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EC number: 219-135-3 | CAS number: 2370-63-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-12-15 to 1998-01-13
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- 04 April 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- adopted 07 June 1984
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 4.3, 9.4, 20.7, 45.5, 100, 200 mg/L; at beginning and end of the test
- Sample storage conditions before analysis: no data - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Preliminary test: Preparation of a stock solution (106.9 mg of test item in 1.069 L dilution water) by stirring for 23.5 h in darkness
- Definitive test: Preparation of a stock solution (408 mg in 2.044 L dilution water) by stirring in darkness for 58 h. Resulting concentration: 200 mg/L
- Preparations of test concentrations: dilution of the stock solution by the factor of 2 (100 mg/L) and then 2.2 (45.5, 20.7, 9.4, 4.3 mg/L) - Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: Pseudokirchneriella subcapitata CCAP 27814
- Source (laboratory, culture collection): Culture Center of Algae and Protozoa
- Age of inoculum (at test initiation): 4 d pre-culture
- Method of cultivation:
4 d before the test, pre-cultures were prepared by diluting 5 mL of the stock culture in 500 mL dilution water; at the beginning of the test, 50 mL were transferred from the pre-culture and cell numbers were adjusted to 10E04 cells/mL
ACCLIMATION
- Culturing media and conditions (same as test or not): same as test
- Any deformed or abnormal cells observed: no; verified for each culture before use - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 22.75 ± 1.75 °C
- pH:
- initial: 7.68 - 7.79
final: 7.27 - 7.66 - Dissolved oxygen:
- initial: 9.1 - 9.6 mg/L
final: 8.9 - 9.1 mg/L - Nominal and measured concentrations:
- preliminary test: 0.1 to 100% of saturated solution
definitive test:
nominal: 0, 4.3, 9.4, 20.7, 45.5, 100, 200 mg/L
measured, initial: 0, 4, 8.6, 19.1, 42.4, 92.9, 184.2 mg/L
measured, final: 0, 3.6 (2.65*), 7.75, 17.1, 39.8 (35.1*), 84.3, 170.05 (159.7*) mg/L
*concentrations measured using samples from algae assay. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL glass erlenmeyer flasks containing 50 mL test solution and algae inoculum
- Type (delete if not applicable): closed
- Initial cells density: 10E04 cells/mL
- Control end cells density: 2.10E06 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: 16 h light; 8 h dark
- Stirring: 150 rpm
- light intensity: 6000 to 7100 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: fluorescence measurement using a cytofluorimeter after 0, 24, 48, 72 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
Range finding study
- Test concentrations: 0.1 to 100% of saturated solution
- Results used to determine the conditions for the definitive study: result of the preliminary study not given in the study report; results were used to determine the limit concentration used in the definitive test - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 22 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 7.3 - 51 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 184.2 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 4.4 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL: 0.53 - 10 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 37 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL: 18-90 mg/L
- Details on results:
- - Observation of abnormalities: Some algae exposed to the 2 highest concentrations appeared turgid in comparison to the control.
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: 72 h EC50 biomass: 0.36 mg/L, growth rate: 0.9 mg/L (last test: 1997-10-24) - Reported statistics and error estimates:
- Dunnett's test
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study ETMA was found to inhibit the growth of the freshwater green alga Pseudokirchnerella subcapitata after 72 hours.
The NOEC, EC10 and EC50 values based on growth rate were 4.0 mg/L, 22.0 mg/L and >184.2 mg/L, respectively.
The NOEC, EC10 and EC50 values based on biomass were 4.0 mg/L, 4.4 mg/L and 37.0 mg/L, respectively. All effect levels are given based on initial measured concentrations of ETMA. - Executive summary:
In a 72 hour toxicity study, the cultures of Pseudokirchneriella subcapitata CCAP 27814 were exposed to ETMA at nominal concentrations of 0 (control), 4.3, 9.4 20.7, 45.5, 100 and 200 mg/L under static conditions in accordance with OECD Guideline 201, adopted 07 June 1984, and EU test method C.3, 04 April 1992.
Microscopic observation showed that some algae exposed to the 2 highest concentrations appeared turgid in comparison to the control.
The NOEC, EC10 and EC50 values based on growth rate were 4.0 mg/L, 22.0 mg/L and >184.2 mg/L, respectively. The NOEC, EC10 and EC50 values based on biomass were 4.0 mg/L, 4.4 mg/L and 37.0 mg/L, respectively. All effect levels are given based on initial measured concentrations of ETMA.
Results Synopsis
Test Organism: Pseudokirchnerella subcapitata CCAP 27814
Test Type: Static
72 hr ErC50: > 184.2 mg/L
72 hr EyC50: 37 mg/L 95% C.I.: 18-90 mg/L
72 hr ErC10: 22 mg/L 95% C.I.: 7.3 - 51 mg/L
72 hr EyC10: 4.4 mg/L 95% C.I.: 0.53 - 10 mg/L
72 hr NOEC: 4.0 mg/L
Endpoint(s) Effected: growth rate, biomass
Reference
concentration, nominal (mg/L) |
biomass (%) |
growth rate (%) |
0 |
0.00 |
0.00 |
4.3 |
5.49 |
1.01 |
9.4 |
29.71 |
7.63 |
20.7 |
45.47 |
14.38 |
45.5 |
46.18 |
14.41 |
100 |
57.80 |
17.36 |
200 |
88.18 |
43.30 |
Description of key information
72 hr ErC50 > 184.2 mg/L; 72 hr ErC10=22 mg/L (95% C.I.: 7.3 - 51 mg/L); OECD guideline 201/EU method C.3, static; GLP
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 184.2 mg/L
- EC10 or NOEC for freshwater algae:
- 22 mg/L
Additional information
One study in freshwater algae is available for ETMA, which is considered reliable without restriction (Klimisch score 1).
In a 72 hour toxicity study, the cultures of Pseudokirchneriella subcapitata CCAP 27814 were exposed to ETMA at nominal concentrations of 0 (control), 4.3, 9.4 20.7, 45.5, 100 and 200 mg/L under static conditions in accordance with OECD Guideline 201, adopted 07 June 1984, and EU test method C.3, 04 April 1992.
Microscopic observation showed that some algae exposed to the 2 highest concentrations appeared turgid in comparison to the control.
The NOEC, EC10 and EC50 values based on growth rate were 4.0 mg/L, 22.0 mg/L and >184.2 mg/L, respectively. The NOEC, EC10 and EC50 values based on biomass were 4.0 mg/L, 4.4 mg/L and 37.0 mg/L, respectively. All effect levels are given based on initial measured concentrations of ETMA.
According to OECD guideline 201 (adopted July 2011), the EC10 is preferred as relevant dose descriptor.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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