Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 208-488-9 | CAS number: 530-62-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Micronucleus assay
- Type of information:
- other: hydrolysis product
- Adequacy of study:
- other information
- Study period:
- 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- review article or handbook
- Title:
- Unnamed
- Year:
- 2 003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- Imidazole
- EC Number:
- 206-019-2
- EC Name:
- Imidazole
- Cas Number:
- 288-32-4
- Molecular formula:
- C3H4N2
- IUPAC Name:
- 1H-imidazole
- Details on test material:
- 99,5%
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- olive oil
- Duration of treatment / exposure:
- 24h/48h
- Frequency of treatment:
- single dose
- No. of animals per sex per dose:
- 5
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
Any other information on results incl. tables
Range finding study:
During pretests deaths were observed down to a dose of 2250 mg/kg bw whereas 2000 mg/kg bw was survived by all animals but led to signs of toxicity including irregular
respiration, piloerection, squatting posture in some cases, closed eyelids, and saltatory convulsions.
Main Study:
Analytical examination of olive oil samples revealed concentrations of 52.9, 100.4, and 198.0 g/l, i.e. 99-106% of the theoretical values. Substance stability was verified.
Clinical signs:
none after vehicle or after positive control substances. Signs following TS at 500 mg/kg bw: irregular respiration and piloerection 15-30 min after dosing; at 1000 mg/kg bw additionally closed eyelids in some cases within 15-30 min; at 2000 mg/kg bw: additionally squatting posture in some animals; one high dose animals was found dead within 1 h after dosing. No signs were noted in positive control animals.
Mean micronuclei counts at 24 hr after dosing, expressed as 0/oo in polychromatic (=pe) and total number of normochromatic (=ne) erythrocytes containing micronuclei:
Solvent control: pe=2.6 ne= 0.3
cyclophosphamide: pe=8.6 ne= 1.6
vincristine: pe= 15.4 ne= 3.0
TS, 500 mg/kg bw: pe=2.2 ne= 0.4
TS, 1000 mg/kg bw: pe=1.7 ne= 0.4
TS, 2000 mg/kg bw: pe=2.3 ne= 0.3
Other time intervals
TS, 2000 mg/kg bw, 16 h; pe= 1.9 ne= 1.1
TS, 2000 mg/kg bw, 48 h; pe= 1.4 ne= 0.9
Compared to control animals imidazole did not change the incidence of micronuclei in polychromatic or normochromatic erythrocytes at any time or dose. The ratio of polycromatic to normochromatic erythrocytes was unchanged.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
No significant or biologically relevant differences were
noted between treated and solvent control group animals.
Thus, imidazole showed no clastogenic (DNA-damaging)
property, nor did it impair chromosome distribution due to a spindle poison effect.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)