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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study in compliance with GLP-criteria. Limitations in reporting but considered adequate for assessment.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report date:
2005

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Ethylenediamine p-toluenesulphonate
EC Number:
230-728-6
EC Name:
Ethylenediamine p-toluenesulphonate
Cas Number:
7294-10-2
IUPAC Name:
ethane-1,2-diamine 4-methylbenzenesulfonate
Details on test material:
- Name of test substance: Ethylendiamin-Monotosylat

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
Activated sludge from laboratory wastewater plants treating municipal sewage. Concentration of dry substance: 30 mg/L .
Duration of test (contact time):
21 d
Initial test substance concentrationopen allclose all
Initial conc.:
44 mg/L
Based on:
test mat.
Initial conc.:
20 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
Test temperature: 22 ± 2 °C
Reference substance
Reference substance:
other: a reference substance was used but not specified in the report

Results and discussion

% Degradation
Value:
90 - 100
Sampling time:
5 d
Details on results:
- Biodegradation degree of the reference substance after 14 days: 90-100% DOC
- Biodegradation degree in the inhibition control after 14 days: 90-100% DOC
- Elimination degree in the abiotic control after 28 days: <10 % DOC
- 10-day window criterion met: Yes

Any other information on results incl. tables

DOC decrease (%)

Day

RS

IH

PC

AC

TS1

TS2

TSmean

0

0

0

0

-

0

0

0

1

-2

0

-2

1

-2

-1

3

95

48

-1

12

10

11

5

94

97

-2

94

103

99

7

96

95

1

92

98

95

10

93

93

-2

95

97

96

14

93

95

-6

95

98

97

17

95

97

-1

95

98

97

20

98

98

1

97

99

98

 21  98  98  2    97  99  98

RS = Reference substance

IH = Inhibition control

PC = Abiotic control

AC = Adsoption control

TS = Test substance

Applicant's summary and conclusion