Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 288-315-1 | CAS number: 85711-55-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Four samples were taken from the freshly prepared control and test media at 0 and 72 hours, respectively. Four samples were taken from the expired control and test media at 24 and 96 hours.
Samples were stored frozen until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: water accommodated fraction (WAF)
The test material was dispersed in dilution water in a glass aspirator at a nominal concentration of 100 mg/L. The contents were stirred for approximately 24 hours in the dark and then left to stand for approximately 24 hours in the dark. An aliquot was then removed from the mid-vessel region to provide the Water Accommodated Fraction (WAF). - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout
- Source:commercial fish farm in the UK
- Length at study initiation: 5.47 cm (mean total length)
- Weight at study initiation: 1.53 g (mean wet weight)
- Feeding during test: none
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): same
- Type and amount of food: commercial fish food (TROUW (UK) Ltd. Nutra Fry 02); amount equivalent to 1% of the total wet-weight of fish in the holding tank; no food was given during the 24-hour period immediately before exposure.
- Health during acclimation: no madication, no mortality - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 169 mg/L as CaCO3
- Test temperature:
- 14 -15 °C
- pH:
- 7.8-8.5
- Dissolved oxygen:
- 100-108% air saturation value
- Nominal and measured concentrations:
- nominal loading rate: 100 mg test material/L as WAF
mean measured TOC levels (after background correction):
in fresh media: 6.0-9.4 mg C/L (control mean: 0.9 mg C/L)
in expired media (24 h): 8.3-11.7 mg C/L (control mean: 1.0-1.6 mg C/L)
overall geometric mean value: 8.3 mg C/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass aquaria, containing 20 litres of medium to a depth of 18.5 cm.
- Type: open
- Aeration: yes
- Renewal rate of test solution: daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.765 g body weight/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: laboratory tap water, dechlorinated and softened by passage through a water purification system consisting of activated carbon filter, water softener and reverse osmosis treatment.
Total organic carbon: 0.9 mg C/L
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light : 8 hours dark with periods of subdued lighting at the beginning and end of each light phase
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality and sub-lethal effects; recorded at approx. 2, 4, 24, 48, 72 and 96 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable (limit test)
- Range finding study
- Test concentrations: nominal loading rates 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: no mortality or sub-lethal effects were noted after 96 hours - Reference substance (positive control):
- not required
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: results are expressed in terms of loading rates
- Duration:
- 96 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks:
- and sub-lethal effects
- Remarks on result:
- other: results are expressed in terms of loading rates
- Details on results:
- - Other biological observations: At the nominal loading rate of 100 mg/L a single mortality was recorded at 72 hours and sub-lethal effects were observed from 24 hours onwards, comprising darkening pigmentation and/or darkened eye-orbit area. These effects were attributed to the presence of a fish exhibiting aggressive behaviour, which following its isolation within the test vessel at 72 hours, the majority of fish appeared to recover (see Table 1). The absence of any mortality or sub lethal effects in the range finding test supports the conclusion that none of the effects seen in the test vessel during the definitive test were treatment-related.
- Mortality of control: zero
- Other adverse effects control: none
The test medium was colourless. - Reported statistics and error estimates:
- The “no observed effect loading rate” (NOELR) was derived by direct inspection of the data for lethal and sub-lethal effects. An incidence rate of more than 10% was considered to be significant.
- Sublethal observations / clinical signs:
Table 1 Mortality and sub-lethal effects
Loading rate (mg/L WAF)
Abnormality
Initial population = 10 fish / concentration
2h
4h
24h
48 h
72 h
96 h
Control
NAD
10/10
10/10
10/10
10/10
10/10
10/10
100***
NAD
10/10
10/10
6/10
5/10
4/9
7/9
DE
-
-
4/10*
2/10*
3/9*
-
DKE
-
-
-
3/10*
1/9*
2/9*
A
-
-
-
-
1/9**
-
NAD
no abnormalities detected
DE
darkened eye-orbital area
DKE
dark body and eye orbital area
A
aggressive behaviour; actively swimming and pursuing other fish in the vessel
*
Effects were attributed to the presence of a fish exhibiting aggressive behaviour, which following its isolation at 72 hours, the majority of the fish appeared to recover.
