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EC number: 203-581-0 | CAS number: 108-42-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: short-term
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: OECD guideline study with acceptable restrictions.
Data source
Reference
- Reference Type:
- publication
- Title:
- Phytotoxicity studies with Lactuca sativa in soil and nutrient solution
- Author:
- Hulzebos EM, Adema DMM, Dirven-van Breemen, Henzen L, van Dis, WA, Herbold, HA, Hoekstra JA, Baerselman, R, van Gestel CAM
- Year:
- 1 993
- Bibliographic source:
- Environ. Toxicol. Chem. 12 (6): 1079-1094
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
- Deviations:
- not specified
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 3-chloroaniline
- EC Number:
- 203-581-0
- EC Name:
- 3-chloroaniline
- Cas Number:
- 108-42-9
- Molecular formula:
- C6H6ClN
- IUPAC Name:
- 3-chloroaniline
- Test material form:
- other: liquid
- Details on test material:
- - Purity: ≥ 95 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Soil tests:
3-chloroaniline was dissolved in water, added to the soil, and thoroughly mixed with a household mixer. 3-chloroaniline has a low water solubility, also it was either dissolved in acetone and/or mixed with a small amount of quartz sand. The compound was mixed through the soil using the quartz sand as a carrier.
Nominal concentrations in micrograms per gram dry soil were spaced by a factor of 3.2. Duplicate trays were used for the control and for at least three tes concentrations. The 0.25 L plastic trays contained 400 g of soil at 25 to 30 % moisture content (80 % of the water-holding capacity). In each tray 10 seeds were sown. The trays were covered with glass plates until germination of seedings. After removal of the glass plates, exporation of water was compensated daily by adding demineralized water. Only the first five germinated seedings were required, and any additional germinated seedings were discarded. After 7 and 14 days, shoots were harvested by cutting them off at soil level: the fresh weight of each plant was determined immediately after harvesting. Water content and pH of the soli were checked at the start and the end of the experiment.
Test substrate
- Vehicle:
- yes
Test organisms
- Species:
- Lactuca sativa
- Plant group:
- other: no data
Study design
- Test type:
- seedling emergence toxicity test
- Study type:
- laboratory study
- Substrate type:
- natural soil
- Total exposure duration:
- 14 d
Test conditions
- Test temperature:
- 21 ± 4 °C
- pH:
- 7.5
- Moisture:
- 25 - 30 %
- Details on test conditions:
- - Test container: 0.25 L plastic trays
- Amount of soil: 400 g
- No. of seeds per container: 10
Nominal concentration (µg/g dry soil) were spaced by a factor of 3.2.
Duplicate trays were used for the control and for the least three test concentrations.
Soil was collected twice from an orchard.
- Photoperiod: 16 hour light/8 hour darkness, assuming an average wavelength of 400 to 700 nm;
- Light intensity: luminance of 350 ± 50 μE/m2/s, measured at the top of the canopy.
- Day/night temperatures: 25 ± 3 °C during the day, 20 ± 3 °C during the night;
- Relative humidity (%): 40 ± 5 % during light periods and 80 ± 5 % during dark periods; - Nominal and measured concentrations:
- When necessary a range-finding test could be performed to provide guidance on concentrations/rates to be tested in definitive dose-response study. For the range-finding test, the test concentrations/rates should be widely spaced (e.g. 0.1, 1.0, 10, 100 and 1000 mg/kg dry soil). For crop protection products concentrations/rates could be based on the recommended or maximum concentration or application rate, e.g. 1/100, 1/10, 1/1 of the recommended/maximum concentration or application rate.
Results and discussion
Effect concentrations
- Species:
- Lactuca sativa
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- 15 mg/kg soil ww
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks on result:
- other: (12 - 19 µg/g)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The acute toxcity of terrestrial plants ( Lactuca satvia) was determined during a period of 14 days. An EC50 of 15 µg/g soil was measured.
- Executive summary:
The acute toxcity of terrestrial plants ( Lactuca satvia) was determined during a period of 14 days. An EC50 of 15 mg/kg soil was measured.
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