Trisodium 2,4-diamino-3,5-bis-[4-(2-sulfonatoethoxy)sulfonyl)phenylazo]benzenesulfonate

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Dec. 25, 1998 to Apr. 01, 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: WP2/96
- Expiration date of the lot/batch:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability under test conditions: stable during the exposure period,
- Solubility = Water >/= 100 g/L

Radiolabelling:

not specified

Details on sampling:

The concentrations of the test substance in the test water for both level 1 and 2 were analyzed 16 times ; twice a week intervals (n=1).
The concentrations of peak 1 in the test fish for both levels were analyzed 4 times; in weeks 2, 4, 6 and 8 (n=2).
The control fish were analyzed at the initiation and the termination of exposure (n=2).
The concentrations of the converted products which was pretreated with a protease in the test fish for both levels were analyzed 1 time; in weeks only 8 (n=2). The control fish were analyzed at the termination of exposure (n=2).

Vehicle:

no

Details on preparation of test solutions, spiked fish food or sediment:

Preparation of stock solutions
Level 1: 1200 mg/L stock solution was prepared with ion-exchanged water.
Level 2: 120 mg/L stock solution was prepared with ion-exchanged water.

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Test fish: Carp
Supplier: Sugishima fish farm
Weight average: 22.3 g
Length average: 9.7 cm

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

8 wk

Hardness:

no data

Test temperature:

25 ± 2 °C

pH:

no data

Dissolved oxygen:

no data

TOC:

no data

Salinity:

no data

Details on test conditions:

Observation of test fish: Condition of test fish was observed visually twice a day.
Temperature of test water: Temperature of test water was measured with alcohol thermometer and recorded once a day.
Concentration of dissolved oxygen in test tank: Concentration of dissolved oxygen in test tank was measured with dissolved oxygen probe and recorded twice a week.

Nominal and measured concentrations:

Level 1: 3 mg/L (as the test substance)
Level 2: 0.3 mg/L (as the test substance)

Reference substance (positive control):

not specified

Details on estimation of bioconcentration:

no data

Remarks on result:

other: The bioconcentration factors were <=0.6 in level 1 and <=5.6 in level 2 for peak 1, and <=4.1 in level 1 and <=40 in level 2 for peaks 1, 2 and 3.

Details on kinetic parameters:

no data

Metabolites:

no data

Results with reference substance (positive control):

no data

Details on results:

Test water analysis: The measured concentrations of the test substance in test water were maintained at more than 80 % (peak 1) and more than 135 % (peak 2), It was reported that parts of the test substance were converted in the test water in the biodegradation test (Test No. 13155), It was considered that the measured concentrations of peak 1 were lower and peak 2 were higher than the nominated concentrations, because a part of peak 1 was also converted to peak 2 in this test.

Test fish analysis: It was suggested that the test substance (peak 2 and peak 3) was adsorbed by or reacted with fish body, because it was not recovered from test fish. Therefore, the fish body was pretreated with a protease, and substances which seemed to originate from the test substance were recovered. It is considered that they were substances converted by the pretreatment with protease, named “converted products” hereafter, because they were different from the test substance in the retention time of HPLC. The recovery rate of the converted products was 63 % when it was determined based on a comparison with the test substance.

Reported statistics:

no data

Validity criteria fulfilled:

yes

Conclusions:

The bioconcentration factors were <=0.6 in level 1 and <=5.6 in level 2 for peak 1, and <=4.1 in level 1 and <=40 in level 2 for peaks 1, 2 and 3.

Executive summary:

Bioaccumulation test of FAT45'176/A in carp was performed under GLP compliance according to OECD 305C guideline.

Test fish: Carp (Cyprinus carpio)

Nominal concentrations of test substance:        High exposure level (level 1)                       3 mg/L

                                                                                Low exposure level (level 2)                       0.3mg/L

Duration of exposure: 8 weeks

Exposure method: Continuous flow system

Analytical method: High performance liquid chromatography

The 48-h LC₅₀value was >/=1000 mg/L the bioconcentration factors were<=0.6 in level 1 and <=5.6 in level 2 for peak 1, and <=4.1 in level 1 and <=40 in level 2 for peaks 1, 2 and 3.

Description of key information

The 48-h LC50value of Reactive Orange 136 was >/=1000 mg/Lthe bioconcentration factors were<=0.6 in level 1 and <=5.6 in level 2 for peak 1, and <=4.1 in level 1 and <=40 in level 2 for peaks 1, 2 and 3.

Additional information

Bioaccumulation test of FAT45'176/A in carp was performed under GLP compliance according to OECD 305C guideline.

Test fish: Carp (Cyprinus carpio)

Nominal concentrations of test substance:        High exposure level (level 1)                       3 mg/L

                                                                               Low exposure level (level 2)                       0.3mg/L

Duration of exposure: 8 weeks

Exposure method: Continuous flow system

Analytical method: High performance liquid chromatography

The 48-h LC₅₀value was >/=1000 mg/Lthe bioconcentration factors were<=0.6 in level 1 and <=5.6 in level 2 for peak 1, and <=4.1 in level 1 and <=40 in level 2 for peaks 1, 2 and 3.