**
Isolation of aggressive fish
***
A single mortality was recorded at 72 hours. This death was attributed to the presence of a fish exhibiting aggressive behavior.
- Validity criteria fulfilled:
- yes
- Remarks:
- mortality of control animals <10%; concentration of dissolved oxygen in all test vessels >60% saturation
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Remarks:
- study conducted for 48 h instead of 96 h
- Qualifier:
- according to guideline
- Guideline:
- other: DIN guideline 38412, part 15
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- Quality Assurance Programme/GLP standards not specified
- Analytical monitoring:
- yes
- Details on sampling:
- Initial exposure levels were measured in the control and in the test solution.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: water accommodated fraction (WAF)
A saturated solution (initial weight: 150 mg/l) was prepared in reconstituted water and was stirred overnight. After a filtration step over a folded paper filter (595 1/2; diameter 320 mm, Schleier & Schuell, D-37582 Dassel) the filtrate was used without further treatment. - Test organisms (species):
- Leuciscus idus
- Details on test organisms:
- TEST ORGANISM
- Common name: Golden Orfe
- Source: fish farm Eggers, Hohenwestedt, Germany
- Length at study initiation: 5-8 cm
- Corpulence factor: 0.8-1.1 g/cm3
- Feeding: Tetra Menü Spezialfutter
- Feeding during test: fish were not fed during the test
ACCLIMATION
- Acclimation period: at least 7 days
- Acclimation conditions: 20 - 21 °C
- Diluent water: tap water of Karlsruhe with the following quality:
acid capacity KS(4.3): 4.75 - 4.77 mmol/L
water hardness: 2.83 - 3.14 mmol/L
calcium Ca2+: 2.41 - 2.60 mmol/L
magnesium Mg2+: 0.42 - 0.54 mmol/L
sodium Na+: 0.28 - 0.33 mmol/L
potassium K+: 0.05 - 0.06 mmol/L
pH-value: 7.39 - 7.49
aeration: at least 80% of the air saturation value
- Type of food: Tetra Menü Spezialfutter
- Health during acclimation: fish were in good health - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 2.7 mmol CaCO3/L
- Test temperature:
- 20-21 °C
- pH:
- 7.73 (control)
7.69 (saturated test solution) - Dissolved oxygen:
- At least 80% of the air saturation value
- Nominal and measured concentrations:
- nominal loading rate: 150 mg/L
TOC-values at test start:
test solution (WAF): 1.41 mg/L
control: 1.20 mg/L - Details on test conditions:
- TEST SYSTEM
- Volume: 10 L
- Aeration: permanent during the test duration
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1 – 5.6 g/L (corpulence factor K = 0.8-1.1 g/cm³, length = 5-8 cm)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
- Total organic carbon: 1.2 mg/L
- Acid capacity, KS(4.3): 0.7-0.9 mmol/L
- Inorganic salts: CaCl2: 2.2 mmol/L, MgSO4: 0.5 mmol/L, NaHCO3: 0.64 mmol/L
- Ca/mg ratio: 4:1
- Culture medium different from test medium: yes
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light: 8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality recorded after 48 h - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 150 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: results are expressed in terms of loading rates
- Duration:
- 48 h
- Dose descriptor:
- LL100
- Effect conc.:
- > 150 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: results are expressed in terms of loading rates
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 150 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: results are expressed in terms of loading rates
- Details on results:
- In a saturated solution of the test material in dilution water no mortality of fish after 48 hours was observed. Sub-lethal effects were not reported.
- Validity criteria fulfilled:
- yes
Referenceopen allclose all
Description of key information
96-h LC50 (based on loading rates) >100 mg/L for Oncorhynchus mykiss (OECD TG 203, EU C.1)
48-h LC50 (based on loading rates) >150 mg/L for Leuciscus idus (DIN guideline 38412, part 15)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
The results indicate that the test material is not toxic to rainbow trout up to the limits of its water solubility and a NOEC and thus a PNEC cannot be determined.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.