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Diss Factsheets
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EC number: 468-740-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
- Endpoint:
- specific investigations: other studies
- Adequacy of study:
- other information
Data source
Reference
- Reference Type:
- other: Body responsible for the test
- Title:
- Unnamed
Materials and methods
- Principles of method if other than guideline:
- Test procedure:
Vehicle: dimethyl formamide
Species: Mouse, CBA strain, inbred, SPF-Quality.
Source: Charles River France, L'Arbresle Cedex, France.
Number of animals: 20 females (four groups of five females
each group). (nulliparous and non-pregnant).
In the main study, three groups of five experimental animals
were epidermally exposed to 10%, 25% or 50% concentrations
of test substance in vehicle on three consecutive days. Five
vehicle control animals were similarly treated, but with
vehicle alone (Dimethyl formamide).
Three days after the last exposure, all animals were
injected with 3H-methyl thymidine and after five hours the
draining (auricular) lymph nodes were excised.
After precipitating the DNA of the lymph node cells,
radioactivity measurements were performed. The activity was
expressed as the number of Disintegrations Per Minute (DPM)
and a stimulation index is calculated for each group.
Results and discussion
- Details on results:
- The irritation of the ears as shown by the animals, was
considered not to have a toxicologically significant effect
on the activity of the nodes.
The majority of nodes were considered normal in size, except
for the nodes of one animal treated at 50% test substance
concentration, which were increased in size.
No macroscopic abnormalities of the surrounding area were
noted.
Mean DPM/animal values for the experimental groups treated
with test substance concentrations 10, 25 and 50% were 267,
888 and 1175 respectively.
The mean DPM/animal value for the vehicle control group was
242.
The SI values calculated for the substance concentrations
10, 25 and 50% were 1.1, 3.7 and 4.9 respectively.
These results indicate that the test substance could elicit
an SI >= 3. The data showed a dose-response and an EC3 value
of 21% was calculated.
The six monthly reliability check with Hexylcinnamic
aldehyde indicates that the Local Lymph Node Assay as
performed at NOTOX is an appropriate model for testing for
contact hypersensitivity.
Based on these results:
- according to the recommendations made in the test
guidelines, MTDID 3285 would be regarded as skin sensitizer.
- according to the Globally Harmonized System of
Classification and Labeling of Chemicals (GHS) of the United
Nations (New York and Geneva, 2003), MTDID 3285 should be
classified as skin sensitizer (Category 1).
- according to the EC criteria for classification and
labeling requirements for dangerous substances and
preparations (Council Directive 67/548/EEC), MTDID 3285
should be labeled as: may cause sensitization by skin
contact (R 43).
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.