6-amino-5-formamido-1,3-dimethyluracil

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study. Scientifically acceptable.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard test guideline, DIN 38 412 L15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

6-amino-5-formamido-1,3-dimethyluracil

EC Number:

231-501-4

EC Name:

6-amino-5-formamido-1,3-dimethyluracil

Cas Number:

7597-60-6

Molecular formula:

C7H10N4O3

IUPAC Name:

N-(6-amino-1,3-dimethyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)formamide

Details on test material:

- Name of test material (as cited in study report): 1,3-Dimethyl-4-amino-5-formylaminouracil
- Lot/batch No.: 36 087
- Analytical purity: 98.3 %
water solubility: 13 g/l, according to sponsor.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

The test solution was prepared by adding the test item to the test media without any pre-treatment. Then, the fish were placed into the test aquaria.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Source: Fischzucht Paul Eggers, 2354 Hohenweststedt, Germany
- Length at study initiation (length definition, mean, range and SD): 6.7 cm (range 5.7 - 7.2 cm)
- Weight at study initiation (mean and range, SD): 2.9 g (range 1.5 - 4.4 g)
- Housing and adaption Culture conditions: oil-free aerated and charcoal filtered tap water, flow-through system; Water temperature: 20 +/- 1 °C Duration of housing: about 1 month Mortality during the time of housing: 1.4 % Mortality, Medical treatment: twice with 0.05 mg/l malachite green chloride, once with 10 mg/l tetracycline hydrochloride
- Feeding during test: 1 day before and during exposure.



ACCLIMATION
- Acclimation period: 3 days
- Type and amount of food: Growing feed F/B 50, ad libitum.
- Feeding frequency: daily
- Health during acclimation (any mortality observed): no mortality observed

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/l

Test temperature:

21 °C

pH:

7.3 - 7.8

Dissolved oxygen:

7.2 - 8.8 mg/l

Nominal and measured concentrations:

5000 and 10000 mg/l (based on the results of a range-finding test). In addition, a control was tested in parallel.

Details on test conditions:

TEST SYSTEM
- Test vessel: All glass aquaria (30 x 22 x 24 cm)
- Type (delete if not applicable): open / closed
- Fill volume: 10 l
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 2.9 g fish/l test water


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, Part II, 1982; prepared from fully demineralised tap water (conductivity: max 10 micro MHO) by adding: 294.0 mg/L CaCl2 * 2 H2O, 123.3 mg/L MgSO4 + 7 H2O, 63.0 mg/L NaHCO3, 5.5 mg/L KCl
- Acid capacity: 0.8 mmol/l:
- Ratio Na/K ions: 10 : 1
- Ca/mg ratio:4 : 1


OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 1, 4, 24, 48, 72 and 96 h.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

5 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

5 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

48 h-LC50 of the positive control Chloroacetamide: about 32 mg/l (corresponds to normal sensitivity).

Reported statistics and error estimates:

The NOEC, the LC0, the LC50 and the LC100 were determined directly from the raw data.

Any other information on results incl. tables

Sublethal observations / clinical signs:

During the 96 h exposure to 1,3-dimethyl-4-amino-5-formylaminouracil, mortalities were as follows:

Nominal test Cumulative mortalitiy (%) after x hours
concentration 1 / 4 / 24 / 48 / 72 / 96
(mg/l)
Control 0 / 0 / 0 / 0 / 0 / 0
5000 0 / 0 / 0 / 0 / 0 / 0
10000 0 / 0 / 0 / 1 / 2 / 2

Symptoms of intoxication:
No symptoms of intoxications were observed during the 96 hours of expsoure in the control and at any test concentration tested.