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EC number: 700-757-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 3 December 2001 - 28 January 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1224866-76-5
- Cas Number:
- 1224866-76-5
- IUPAC Name:
- 1224866-76-5
- Details on test material:
- - Name of test material (as cited in study report): Glypho
- Substance type: pure active substance
- Physical state: liquid
- Stability under test conditions: Stable
- Storage condition of test material: Refrigeration at ca. 4°C
- Other: bottles not to be filled > 90%, oxygen inhibits polymerisation
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): ARA Ergolz II, Füllinsdorf, Switzerland
- Preparation of inoculum for exposure: The sludge was washed once with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenized aliquot of the final sludge suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated. Based on this ratio, wet sludge were suspended in test water to obtain a concentration equivalent to 4 g (±10%) dry material per liter. During the holding period before use, the sludge was aerated at room temperature. Prior to use, the sludge was diluted with test water to a concentration of 1 g per liter (dry weight basis). This diluted activated sludge was added to test water to obtain a final concentration of 30 mg dry material per liter.
- Pretreatment: None
- Concentration of sludge: 30 mg/L dry weight basis in reactor
- Initial cell/biomass concentration: Not reported
- Water filtered: No - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Stock solution 1
KH2PO4 8.50 g/L
K2HPO4 21.75 g/L
Na2HPO4 ∙ 2H2O 33.40 g/L
NH4Cl 0.50 g/L
Stock solution 2
MgSO4 ∙ 7H2O 22.50 g/L
Stock solution 3
CaCl2 ∙ 2H2O 36.40 g/L
Stock solution 4
FeCl3 ∙ 6H2O 0.25 g/L
10 mL of stock solution No. 1 and 1 mL each of stock solution Nos. 2, 3 and 4 were combined and made up to 1000 mL with purified water. The pH was adjusted from 7.7 to 7.4 with a diluted hydrochloric acid solution. This mixture was used for all dilutions and preparation of activated sludge.
- Additional substrate: None
- Solubilising agent (type and concentration if used): None
- Test temperature: 22 °C
- pH: 7.2 - 7.4 at test initiation.
- pH adjusted: No
- Aeration of dilution water: Sealed bottles stirred in a SAPROMAT D12 (Voith GmbH, Heidenheim,
Germany)
- Suspended solids concentration: 30 mg/L sludge solids dry weight
- Continuous darkness: Yes
TEST SYSTEM
- Culturing apparatus: SAPROMAT D12 (Voith GmbH, Heidenheim, Germany), 500 mL reactor volume, 250 mL solution per reactor.
- Number of culture flasks/concentration: Two
- Method used to create aerobic conditions: Continuous stirring. Consumed oxygen replaced by electrolytic generation of a copper sulfate solution.
- Measuring equipment: SAPROMAT D12 (Voith GmbH, Heidenheim, Germany)
- Details of trap for CO2 and volatile organics if used: Soda lime, used to trap CO2
SAMPLING
- Sampling frequency: Once daily on working days, non-destructive testing
- Sampling method: Electrode-type manometer.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes, in duplicate
- Abiotic sterile control: Yes, single flask inhibited with mercury dichloride
- Toxicity control: Yes, single flask
CONCENTRATIONS:
- Test substance flasks: 100 mg/L
- Reference substance flasks: 100 mg/L
- Abiotic control: 100 mg/L test substance, 10 mg/L HgCl2
- Toxicity control: 100 mg/L test substance plus 100 mg/L reference substance
- The test item was first weighed onto glass slides. The glass slides were then transferred to the designated test flasks and test water was added. No emulsifiers or solvents were used but ultrasound dispersion was employed for five minutes to obtain a suspension of the test item as homogeneous as possible. ThOD of test substance could not be calculated, but COD was measured according to the EU Commission Directive 92/69/EEC, C.6 following DIN 38414-S9. The COD determination was not GLP-compliant but was performed in compliance with DIN EN 45001 (General criteria for the operation of testing laboratories).
STATISTICAL METHODS:
BOD = [(mg O2 uptake/L by test and/or reference item) - (mg O2 uptake/L by inoculum control)] / [mg test and/or reference item per liter]
% biodegradation = [BOD (mg O2 /mg chemical)] / [COD or ThOD (mg O2 /mg chemical)]
where COD is used for test substance and ThOD is used for reference substance
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 82
- Sampling time:
- 28 d
- Remarks on result:
- other: Biodegradation >10% on day 7, >60% on day 17
BOD5 / COD results
- Results with reference substance:
- The percent biodegradation of the reference item sodium benzoate was calculated based on cumulative oxygen demand (Table 1) and the theoretical oxygen demand of 1.67 mg O2/mg. In the procedure controls, the reference item was degraded by an average of 85% by Exposure Day 14, thus confirming suitability of the activated sludge.
In the toxicity control, the course of biodegradation over the 28-day exposure period was intermediate between the reference substance and the test substance (Figure 1). Within 14 days of exposure, biodegradation amounted to 64%. According to the test guidelines, the test substance had no inhibitory effect on activated sludge microorganisms at a concentration of 100 mg/L because biodegradation in the toxicity control was >25% within 14 days.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- % difference in extreme values <20% at end of test, % degradation of reference compound > 60% on day 14.
- Interpretation of results:
- readily biodegradable
- Conclusions:
- CAS# 1224866-76-5 is readily biodegradable
- Executive summary:
CAS# 1224866-76-5 biodegradability was assessed in a respirometric assay conducted according to OECD301F. Because the test substance theoretical oxygen demand could not be calculated, chemical oxygen demand was measured and used to calculate percent biodegradation. Reference substance was sodium benzoate. Test substance biodegradation was >10% by day 7, >60% by day 17, and 82% on day 28. CAS# 1224866-76-5 is readily biodegradable.
The test was conducted in accordance with OECD guideline and compliant with GLP criteria. It is considered reliable without restrictions and is suitable for use in Risk Assessment, Classification & Labeling, and PBT Analysis.
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