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EC number: 468-880-2 | CAS number: 102985-93-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004-08-18 to 2004-11-30
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- adopted 1988
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: For the pre-test a stock solution containing 200 mg test item/L in deionized water was prepared. After 10 minutes in the ultrasonic bath the emulsion didn’t show any unsolved particles. The stock solution then was used to prepare the solutions of the concentrations 100, 10 and 1 mg/L. Because of the low water solubility, the treatment 1000 mg/L was prepared by adding the appropriate volume of the test item directly.
In the main study, the test item was used directly. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Preparation of inoculum for exposure: The nutrient solution was freshly prepared; the inoculum was taken from its source, washed, aerated and the dry matter was determined. The stock solutions of the test item and the reference item were prepared.
- Pretreatment: The sludge was washed with tap water three times, then centrifuged and resuspended in tap water. 200 mL of the sludge were added to each test vessel. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 °C
- pH:
- Pre-Test: 7.7
Main Study: 7.3 - Nominal and measured concentrations:
- Pre-test: 1; 10; 100; 1000 mg/L (nominal)
Main-study: 1000 mg/L (nominal) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers
- Aeration: purified air, using Pasteur pipettes, flow approx. 0.75 L/min
Pre-test:
- No. of vessels per concentration (replicates): one replicate/treatment
- No. of vessels per control (replicates): one replicate before and one after measuring O2-concentrations in the test item replicates
- Biomass loading rate: 0.9086 g/cm³
Main-Study:
- No. of vessels per concentration (replicates): two replicates/treatment
- No. of vessels per control (replicates): two replicates before, two between and two after measuring O2-concentrations in the reference and test item replicates
- Biomass loading rate: 0.9086 g/cm³
TEST MEDIUM / WATER PARAMETERS
- in accordance to the guideline - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenole
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- The reference item showed an EC50 of 8.2 mg/L in the main study, which is within the recommended range of 5 – 30 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 3 h EC50 was determined to be greater than 1000 mg/L and the 3 h NOEC was 1000 mg/L.
- Executive summary:
This study was performed in order to evaluate the effect of the test item on the respiration of activated sludge obtained from domestic sewage treatment plant. The activated sludge respiration test is a rapid screening method to identify substances which may adversely affect aerobic microorganisms. In the pre-test, the test item was tested using four concentrations (ranging from 1000 to 1 mg/L). Duration of the test was three hours. Activated sludge was used as inoculum. It was taken from a domestic sewage treatment plant and washed before usage. The dry matter was determined as 4.0 g suspended solids/L, giving a concentration of 1.6 g suspended solids/L in the test. As the pre-test was performed for range-finding only, no reference item was tested. No inhibition of the respiration of the activated sludge was detected in the treatments. The main study was performed as a limit-test with the nominal concentration of 1000 mg/L. Conduct of the study was the same as in the pre-test. The dry matter of the activated sludge was determined as 4.1 g suspended solids/L, giving a concentration of 1.64 g suspended solids/L in the test. 3,5-Dichlorophenole was used as reference item. The EC50 of the reference item was determined as 8.4 mg/L (95% confidence interval: 6.4 – 10.2 mg/L), which lies within the range of 5 – 30 mg/L. The 3 h EC50 was determined to be greater than 1000 mg/L and the 3 h NOEC was 1000 mg/L.
Reference
O2-Consumption
The values of the O2consumption (which is a measure for the viability of the bacteria) of controls, test and reference item are presented in the following table.
Table 1: O2consumption
No. |
Content |
Nominal Concentration (mg/L) |
O2consumption in mg/(L*min.) |
Mean O2cons. in mg/(L*min.) |
Mean O2cons. in mg/(L*h) |
1 |
Control |
0 |
0.9702 |
0.9292 |
55.7534 |
2 |
Control |
0 |
0.8883 |
||
3 |
Reference |
2.5 |
0.7419 |
0.6991 |
41.9471 |
4 |
Reference |
2.5 |
0.6563 |
||
5 |
Reference |
5 |
0.5867 |
0.6125 |
36.7471 |
6 |
Reference |
5 |
0.6383 |
||
7 |
Reference |
10 |
0.4191 |
0.4363 |
26.1800 |
8 |
Reference |
10 |
0.4536 |
||
9 |
Reference |
20 |
0.3127 |
0.3257 |
19.5416 |
10 |
Reference |
20 |
0.3386 |
||
11 |
Reference |
40 |
0.1634 |
0.1599 |
9.5416 |
12 |
Reference |
40 |
0.1556 |
||
13 |
Control |
0 |
0.7520 |
0.7271 |
43.6274 |
14 |
Control |
0 |
0.7022 |
||
15 |
Test Item |
1000 |
1.0636 |
1.2185 |
73.1071 |
16 |
Test Item |
1000 |
1.3733 |
||
17 |
Control |
0 |
1.0116 |
0.9983 |
59.8994 |
18 |
Control |
0 |
0.9850 |
The Control vessels no 13 and 14 were stated as outliers and were not used for the determination of the results, since the O2-consumption was much lower than in the other control vessels.
Inhibition
Inhibition of the O2consumption was calculated by comparison with the controls and is given in the following table.
Inhibition %
Item |
Nominal Concentration (mg/L) |
Inhibition in % |
Test Item |
1000 |
-26.4 |
Reference |
2.5 |
27,5 |
Reference |
5 |
36,5 |
Reference |
10 |
54,7 |
Reference |
20 |
66,2 |
Reference |
40 |
83,4 |
Description of key information
Aldehyde L was assessed in a activated sludge study according to EU-method C.11 and OECD guideline 209. A pre-test and a main-study were performed. The 3 h EC50 was determined to be greater than 1000 mg/L and the 3 h NOEC was 1000 mg/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
This study was performed in order to evaluate the effect of the test item on the respiration of activated sludge obtained from domestic sewage treatment plant. The activated sludge respiration test is a rapid screening method to identify substances which may adversely affect aerobic microorganisms. In the pre-test, the test item was tested using four concentrations (ranging from 1000 to 1 mg/L). Duration of the test was three hours. Activated sludge was used as inoculum. It was taken from a domestic sewage treatment plant and washed before usage. The dry matter was determined as 4.0 g suspended solids/L, giving a concentration of 1.6 g suspended solids/L in the test. As the pre-test was performed for range-finding only, no reference item was tested. No inhibition of the respiration of the activated sludge was detected in the treatments. The main study was performed as a limit-test with the nominal concentration of 1000 mg/L. Conduct of the study was the same as in the pre-test. The dry matter of the activated sludge was determined as 4.1 g suspended solids/L, giving a concentration of 1.64 g suspended solids/L in the test. 3,5-Dichlorophenole was used as reference item. The EC50 of the reference item was determined as 8.4 mg/L (95% confidence interval: 6.4 – 10.2 mg/L), which lies within the range of 5 – 30 mg/L. The 3 h EC50 was determined to be greater than 1000 mg/L and the 3 h NOEC was 1000 mg/L.
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