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EC number: 232-260-8 | CAS number: 7803-51-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well described study with a consistant and relevant material & methods.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- Acute and subacute inhalation toxicty of highly purified phosphine (PH3) in male ICR mice
- Author:
- Omae K., Ishizuka C. and Nakashima H
- Year:
- 1 996
- Bibliographic source:
- J Occup Health ; 38 : 36-42
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Phosphine
- EC Number:
- 232-260-8
- EC Name:
- Phosphine
- Cas Number:
- 7803-51-2
- Molecular formula:
- H3P
- IUPAC Name:
- phosphane
- Details on test material:
- The 99.995% pure PH3 used in semiconductor manufacturing (Nippon Sanso, Co).
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- ICR
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- Four week old male ICR mice were purchased from Charles River. After one week of acclimatation, they were used in the inhalation experiments. Five mice each were housed in plastic cages in a filtrated air-ventilated rack for small animal breeding. The cages were maintained at approximately 22°C, 60% relative humidity, and a 12 hour light, 12 hour dark cycle and fed pelleted rodent chow and water ad libitum throughout both the acclimation and observation period.
Administration / exposure
- Route of administration:
- inhalation: gas
- Type of inhalation exposure:
- whole body
- Vehicle:
- air
- Details on inhalation exposure:
- The source gas was diluted with purified nitrogen. It was supplied at a constant rate, mixed with filtered room air, and introduced into the whole-body 550-L exposure chamber.
- Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- Phosphine concentrations were measured via gas chromatography every 12 min during exposure. The oxygen concentration in the chamber was measured simultaneously with a digital oximeter.
- Duration of exposure:
- >= 1 - <= 8 h
- Remarks on duration:
- All exposures were followed by a 2-week observation period
- Concentrations:
- Experiment to determine LC50
First experiment: 17.2 ppm (+/- 1.3) ; 25.1 ppm (+/- 0.9) ; 31.7 ppm (+/- 1.4) ; 41.6 ppm (+/- 1.4) ; 59.2 ppm (+/- 2.0) for 1 hour
Second experiment: 22.5 ppm (+/- 3.8) ; 26.5 ppm (+/- 2.4) ; 33.4 ppm (+/- 2.6) ; 45.5 ppm (+/- 4.0) ; 66.9 (+/- 5.0) for 4 hours
Acute exposure experiment
Third experiment: 24.5 ppm (+/- 2.0) for 1 hour ; 23.9 ppm (+/- 1.6) for 2 hours ; 24.5 ppm (+/- 1.0) for 4 hours ; 24.9 ppm(+/- 1.2) for 8 hours - No. of animals per sex per dose:
- 10 mice per group
- Control animals:
- yes
- Details on study design:
- The mice were weighted, their behavior and external appearance were observed.
The observation period for the experiment to determine the LC50 was set at two weeks.
The blood was collected, three days after the exposure, from the axillary vessels under diethyl ether anesthesia and the liver, kidneys, spleen, lung, pancreas, brain and testes were promptly removed and then weighed.
The sciatic nerve, the skull for nasal cavity examination, and the femoral bone for bone marrow analysis were also removed.
Serum biochemical and hematological items examined were enzymic activites of AST, ALT, ALP, Ch-E, concentrations of Blood urea nitrogen and total counts of red blodd cells and white blood cells and the differential count. - Statistics:
- Student's t-test of Welch's t-test was used for statistical testing of the difference in the mean of the effect variable for exposed and control mice.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- > 59.2 ppm
- Based on:
- test mat.
- Exp. duration:
- 1 h
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- > 26.5 - < 33.4 ppm
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- Experiment to determine LC50
Table in the "other information" part shows the number of deaths and exposure concentrations.
Acute exposure experiment
All of the animals in the one, two and four hour exposure group survived, and all of the animals in the eight hour exposure group died (four immediately before the completion of exposure and six by day three). - Clinical signs:
- other: In all of the 1-h exposed animals, the mice exhibited face-washing movements and were very active in the initial exposure period. No adverse signs were noted during the 3-day observation period after exposure. In the 4-h exposed mice, the initial observat
- Body weight:
- Body weight significantly decreased in the two and four-hour exposure groups when compared with the control group and the survival animals in the eight-hour exposure group also showed obvious weight loss.
- Gross pathology:
- Organ weights revealed significant differences from the control group in kidney weight in the one-hour exposure group, the heart in the two-hour exposure group, and the kidney, testes and heart in the four-hour exposure group but there was no clear trand in relation to the duration of exposure.
- Other findings:
- Experiment to determine LC50
In all groups, the mice appeared to do face washing mouvments and were extremely active in the initial period after the start of exposure.
In the 33.4 ppm and above exposure groups: the reactions of the mice to the noise made by tapping the wall of the exposure chamber became slower, and a supine posture was observed.
Acute exposure experiment
In the two, four and eight hour exposure groups, in the initial period after the stard of exposure, the animals were extremely active.
In the eight hour exposure group, spontaneous motor activity began to decrease approximately one hour after the start of exposure and at approximately three hours, the animal's reactions to the noise made by tapping the wall of the exposure chamber became slower and supine position was observed.
Any other information on results incl. tables
Experiment to determine LC50
The table 1 shows the number of deaths and exposure concentrations.
Exposure concentration (ppm) | Mortality | |
One hour exposure | 17.2 (+/ - 1.3) | 0/10 |
25.1 (+/- 0.9) | 0/10 | |
31.7 (+/- 1.4) | 0/10 | |
41.6 (+/- 1.4) | 0/10 | |
59.2 (+/- 2.0) | 0/10 | |
Four hours exposure | 22.5 (+/- 3.8) | 0/10 |
26.5 (+/- 2.4) | 0/10 | |
33.4 (+/- 2.6) | 10/10 | |
45.5 (+/- 4.0) | 10/10 | |
66.9 (+/- 5.0) | 10/10 |
Applicant's summary and conclusion
- Interpretation of results:
- very toxic
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The acute inhalation toxicity of PH3 in male ICR mice was investigated. LC50 for one hour exposure was greater then 59.2 ppm and that for four-hour expsure was between 26.5 ppm ande 33.4 ppm.
On the basis of LC50 for four hours, the phosphine is classified as T+, R26 and Acute Tox. Categorie I, H330. - Executive summary:
The acute inhalation toxicity of PH3 in male ICR mice was investigated. LC50 for one hour exposure was greater then 59.2 ppm and that for four-hour expsure was between 26.5 ppm ande 33.4 ppm. Experiment involving acute exposure to 25 ppm PH3 for one, two, four of eight hours were conducted. All mice subjected to acute eight-hour exposure died but a histopathological examination failed to reveal the actual causes of death. In the nasal cavity, exposure-time related inflammatory changes were observed in the acute two-, four- and eight hour exposure groups. Other histopathological, hematological, and serum biochemical examination dit not reveal PH3 related changes.
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