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EC number: 201-861-7 | CAS number: 88-85-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Dermal absorption
Administrative data
- Endpoint:
- dermal absorption
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- Not reported
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in accordance with generally accepted scientific principles, possibly with incomplete reporting or methodological deficiencies, which do not affect the quality of the relevant results.
Data source
Reference
- Reference Type:
- publication
- Title:
- Age-Related Percutaneous Penetration of 2-sec-Butyl-4,6-dinitrophenol (Dinoseb) in Rats
- Author:
- Hall, L.L. et al.
- Year:
- 1 992
- Bibliographic source:
- FUNDAMENTAL AND APPLIED TOXICOLOGY 19, 258-267
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The effect of age and dosage on the percutaneous absorption and disposition of dinoseb was investigated in female rats. The results were compared with the aid of a preliminary physiological compartmental model. In addition, the dermal absorption determined using two in vitro methods was compared to the in vivo results.
- GLP compliance:
- no
Test material
- Reference substance name:
- Dinoseb
- EC Number:
- 201-861-7
- EC Name:
- Dinoseb
- Cas Number:
- 88-85-7
- Molecular formula:
- C10H12N2O5
- IUPAC Name:
- dinoseb
- Details on test material:
- [14C]dinoseb (uniformly ring labelled, specific activity 4.29 mCi/mmol) was purchased from Pathfinder Labs Inc. Radiochemical purity was greater than 98% as determined by HPLC. Unlabelled dinoseb was obtained from the US EPA Repository for Toxic and Hazardous Material.
Constituent 1
- Radiolabelling:
- yes
Test animals
- Species:
- rat
- Strain:
- Fischer 344
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- Guaranteed time-pregnant Fischer 344 rats were purchased from the Charles River Breeding Farm (Kingston, NY). Male pups were culled from the litters because of the dorsal skin thickening that occurs at sexual maturity. The female pups were randomized and replaced with the dams (eight pups/ dam). At weaning, the females were again randomized and divided into two groups, using rank ordered weights. The first group was used for the studies in young animals and the second group was used for the adult studies. Thirty-three-day-old (DO) animals were used for the young group because the restraints used to protect the application site did not retard growth significantly as they did in younger animals and vaginal opening, a hormone-dependent event, starts at approximately 40 days of age. This provided a 7- day period to perform the studies before puberty began. Eighty-two-DO adult female rats were chosen for logistical reasons. The animals were maintained according to the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Food (Purina Rat Chow, No. 5001, St. Louis, MO) and water were available ad libitum. The animals were maintained on a 12-hr light/dark cycle at constant temperature and humidity. Twenty-four hr prior to in vivo and in vitro experiments, animals were lightly anesthetized with ethyl ether and hair on the backs of the animals was removed with electric clippers using a size 40 blade. The clipped area was then washed with acetone to remove dirt and sebaceous gland secretions.
Administration / exposure
- Type of coverage:
- other: protected by perforated plastic beaker/bubble
- Vehicle:
- acetone
- Duration of exposure:
- The dose-effect animals were terminated at 72 hr and the serially terminated animals at 1, 6, 24, 48, 72, and 120 hr.
- Doses:
- 250(60), 536 (129), and 2680 nmol/cm2 (644 µg/cm2) in the young rat and 210 (52), 536 (129), and 2680 nmol/cm2 (644 µg/cm2). The serial termination studies were performed at 285 nmol/cm2(68 µg/cm2)
- No. of animals per group:
- 3 females per dose and age group
- Control animals:
- no
Results and discussion
- Total recovery:
- Mean recoveries ranged from 87.5 to 105.9% in young and 87.9 to 102% in adult rats.
