Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 228-567-1 | CAS number: 6297-03-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997/06/18 - 1997/07/18
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 406 (Skin Sensitisation)
- Version / remarks:
- 17th July 1992
- Deviations:
- no
- GLP compliance:
- yes
- Type of study:
- Buehler test
Test material
- Reference substance name:
- Dioctadecyl ether
- EC Number:
- 228-567-1
- EC Name:
- Dioctadecyl ether
- Cas Number:
- 6297-03-6
- Molecular formula:
- C36H74O
- IUPAC Name:
- 1-(octadecyloxy)octadecane
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- Hartley
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: 76410 Saint-Aubin-Les-Elbeuf, France
- Weight at study initiation: 330g-387g
- Housing: individual polypropylene cages
- Diet: Complete pelleted diet UAR 106 (91360 -Epinay sur Orge, France)
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
in accordance with the requirements of the 86/609/EEC guideline
Study design: in vivo (non-LLNA)
Inductionopen allclose all
- Route:
- epicutaneous, occlusive
- Vehicle:
- other: Mineral oil
- Concentration / amount:
- 50% and 20% of the testsubstance diluted with mineral oil for challenge
Challengeopen allclose all
- Route:
- epicutaneous, occlusive
- Vehicle:
- other: Mineral oil
- Concentration / amount:
- 50% and 20% of the testsubstance diluted with mineral oil for challenge
- No. of animals per dose:
- treated group: 20 animals
negative control group: 10 animals - Details on study design:
- Pilot study
In order to find the maximum non-irritant concentration for the skin a pre-test was carried out. 4 guinea-pigs were closely-clipped at the dorsal level over a surface area of approximately 60 cm2. They received on both sides of the spinal column, for 6 hours under an occlusive patch, 0.5 mL of the test substance, diluted at 50 %, 20 %, 10 % and 5 % with mineral oil, and the vehicle only. The cutaneous irritation reactions were assessed 24 and 48 hours after patches removal. The concentration of test substance retained for the challenge exposure was the highest non-irritating dose.
Determination of the sensitizing potential
For determination of the senstizing potential 20 guinea pigs (group 2) were treated and compared with 10 guinea pigs (group 1) which received the vehicle only (mineral oil).
Induction
Day 1 - treated group (group 2)
Left flank of each guinea-pig was closely-clipped. One hour later, a square cotton pad of 4 cm2 fully loaded (0.5 mL) with test material diluted at 50 % with mineral oil, was applied to the test area and held in contact for 6 hours with the skin by an occlusive patch.
Day 1 - control group (group 1)
Left flank of each animal was closely-clipped. One hour later, the vehicle only (mineral oil), was applied in a similar manner to that used for the treated group. The test patch system was held in contact with the skin by an occlusive patch for 6 hours.
Days 8 and 15 - treated group and control group
One hour before topical applications, left flank of all the animals was closely-clipped. The same application as on day 1 was carried out on the same test area of the same flank on day 8, and again on day 15.
Challenge
Day 29 - treated group and control group
Following a rest period of 14 days, the untreated flank (right flank) of all treated and control animals was closely-clipped. One hour after, we applied under an occlusive patch, to the posterior untreated flank of each of the guinea-pigs, two square cotton pads of 4 cm2 moistened with 0.5 mL of the test material diluted at 50 % (maximum non-irritant concentration) and 20 % with mineral oil and a third cotton pad moistened with vehicle only.
The occlusive patches were held in contact with the skin for 6 hours, then removed.
Day 30 and 31 - treated group and control group
Approximately 24 hours after removing patches, the skin reactions were observed and recorded
Approximately 24 hours later, a second observation (48 hours after patch removal) was made and recorded once again.
All skin reactions and unusual findings observed during the induction and challenge procedures were recorded.
Animals were weighted at the start and at the conclusion of the test. - Positive control substance(s):
- no
Results and discussion
In vivo (non-LLNA)
Resultsopen allclose all
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 20%, 50% of testsubstance diluted with mineral oil
- No. with + reactions:
- 0
- Total no. in group:
- 20
- Clinical observations:
- non
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 24.0. Group: test group. Dose level: 20%, 50% of testsubstance diluted with mineral oil . No with. + reactions: 0.0. Total no. in groups: 20.0. Clinical observations: non.
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 20%, 50% of the test substance diluted with mineral oil
- No. with + reactions:
- 0
- Total no. in group:
- 20
- Clinical observations:
- non
- Remarks on result:
- other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: test group. Dose level: 20%, 50% of the test substance diluted with mineral oil. No with. + reactions: 0.0. Total no. in groups: 20.0. Clinical observations: non.
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- negative control
- Dose level:
- 20%, 50% of the test substance diluted with mineral oil
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Clinical observations:
- non
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 24.0. Group: negative control. Dose level: 20%, 50% of the test substance diluted with mineral oil. No with. + reactions: 0.0. Total no. in groups: 10.0. Clinical observations: non.
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- negative control
- Dose level:
- 20%, 50% of the test substance diluted with mineral oil
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Clinical observations:
- non
- Remarks on result:
- other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: negative control. Dose level: 20%, 50% of the test substance diluted with mineral oil. No with. + reactions: 0.0. Total no. in groups: 10.0. Clinical observations: non.
Any other information on results incl. tables
One animal of the treated group died on day 4. This mortality was not attributable to the treatment but to a lung pathology. The general state of health of all other animals remained satisfactory throughout the test. The weight growth of all the animals was satisfactory.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)