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EC number: 208-953-6 | CAS number: 548-62-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 1972
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other:
- Principles of method if other than guideline:
- The test material, suspension in liquid water was administered by intragastric infection to young Adult ChR-CD male rats in single doses. Survivors were sacrificed 14 days later.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- [4-[4,4'-bis(dimethylamino)benzhydrylidene]cyclohexa-2,5-dien-1-ylidene]dimethylammonium chloride
- EC Number:
- 208-953-6
- EC Name:
- [4-[4,4'-bis(dimethylamino)benzhydrylidene]cyclohexa-2,5-dien-1-ylidene]dimethylammonium chloride
- Cas Number:
- 548-62-9
- Molecular formula:
- C25H30ClN3
- IUPAC Name:
- 4-{bis[4-(dimethylamino)phenyl]methylidene}-N,N-dimethylcyclohexa-2,5-dien-1-iminium chloride
- Test material form:
- solid
- Details on test material:
- - Name (IUPAC): 4-{bis[4-(dimethylamino)phenyl]methylidene}-N,N-dimethylcyclohexa-2,5-dien-1-iminium chloride
- Name of test material (as cited in study report): Gentian Violet
- Molecular formula: C25H30ClN3
- Molecular weight: 407.986 g/mol
- Smiles notation): CN(C)C1=CC=C(C=C1)C(=C2C=CC(=[N+](C)C)C=C2)C3=CC=C(C=C3)N(C)C.[Cl-]
- InChl : InChI=1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
- Substance type: organic
- Physical state: Solid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: ChR - CD
- Sex:
- male
Administration / exposure
- Route of administration:
- other: Oral : distilled water
- Vehicle:
- other: Intragastric intubation
- Doses:
- 11,000
7,500
5,000
3,400
2,250
1,500
1,000
670
450
200
130
Results and discussion
Effect levels
- Sex:
- male
- Dose descriptor:
- other: Approximate Lethal Dose
- Effect level:
- 670 mg/kg bw
- Based on:
- test mat.
- Mortality:
- Suspension Dose Mortality
% + (Mg/Kg)+
50 11,000 D-1
50 7,500 D-3 ½ hrs
50 5,000 D-4 ½ hrs
50 3,400 D-2 d.
50 2,250 D-2 d.
50 1,500 D-5 d.
25 1,000 D-4 d.
5 670 D-7 d.
5 450 S-14 d.
5 200 S-14 d.
5 130 S-14 d. - Clinical signs:
- other: Lethal Dose-Stained perineal area, eyes half closed, lethargy, Weakness, Ruffed coat, Diarrhea, pallor and irregular respiration; Salivation, anuria and mucoid diarrhea at 2,250 mg/kg and above; belly-to-cage posture at 3,400 mg/kg and above; weight loss.
Applicant's summary and conclusion
- Interpretation of results:
- Toxicity Category IV
- Remarks:
- Migrated information Criteria used for interpretation of results: other: Test Result
- Executive summary:
Du Pont paper Blue R solution is slightly toxic when administered to young adult ChR -CD male rats in single doses; it’s approximate lethal dose(ALD) is 670mg/kg of body weight. since the oral LD50 for acetic acid alone is approximately 3,300 mg/kg for rats, it would appear that the Relatively high toxicity observed with the subject compound cannot be explained by the presence of acetic acid in the mixture.
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