2,6-Benzothiazolediamine, 4,5,6,7-tetrahydro-N6-propyl-, (6S)-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(S)-2-Amino-6-propylamino-4,5,6,7-tetrahydrobenzothiazole

Cas Number:

104632-26-0

Molecular formula:

C10 H17 N3 S

IUPAC Name:

(S)-2-Amino-6-propylamino-4,5,6,7-tetrahydrobenzothiazole

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

>= 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

>= 70 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Conclusions:

Two valid experiments were performed.
The first experiment was performed using eight concentrations ranging from 40 to 1000 mg/L. Twenty daphnia were exposed to the test item for
48 hours in a static test system.After 24 and 48 hours, the immobilized daphnia were counted.
Four concentrations ranging from 40 to 160 mg/L showed toxicity between 0 and 100% immobilization after 48 hours of exposure. 5% of the
animals were immobilized in the control (criterion: <= 10%).
As the lowest concentration in the first experiment showed 15% immobilization, a second experiment was performed using the two concentrations
16 and 25 mg/L. None of the daphnia were immobilized in the control and the treatments.
At the beginning and at the end of each experiment, the content of the test item in the test solutions was determined using HPLC. The recovery after
48 hours was in a range of 100 to 109 % of the start concentration. The measured concentrations ranged from 96 to 111% of the nominal
concentration. Therefore, the determination of the results was based on the nominal concentrations.
The following results could be determined for the test item Pramipexole (species: Daphnia magna).
24h NOEC = 40 mg/L
48h NOEC = 25 mg/L
24h EC50i = 200 mg/L
48h EC50i = 70 mg/L