feeding frequency: once daily
- Health during acclimation (any mortality observed): None

QUARANTINE
- Duration: 12 days
- Health/mortality: No mortality

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

None

Test conditions

Hardness:

212-216 mg/L as CaCO3

Test temperature:

22 ± 2° Celcius

pH:

7.82-8.41

Dissolved oxygen:

7.62-7.81 mg/L

Salinity:

Not applicable

Nominal and measured concentrations:

Control, solvent control, 120 mg/L test solution.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: Glass aquaria, 10 L solution
- Renewal rate of test solution: None
- No. of organisms per vessel: 7
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- No. of vessels per vehicle control: 1
- Biomass loading rate: < 1 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 1: 1 mixture of hard and soft water.
- Conductivity: 1162-1196 µ siemans/cm
- Culture medium different from test medium: No
- Intervals of water quality measurement: 24 hours

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 12 hours light, 12 hours dark.
- Light intensity: Artificial fluorescent

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality, at 3, 6, 24, 48, and 96 hours.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: Limit test- 1 concentration only.
- Range finding study concentrations: 7.5, 15, 30, 60, and 120 mg/L
- Results used to determine the conditions for the definitive study: Yes

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: No mortality or sublethal effects observed.

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Reported statistics and error estimates:

No statistical analyses performed.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96 h LC50 of IN-DBC80 to B.rerio is > 120 mg/L.