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EC number: 209-798-7 | CAS number: 593-56-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, GLP, Monitoring.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Bis(hydroxylammonium) sulphate
- EC Number:
- 233-118-8
- EC Name:
- Bis(hydroxylammonium) sulphate
- Cas Number:
- 10039-54-0
- Molecular formula:
- H3NO.1/2H2O4S
- IUPAC Name:
- bis(hydroxyammonium) sulfate
- Details on test material:
- - Analytical purity: 99.13 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: The concentration control analyses were performed with test solutions of the following nominal concentrations of the test substance: 0, 1.0, 0.5, 0.25, 0.125, 0.0625, 0.0313, 0.0156 mg/l. Samples for analysis were taken in the 1st, the 2nd and the 3rd week of the test. For each concentration the freshly prepared test solution (unstocked) and the corresponding 24h old test solution (stocked with daphnids) were analyzed.
- Sampling method/ Storage: To 50 mL test solution 3 drops 10% H2SO4 were added to stabilize the concentration. Retain samples were taken and stored in the refrigerator.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test concentrations were prepared by serial dilution of a stock solution. The stock solutions for the test intervals were prepared by dilution of 1 mg test substance in 1 L test water followed by stirring with a magnetic stirrer for about 1 hour. The stock solution appeared colorless clear.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: The clone of Daphnia magna STRAUS used was supplied by Institut National de Recherche Chimique Appliquée, France, in 1978. The daphnids are cultured under standard conditions in the laboratory of ecotoxicology, BASF AG, "Ludwigshafen".
- Age of parental stock: 2-4 weeks
- Age of the animals at the start of the test: 2 -24 h
- Culture conditions: The daphnid tests are performed under the same environmental conditions as the culture itself.
- Changing of the breeding water: Monday, Friday
- Feeding during test: Yes, Desmodesmus subspicatus, cultured in a synthetic medium, daily
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- Hardness:
- 2.20 - 3.20 mmol/L
- Test temperature:
- 20.3 - 21.2 °C
- pH:
- 7.6 - 8.3
- Dissolved oxygen:
- 5.3 - 9.4 mg/L
- Nominal and measured concentrations:
- 0, 1, 0.5, 0.25, 0.125, 0.0625, 0.0313, 0.0156 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: closed test system with numbered glass vessels with glass plugs (nominal volume approx. 50 mL)
- Fill volume: approx. 50 mL (Because of the volatility of the test substance the test vessels were closed with a glass plug and filled without headspace).
- Renewal of test solution: daily
- No. of organisms per vessel: 1 parent animal
- No. of vessels per concentration (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic medium M4
- This synthetic medium has the following properties:
Alkalinity up to pH 4.3: 0.80 - 1.00 mmol/L
Molar ratio Ca:Mg: about 4 : 1
pH value: 7.5-8.5
Conductivity: 550 - 650 uS/cm
- After preparation the M4 medium was aerated for approximately 24 h until saturation with oxygen is reached.
OTHER TEST CONDITIONS
- Photoperiod: Artificial Iight, OSRAM L58 W31 warm white; day/night-rhythm = 16 / 8 hours
- Light intensity: about 1 - 8 µE/(m2.s) at a wave length of 400 - 700 nm
Because of the volatility of the test substance the test vessels were filled without headspace and closed with glass plugs.
EFFECT PARAMETERS MEASURED
- Reproduction and mortality, daily observation intervals
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.31 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: length
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.616 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
Any other information on results incl. tables
a)
Analytically measured concentrations of the test substance in the test solution:
Nominal | 1. sampling | 2. sampling | 3. sampling | Mean | Mean | |||
Conc. | day 0 | day 1 | day 8 | day 9 | day 20 | day 21 | value* | value* |
[mg/L] | [mg/L] | [mg/L] | [mg/L] | [mg/L] | [mg/L] | [mg/L] | [mg/L] | [%] |
0 | < 0.01 | < 0.01 | < 0.01 | < 0.01 | < 0.01 | < 0.01 | < 0.01 | < 0.01 |
0.0156 | 0.017 | < 0.01 | 0.017 | < 0.01 | 0.017 | < 0.01 | 0.0085 | 54.5 |
0.0313 | 0.035 | < 0.01 | 0.033 | < 0.01 | 0.035 | < 0.01 | 0.017 | 54.8 |
0.0625 | 0.085 | < 0.01 | 0.063 | < 0.01 | < 0.01 | < 0.01 | 0.025 | 39.5 |
0.125 | 0.169 | < 0.01 | 0.128 | 0.017 | 0.140 | < 0.01 | 0.076 | 60.5 |
0.250 | 0.33 | < 0.01 | 0.250 | 0.019 | 0.263 | < 0.01 | 0.144 | 57.5 |
0.500 | 0.718 | < 0.01 | 0.552 | 0.064 | 0.516 | < 0.01 | 0.308 | 61.7 |
1.000 | 1.265 | 0.013 | 1.055 | 0.236 | 1.081 | 0.048 | 0.616 | 61.6 |
*The
mean value was calculated as arithmetic mean of the concentration at
start of the
intervals and the limit of quantification if the concentration
at the end of the intervals was below the limit of quantification.
The analytically determined concentrations of the test substance in the
test water were
in a range of 80 . 120 % of the nominal concentration for the
freshly prepared solution with the following exceptions:
- 127-144 % of the nominal value were detected in the concentrations
>=0.0625 mg/L
on day 0
- the concentration was below the detection limit on day 20 in the test
concentration
0.0625 mg/L.
At the end of the test intervals after 24 hours, the substance decomposed
almost
completely. Only in the highest test concentration, it was possible
to detect low amounts of the test substance after 24 hours.
In a preliminary stability test at a very low concentration of 10 µg/L
the
decomposition of the test substance was very fast. In higher
concentrations
the decompositions seems to be slower. The highest selected
test concentration in this study was 1 mg/L based on the results
of an acute
study.
b) Biological results:
Nominal concentrations:
Reproduction/ Weight/
Length
NOEC [mg/L] >=
1/ >= 1/ 0.5
LOEC [mg/L] >
1/ > 1/ 1
Measured concentrations:
Reproduction/
Weight/ Length
NOEC [mg/L] >=0.616/
>= 0.616/ 0.308
LOEC [mg/L] >
0.616/ > 0.616/
0.616
Summary of the LC0 (mortality of the parent animals) after 21 days
LC0 >= 1 mg/L (nominal)
LC0 >= 0.616 mg/L (measured)
Since the toxicity observed in this test may be caused by the instable
test substance
and/or by its degradation products and since the half life period
is very short, the effect concentration, which is based on the
nominal
concentrations, should be preferably used for the evaluation of
the test
substance.
c) Water parameter:
Minimum and maximum values of the chemical and physical characteristics of the test solutions during the test period.
Parameter Minimum Maximum
--------------------------------------------------------------------------------------------
pH value 7.6 8.3
Oxygen content [mg/L] 5.3 9.4
Temperature [°C]* 20.3 21.2
--------------------------------------------------------------------------------------------
* measured in a separate vessel close to the test vessels
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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