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Diss Factsheets
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EC number: 200-238-7 | CAS number: 55-56-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Additional toxicological data
Administrative data
- Endpoint:
- additional toxicological information
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1986
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Antiseptic toxicity in wounds healing by secondary intention
- Author:
- Brennan SS, Foster ME & Leaper DJ
- Year:
- 1 986
- Bibliographic source:
- J Hosp Infect 8, 263-267
Materials and methods
- Type of study / information:
- Effects on chronic ulcer care and biochemical and histological parameters have been examined
- Principles of method if other than guideline:
- In 135 Sprague-Dawley female rats (90-110 g) the skin over the thoraco-lumbar spine was removed with hair clippers. Four 10 mm full thickness skin wounds were made approximately 1 cm apart in the shaved area.
Controls (N=45): Normal saline was applied to each wound using copolymer T-starch hydrogel as a vehicle.
Chlorhexidine (N=45): Aqueous 0.05 % chlorhexidine was applied in place of normal saline.
The vehicle used is able to absorb up to 130 % of its weight of fluid and is chemically inert. One gram of dry granules were saturated with 5 ml of saline or antiseptic agent, resulting in the formation of a thick paste. One mL of the paste was applied to each wound and all four wounds were then covered with a polyacrylamide dressing and an adhesive polyurethene film, which were kept in place with a secondary elastoplast dressing.
Rats were housed individually. On the third, fifth and seventh postoperative days, 15 rats from each group were killed and the granulation tissue, which constituted the healing wounds, was excised from two of the wounds of each animal and stored at -20°C prior to biochemical analysis. The two remaining wounds were excised with a rim of skin and placed in formol saline for histological analysis and oculometric analysis of re-epithelialisation. Granulation tissue from one wound was assayed for hydroxyproline content, as a measure of collagen synthesis, and from the other for deoxyribonucleic acid (DNA), as a measure of tissue cellularity.
The Mann-Whitney U Test has been used for statistical analysis.
Test material
- Reference substance name:
- D-gluconic acid, compound with N,N''-bis(4-chlorophenyl)-3,12-diimino-2,4,11,13-tetraazatetradecanediamidine (2:1)
- EC Number:
- 242-354-0
- EC Name:
- D-gluconic acid, compound with N,N''-bis(4-chlorophenyl)-3,12-diimino-2,4,11,13-tetraazatetradecanediamidine (2:1)
- Cas Number:
- 18472-51-0
- IUPAC Name:
- N',N'''''-hexane-1,6-diylbis[N-(4-chlorophenyl)(imidodicarbonimidic diamide)] - D-gluconic acid (1:2)
Constituent 1
Results and discussion
Applicant's summary and conclusion
- Executive summary:
Compared to controls, no effect of chlorhexidine application to wounds were noted when the following parameters were assessed: collagen formation in granulation tissue as determied by hydroxyproline content, DNA-content of tissue in wounds, histological observation. Therefore, adverse effects concerning wound healing after application of chlorhexidine (used as antimicrobial agent) are not to be expected.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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