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EC number: 229-761-9 | CAS number: 6711-48-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-02-29 - 2012-03-03
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Bis-DMAPA
- Physical state: liquid
- Supplier: SGS Belgium N.V.
- Batch number: PFW110019
- Purity: 97.8%
- Purity test date: May 30th 2011
- Total amine content: 16.02 meq/g
- Storage condition of test material: lab storage - Analytical monitoring:
- yes
- Details on sampling:
- - Samples for test concentration analysis were taken at 0 and 48 h from all test solutions (at the start of the test excess test solutions were sampled and at the end of the test 1 replicate of each test solution was sampled).
- Sampling method: not specified
- Sample storage conditions before analysis: not specified - Vehicle:
- no
- Details on test solutions:
- - Controls: dilution water
- A stock concentrate of Bis-DMAPA with a nominal concentration of 120 mg/L was prepared by adding a nominal 0.24 g of test substance to 2000 mL of dilution water. The concentrate was inverted several times, which resulted in a clear and colourless solution. This stock concentrate was then used to prepare the test solutions. This was achieved by the direct addition of the appropriate amount of concentrate to 1000 mL of dilution water in a volumetric flask. For the highest exposure concentration the concentrate was used without any further dilution. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: in-house culture
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: diploid parthenogenesis
- Feeding during test: no
ACCLIMATION
- Acclimation period: none
- Acclimation conditions (same as test or not): reared in same conditions as test
- Type and amount of food: a mixed algae diet of Chlorella vulgaris, strain CCAP 211/12 and Pseudokirchneriella subcapitata, strain CCAP 278/4, supplied ad libitum
- Feeding frequency: depending on age and density of the culture
- Health during acclimation: test organisms and culture from which they were obtained showed no evidence of disease before the test period - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
- Hardness:
- 222.3 mg CaCO3/L
- Test temperature:
- 20 ± 1°C
- pH:
- Start of test: 8.2-8.8
End of test: 7.8-8.3 - Dissolved oxygen:
- Start of test: 7.5-8.2 mg/L
End of test: 8.2-9.1 mg/L - Salinity:
- N/A
- Nominal and measured concentrations:
- Nominal exposure concentrations: control, 5, 11, 25, 55, 120 mg/L.
The mean measured concentrations ranged from 100-117% of nominal. - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beaker
- Type (delete if not applicable): covered with loose fitting glass lid
- Material, size, headspace, fill volume: glass, 250 mL, 200 mL fill volume
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water medium - Elendt's M4 D. magna medium
- Total organic carbon: < 1 mg/L
- Particulate matter: < 3 mg/L
- Pesticides: < 0.1 µg/L
- Alkalinity: 39.6 mg CaCO3/L
- Conductivity: 608 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: by batch
OTHER TEST CONDITIONS
- Adjustment of pH: with 1M HCl to suitable range
- Photoperiod: 16 hours light:8 hours dark, with 20 minute transition periods
- Light intensity: normal ambient
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality, 24 and 48 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2 - Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 50.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI = 41.1-63.7 mg/L
- Details on results:
- - Behavioral abnormalities: none
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: none
- 24-h EC50 = 53.3 mg/L (95% CI = 43.4-67.9 mg/L)
- 48-h NOEC = 25 mg/L
- 48-h LOEC = 55 mg/L - Reported statistics and error estimates:
- The 24 and 48 hour EC50s were calculated using the moving average angle method.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study, performed according to OECD guideline 202, the 48-h EC50 of the test substance for the freshwater invertebrate Daphnia magna was calculated to be 50.3 mg/L. Test concentrations were verified at the start and end of testing and agreed with nominal concentrations. The results of this well performed study can be considered reliable.
Reference
Description of key information
One study (Fox et al., 2012) is considered as the key study for endpoint coverage. The study investigates the acute toxicity of the substance to Daphnia magna according to the OECD guideline 202 under static conditions. The 48h-EC50 was determined to be 50.3 mg test material/L (based on nominal concentrations). The study is given a Klimisch score of 1 and was conducted under GLP.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 50.3 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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