Distilled acetalization product between glucose and C12, C14, C18, C20 ,C22 alcohol

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999-08-18 to 1999-09-17

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

yes

Remarks:

The deviations were not considered to have affected the outcome or the objectivatives of the study.

GLP compliance:

yes (incl. QA statement)

Remarks:

1998-05-28

Test material

Test material information

Constituent 1

Reference substance name:

Montanov L

IUPAC Name:

Montanov L

Details on test material:

Batch number: 1556 MP
Expiry date: july 2001
Appearence: white flakes
Packaging: White plastic flask
Quantity received and date: Approximately 160 g, received on july 12th, 1999
Storage: In darkness and at room temperature
Identificatyion number for the study: 99062

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Organisms, strains:
a mixture of different strains of micro-organisms found in activated sludge.
Justification of the choice:
required by international standards
Source:
activated sludge from a municipal sewage treatment plant receiving little or no industrial effluent (from Saint-Victor sur Loire -F-42-Loire)

Duration of test (contact time):

ca. 28 d

Initial test substance concentration

Initial conc.:

ca. 19.87 mg/L

Based on:

act. ingr.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

Preparation of the test substance:
no stock solution was prpared; the appropriate amounts of test substance were weighed, dispersed in 3 mL of demineralized water by heating at +75°C with stirring, and then added into the respective test medium and dispersed by magnetic stirring, to give the appropritae yest substance concentration into each replicate.
each test medium (without yest substance or reference substance had been areated by CO2-free air bubbling for a period of 15 hours before the start of the test.
The pH of test media was recorded.

Experimental conditions
Temperature of the test room: not recoded
Duration of the test: maintained during 28 days.
Test volume: 3,000 mL which was aerated with CO2-free air, at the rate of 1 to 2 bubbles per second 'in compliance with the rate preconised in the norm: 30-100 mL/minute).
pH of control and test media: recoded
containers: 5 litre carboys (clear glass)
The test was conduced in darkness (exept for the measuements performed in diffuse light)
Stirring: magnetic stirring was maintained in test media during the test.
Identification containers: colour-coded lagels showing the test substance code number, the replicate number and the starting date of the test (one label per container).

Eperimental system
The 3 assays involved were:
1 assay with the test substance at a nominal concentration corresponding to 15,00 mg TOC per litre , i.e. 19.87 mg test substance per litre (2 test flasks)
1 reference assay at a nominal concentration corresponding to 12,355 mg TOC per litre, i.e. 70 mg refernce substance per ltre (1 reference flask)
1 control assay with the absence of any substance ( 2 control flasks)

Measurements and experimental analyses
Determination of CO2 production by back titration for each assay avery two to three days for the first 10 days, and then every three to four days until the end of the test (28 days); the titration of residual Ba(OH)2 was performed using HCl 0.05N with phenolphtalein as a colorimetric indicator.
Measurements of pH were performed in control, reference, toxicity control and test media at the start and the end of the test.

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

Test performance:

The test substance is readily biodegradable in our experimental conditions.

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

ca. 81

Sampling time:

28 d

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

ca. 65

Sampling time:

13 d

Remarks on result:

other: (10 day-window)

BOD5 / COD results

Results with reference substance:

Biodegradation of the reference substance: 100% in 14 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test substance is readily biodegradable in our experimental conditions.

Executive summary:

Test objective

to evaluate the "ready" biodegradability of aqueous solutions of non-volatile organic test substance in the presence of micro-organisms under aerobic conditions.

Principle

method applicable to non volatile yest substance which may have low water solubility.

addition of a concentration of test substance correponding to a T.O.C. concentration included between 10 and 20 mg T.O.C. per litre of a defined test medium, inoculated with micro-organisms obtained from activated sludge.

Analytical monitoring over a 28 -day period of release of CO2 incorporated as BaCO3.

A control assay, i.e. without any test substance, was performed in the same conditions.

Evaluation and calculation of the biodegradation of the test substance comparing the total mean amount of CO2 produced by the test media (corrected for that derived from the control media), with the total amount of CO2 that the test substance could have theoretically produced (based on the carbon content of the test substance).

An assay to check the operation of the test system, using a refernce substance (sodium acetate) was perfomed during a 21 -day period.

Results for the test substance

%Degr.	St. dev.	Parameter	Sampling time	Remarks
ca. 81		CO2 evolution	28 d
ca. 65		CO2 evolution	13 d	(10 day-window)

Conclusion

The test substance is readily biodegradable in our experimental conditions.