Any other information on results incl. tables
Mean recoveries ranged from 87.5 to 105.9% in young and 87.9 to 102% in adult rats. Dermal absorption of dinoseb appeared to be biphasic. After 6 hrs, the skin penetration was about 44% in both young and adults, while after 120 hr 75.9% was absorbed in young and 92.5% in adults. The maximum body burden of dinoseb-derived radioactivity observed in young was 41% and occurred at 6 hr, but by 120 hr the body burden had dropped to 5.8%. In adults a maximum observed body burden of 52% occurred at 24 hr and subsequently the body burden declined to 6.8% at 120 hr
Time (hr) |
Dose level
|
Dose level
|
%Absorbed
|
%Absorbed
|
|
|
|
Young |
Adults |
1 |
285 nmol/cm2 |
68µg/cm2 |
11.8 ± 2.2 |
21.1 ± 6.6 |
6 |
285 nmol/cm2 |
68µg/cm2 |
43.0 ± 6.0 |
44.2 ± 10.1 |
24 |
285 nmol/cm2 |
68µg/cm2 |
67.6 ± 3.4 |
86.6 ± 0.8 |
48 |
285 nmol/cm2 |
68µg/cm2 |
61.1 ± 10.5 |
88.1 ± 0.4 |
72 |
285 nmol/cm2 |
68µg/cm2 |
55.6 ± 1.5 |
82.7 ± 1.4 |
120 |
285 nmol/cm2 |
68µg/cm2 |
75.9 ± 1.7 |
92.5 ± 0.8 |
Dose (nmol/cm2)
|
Dose (µg/cm2)
|
Time (hr) |
%Absorbed |
Young |
|
|
|
250 |
60 |
72 |
77.7 ± 5.9 |
535 |
129 |
72 |
81.5 ± 2.8 |
2680 |
2680 |
72 |
82.9 ± 1.0 |
Adult |
|
|
|
210 |
52 |
72 |
86.4± 1.14 |
535 |
129 |
72 |
90.5 ± 1.11 |
2680 |
2680 |
72 |
93.2 ± 0.6 |
The primary route of excretion of dinoseb-derived radioactivity was urinary. By 120 hrs the young excreted about 4.6 and the adult 4.3 times as much in urine as in faeces. Urinary excretion was 57.7 and 69.5% of the recovered dose in young and adults, respectively.
HPLC analysis of 24-h urine samples collected from adults treated with dinoseb showed extensive metabolism. The metabolite profile of radioactivity excreted in urine was similar for the i.v. and dermal treated animals. Detection of parent in the urine samples from the i.v.- and dermal-treated animals was minimal.
Applicant's summary and conclusion
- Conclusions:
- In vivo dermal absorption in both young and adults appeared biphasic with 55.6 and 82.7% of the recovered dose, respectively, penetrating in 72 hr. The primary route of excretion was urinary and by 120 hrs accounted to 57.7 and 69.5%, respectively, in young and adult rats. HPLC analysis of 24-h urine samples showed extensive metabolism, with a similar metabolite profile for the i.v. and dermal treated animals. In vitro measurements of skin absorption at 72 hr with static cells showed higher values in young and lower values in the adult compared to in vivo dermal absorption values. In vitro flow-through measurements at 72 hr gave lower dermal absorption values for both young and adult rats, compared to in vivo values.
- Executive summary:
In a series of studies, the effects of age, dosage, and method of determination on skin absorption, distribution, retention and excretion of dinoseb were examined in female rats.
[14C]dinoseb was applied to previously clipped back skin of 33- and 82-day-old female Fischer 344 rats at a dosage range of 210-2680 nmol/cm2. Radioactivity in the treated skin, tissues, urine, and faeces was determined at 1, 6, 24, 48, 72, and 120 hr following dermal application. In vitro dermal absorption of [14C]dinoseb was also measured in rats of the same age by static and flow-through methods. The metabolic profile in 24-h sample of urine was determined following dermal application and i.v. administration of an equivalent dose.
Dermal absorption was shown to be dependent on age, with skin of adults being more permeable to dinoseb than that of young, and independent of dose. In vivo dermal absorption in both young and adults appeared biphasic with 55.6 and 82.7% of the recovered dose, respectively, penetrating in 72 hr.
The primary route of excretion was urinary, with 57.7 and 69.5% of dose recovered in urine by 120 hrs. HPLC analysis of 24-h urine samples showed extensive metabolism, with similar metabolite profile for i.v. and dermal-treated animals. Detection of parent in the urine samples from the i.v. and dermal-treated animals was minimal.
In vitro measurements of skin absorption at 72 hr with static cells showed higher values in young and lower values in the adult compared to in vivo dermal absorption values. In vitro flow-through measurements at 72 hr gave lower dermal absorption values for both young and adult rats, compared to in vivo values.
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