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EC number: 205-359-9 | CAS number: 139-40-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 1977
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Contains sufficient detail to suggest GLP-like characteristics even though no statement of certification is reported (reasonably thorough description of authors, dates, design, results, and interpretation).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 977
- Report date:
- 1977
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: The Committee on Methods for Toxicity Tests with Aquatic Organisms. 1975. Methods for Acute Toxicity Tests with Fish, Macro - invertebrates, and Amphibians. EPA - 660/3/75009 p. 61.
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- other: American Public Health Association. 1971. Standard Methods for the Examination of Water and Wastewater. 13th Ed. New York. 874p.
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- other: Finney, D. J. 1971. Statistical Method in Biological Assay, 2nd Ed. Griffin. London. p. 668.
- Deviations:
- not specified
- Principles of method if other than guideline:
- Daphnids are found in nearly all types of freshwater habitats. They are an important part of the aquatic food web and serve as food for many species of fish. Because of their wide geographic distribution, importance as food web organisms and temperature requirements, D. magna has been recommended by The Committee on Methods for Toxicity with Aquatic Organisms (1975) as a bioassay organism.
- GLP compliance:
- no
Test material
- Reference substance name:
- Propazine
- EC Number:
- 205-359-9
- EC Name:
- Propazine
- Cas Number:
- 139-40-2
- Molecular formula:
- C9H16ClN5
- IUPAC Name:
- 6-chloro-N2,N4-bis(propan-2-yl)-1,3,5-triazine-2,4-diamine
- Details on test material:
- Material: propazine FL-761357
Appaerance: white powder
the test material is soluble in acetone
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not applicable
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- The bioassay was conducted using five concentrations of Propazine, a control, and solvent control. The concentration of acetone in the solvent control equalled the amount in the highest toxicant concentration. Four replicates were run. A stock solution (100 mg/ml) was prepared by weight to a precision of 0.1 mg and diluted to volume in volumetric glassware with reagent grade acetone. The test was conducted in 250 ml glass beakers containing 200 ml of water. The test was started by introducing the toxicant into a 1 liter beaker containing dilution water, thoroughly mixing the toxicant and dilution water and then decanting 200 ml into each of four 250 ml beakers. Five organisms were introduced into each of the test beakers.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna were obtained from a laboratory stock culture. The original stock was obtained from the National Water Quality Laboratory in Duluth, Minnesota. Stock cultures were maintained at 17°C in three liter glass battery jars. Twenty hours prior to starting a bioassay, approximately 15 adults with full brood chambers were isolated into soft lake water. The following morning the newly released instars (less than 20 hours old)
were carefully removed with an eye dropper and distributed to the test beakers.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- no post exposure observation period.
Test conditions
- Hardness:
- Hardness: 48 mg/L as CaCO3
Alkalinity: 26 mg/L as CaCO3 - Test temperature:
- Test temperature was maintained at 17°C ± 1 °C by use of a water bath.
- pH:
- the pH of the test solution has been measured initially and at 48 hours for the control, solvent control, high, medium and low toxicant concentrations, see the table in the box "any other information on material and methods incl. tables"
- Dissolved oxygen:
- The dissolved oxygen of the test solution has been measured initially and at 48 hours for the control, solvent control, high, medium and low toxicant concentrations, see the table in the box "any other information on material and methods incl. tables"
- Salinity:
- Any info about salinity have been reported. It have been reported the hardness, the pH and the conductivity of the dilution water ( see the appropriate boxes).
- Nominal and measured concentrations:
- The propazine nominal concentrations were: 5.6, 10.0, 18.0, 32.0, 56.0 mg/L. The nominal concentrations of the test substance have not neen measured.
- Details on test conditions:
- Dilution water for the daphnid test was obtained from a snail lake located in Westchester County, New York. The water was filtered through a Millipore filter fitted with a Whatman GF/C filter membrane prior to use. First instar daphaids readily survived without feeding for 48 hours so the water was judged to be of acceptable quality for the bioassay. Prior to use the water was intensively aerated. Lake water was characterized as having a pH of 7.43, total hardness of 48 mg/i as CaCO3, total alkalinity of 26 mg/l as CaCO3, and a specific conductivity of 115 pmhos/cm. Hardness and alkalinity were determined according to standard analytical procedures (American Public Health Association, 1971), pH with an ORION ph meter, conductivity with a YSI conductivity bridge and dissolved oxygen with a YSI oxygen meter.
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 45.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% confidencial Interval was 37.7 - 56 mg/L.
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 17.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% Confidencial interval was 13.4 - 23.6 mg/l
- Duration:
- 48 h
- Dose descriptor:
- other: No effect level
- Effect conc.:
- < 5.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- The 48 hour LC50 with 95% confidence limits for Propazine to D. magna is 17.7 (13.4 - 23.6) mg/i. The 48 hour no effect level was observed to be less than 5.6 mg/l. It should be noted that LC5O values may vary with different species, temperatures and water qualities.
- Results with reference substance (positive control):
- not applicable
- Reported statistics and error estimates:
- The concentration of toxicant lethal to 50% of the population (LC50) and the 95% confidence limits were determined by the Spearman-Karber Estimator (Finney, 1971).
Any other information on results incl. tables
Percent mortality:
Propazine nominal conc. Mg/L |
control |
Solvent control |
5.6 |
10.0 |
18.0 |
32.0 |
56.0 |
24 hours |
0% |
0% |
5% |
5% |
15% |
10% |
50% |
48 hours |
0% |
5% |
30% |
45% |
50% |
50% |
75% |
LC50 values:
24 hours 48 hours
LC50 mg/L
|
45.9 |
17.7 |
|
95% Confidence Interval
|
Low |
37.7 |
13.4 |
High |
56.0 |
23.6 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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