Benzenethiol

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2001

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Benzenethiol

EC Number:

203-635-3

EC Name:

Benzenethiol

Cas Number:

108-98-5

Molecular formula:

C6H6S

IUPAC Name:

benzenethiol

Details on test material:

- Name of test material : benzenthiol
- Molecular formula : C6H6S
- Molecular weight : 110.18
- Substance type: liquid (a clear, colorless)
- Analytical purity: >98.0%
The determination of purity and molecular fomula were by IR , NMR and HPLC.
- Lot/batch No.:E99776L
- Storage condition of test material: at room temperature under dark

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: -14.8℃
- Boiling point: 168℃
- Vapour pressure:0.13kPa (18℃)
- Water solubility (under test conditions): 836mg/L
- log Pow:2.52
- pKa: 6.62

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Sampling method:
volume : 5 mL
sampling point : 0,1,7,8,14,and 15 day
The concentration of the test substance in the samples was determined by GC method.

Test solutions

Vehicle:

yes

Details on test solutions:

- Method:
(1)A stock solution of 100mg/L of test subsances was prepared at the beginning of the test. A stock solution diluted with M4 medium to 1mg/L .
(2)The positive control was dilution water.
(3)Ten test vessels was prepared each concentrations. Each test vessels were added 80mL of the test solutions.
(4)All test vessels were checked that those appearance.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name:Daphnia magna
- Source:National Institute for Environmental studies
Feeding during test
- Food type:Chlorella vulugaris
- Amount:0.1~0.2mgC/day/one Daphnia magna

ACCLIMATION
- Acclimation period: 28days
- Breeding dencity : 20~50 animals/L test water
- Acclimation conditions :same as test
- Type and amount of food: Chlorella vulugaris
- Ligthting : interior light ,16-hour light/8-hour dark cycle
- Type and amount of food:0.1mg~0.2mgC/day/one Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

21 d

Post exposure observation period:

no

Test conditions

Hardness:

252mg/L (as CaCO3 ) (M4 medium)

Test temperature:

20±1℃

pH:

pH values was measured during the test. It was around pH7.2-7.6 during the test. It was pH7.3 in M4 medium.

Dissolved oxygen:

oxygen concentration was measured during the test. It was around 7.5-9.0 (mg/L) during the test.

Salinity:

not apparatus

Nominal and measured concentrations:

not apparatus

Details on test conditions:

TEST SYSTEM
- Test vessel: 100-mL glass vessel

TEST MEDIUM
- Stock culture medium: M4 medium

following composition:
----------------------------
H3BO3: 2.860 mg/l
MnCl2.4H2O: 0.361 mg/l
LiCl: 0.306 mg/l
RbCl: 0.071 mg/l
SrCl2.6H20: 0.152 mg/l
NaBr: 0.016 mg/l
Na2MoO4.2H2O: 0.063 mg/l
CuCl2.2H2O: 0.017 mg/l
ZnCl2: 0.013 mg/l
CoCl2.6H20: 0.010 mg/l
KI: 0.00325 mg/l
Na2SeO3: 0.00219 mg/l
NH4VO3: 0.00058 mg/l
Na2EDTA.2H20: 2.5 mg/l
FeSO4.7H2O: 0.996 mg/l
MgSO4.7H2O: 123.3 mg/l
KCl: 5.8 mg/l
NaHCO3: 64.8 mg/l
NaNO3: 0.274 mg/l
KH2PO4: 0.143 mg/l
K2HPO4: 0.184 mg/l
Thiamine hydrochloride: 0.075 mg/l
Cyanocobalamine(V.B12): 0.001 mg/l
D(+)Biotin(V.H): 0.00075 mg/l
CaCl2.2H2O: 293.8 mg/l
Na2SiO3.9H2O: 10.0 mg/l
-------------------------------------

RANGE-FINDING STUDY
- Results used to determine the conditions for the definitive study: 48hr-EiC50 =0.0044mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

>= 3.3 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

< 3.7 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: 95% (<4.6%)

Effect concentrations 3

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

< 2 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other:

Effect concentrations 4

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

3.3 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other:

Details on results:

see attached document.

Reported statistics and error estimates:

Statistical methods: Bartlett's test, ANOVA, Dunnett's test,
EC50, LC50 and NOEC were calculated by probit analysis, using the program Probit.

Any other information on results incl. tables