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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1991-02-19
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study no GLP, acceptable defiencies in reporting

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report date:
1991

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
5,8,11,14,17-pentaoxahenicosane; 5,8,11,14-tetraoxaoctadecane
EC Number:
940-417-3
Molecular formula:
C14H30O4 C16H34O5
IUPAC Name:
5,8,11,14,17-pentaoxahenicosane; 5,8,11,14-tetraoxaoctadecane
Details on test material:
- Name of test material (as cited in study report): Polyglycoldibutylether
- Physical state: Liquid, colourless
- Stability under test conditions: Stable
- Storage condition of test material: Room temperature, protected from light, in original container

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item was weighed out directly in an Erlenmeyer flask, filled up with 200 mL demieralised water.
- Eluate: Test medium:
250 mL inoculum
16 mL synthetic waste water
234 mL autoclaved tap water
- Loading 1000-10000 mg/L (test item)
- Controls: Test medium without test or reference item

Test organisms

Test organisms (species):
activated sludge
Details on inoculum:
- Inoculum from industrial sewage treatment plant 84508 Burgkirchen-Gendorf, Germany
- Pretreatment: Washed twice with autoclaved tap water and adjusted to a dry weight concentration of 3 g/L +/- 10 %. The sludge was used within 24 h after stirring.
- Dry sludge concentration: 3.12 g/L corresponding to 1.56 g/L suspended solids in the test vessels

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

Hardness:
Not determined
Test temperature:
21.1 °C
pH:
7.32 (activated sludge)
7.56 (synthetic waste water)
Salinity:
None, freshwater
Details on test conditions:
- no details given but according Guideline OECD 209
















Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
3 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: n.a.
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: n.a.
Details on results:
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
Results with reference substance (positive control):
- no reference substance used
Reported statistics and error estimates:
- graphical evaluation of data

Any other information on results incl. tables

- graphical data

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The NOEC of the test item is 1000 mg/L and the EC50 3500 mg/L to activated sludge of the industrial sewage treatment plant 84508 Burgkirchen-Gendorf, Germany