Acetamide, N-[5-[bis(2-methoxyethyl)amino]-2-[2-(2,6-dicyano-4-nitrophenyl)diazenyl]phenyl]-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

03.02.2020 - 31.03.2020

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2020

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

13 April 2004

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

437-930-5

EC Name:

-

Cas Number:

51553-03-8

Molecular formula:

Hill formula: C22H23N7O5 CAS formula: C22H23N7O5

IUPAC Name:

N-{5-[bis(2-methoxyethyl)amino]-2-[2-(2,6-dicyano-4-nitrophenyl)diazen-1-yl]phenyl}acetamide

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The samples were filled into glass vials, stabilised with 50% acetonitrile and stored in the freezer (≤ -18 °C). The sampling and documentation were conducted according to the following specification: After 0 h, 24 h and 48 h exposure, two replicates of 4 ml each were sampled from NC and A. Per replicate 1 ml of each test vessel were pooled to obtain 4 ml in total. The samples were filled into 12 mL-glass screw-cap vials. To each vial, 4 mL acetonitrile was added and samples were stored in the freezer (≤ -18 °C). Of each sampled treatment, one of the samples from 0 h, 24 h and 48 h was sent to the analytical laboratory at the test site biochemA GmbH for chemical analysis using an insulated box with thermal packs. The remaining samples were stored as retain samples in the freezer (≤ -18 °C) until finalisation of the study.

Test solutions

Vehicle:

yes

Details on test solutions:

Test item concentration was below the limit of calibration (LOC = 1 mg/L) in the saturated test item solution as well as in the negative control. Therefore, effect concentrations could not be calculated based on exposure concentrations but are given in relation to the nominal loading rate.

Test organisms

Test organisms (species):

other: Daphnia magna Strauss

Details on test organisms:

The test organism Daphnia magna Straus originates from the clone breeding of the Federal Environment Agency, department IV 2.4, Berlin, and has been cultivated at Hydrotox GmbH since October 2012. The daphnids are cultured at 20  2 °C with 16 h light : 8 h dark and placed into fresh Elendt M4 medium twice a week. They are kept at approx. 10 animals / 200 mL and fed on workdays with Desmodesmus subspicatus algae corresponding to 0.1 mg C × daphnid-1 × day-1. The test organisms used were 1.75 – 21.25 h old at the start of the test.

Study design

Test type:

static

Limit test:

yes

Total exposure duration:

48 h

Remarks on exposure duration:

The swimming capability of the daphnids was assessed after 24 h and 48 h exposure.

Test conditions

Test temperature:

20.2 - 21.3 °C

pH:

7.6 - 7.7

Dissolved oxygen:

7.5 - 8.8 mg/L

Conductivity:

conductivity (695 μS/cm)

Nominal and measured concentrations:

Test item concentration was below the limit of calibration (LOC = 1 mg/L) in the saturated test item
solution as well as in the negative control. Therefore, effect concentrations could not be calculated
based on exposure concentrations but are given in relation to the nominal loading rate.

Details on test conditions:

- The pH was 7.6 – 7.7 in the control as well as in the test item treatment (recommended: 6 – 9).
- The dissolved oxygen concentration was 7.5 – 8.8 mg/L in the control and 7.3 – 8.7 mg/L in the test item treatment (required: ≥ 3 mg/L).
- The temperature during the exposure was 20.2 – 21.3 °C (required: 18 – 22 °C, constant within +- 1 °C).

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

24 h

Dose descriptor:

LOELR

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

other: loading rate

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

24 h

Dose descriptor:

NOELR

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

other: loading rate

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

LOELR

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

other: loading rate

Basis for effect:

mobility

Effect concentrations 4

Key result

Duration:

48 h

Dose descriptor:

NOELR

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

other: loading rate

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes