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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Principles of method if other than guideline:
Method: BASF-Test: 50 µL of the test substance were applied to the conjunctival sac of one eye in 2 animals. The animals were observed after treatment and findings were recorded daily. The eyes were not washed out after 24 hours as specified in OECD Guideline 405. The report describes findings after 1 hour, and after 1, 2 and 8 days (end of the observation period).
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Butyryl chloride
EC Number:
205-498-5
EC Name:
Butyryl chloride
Cas Number:
141-75-3
Molecular formula:
C4H7ClO
IUPAC Name:
butanoyl chloride
Details on test material:
- Name of test material (as cited in study report): n-büttersäurechlorid
- Substance number: XXVI 622
- Substance type: liquid

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Weight at study initiation: male: 2.91 kg; female: 3.1 kg

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: the other eye was treated with a physiological salt solution
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 50 µL
Duration of treatment / exposure:
single application, no washing was performed
Observation period (in vivo):
1 hour, 24 hours, 48 hours and day 8 post-treatment
Number of animals or in vitro replicates:
2
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing (if done): no

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
iris score
Basis:
mean
Time point:
24/48 h
Score:
0
Max. score:
2
Reversibility:
other: no effects were observed
Remarks on result:
other: after 1 hour and after 8 days the score was 0; scar formation observed at all time points
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
24/48 h
Score:
3
Max. score:
4
Reversibility:
not reversible
Remarks on result:
other: after 1 hour the score was 3 and after 8 days the score was 4; scar formation at all time points
Irritation parameter:
chemosis score
Basis:
mean
Time point:
24/48 h
Score:
3.3
Max. score:
4
Reversibility:
not reversible
Remarks on result:
other: after 1 hour the score was 2 and after 8 days the score was 3; scar formation at all time points
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
24/48 h
Score:
2
Max. score:
3
Reversibility:
not reversible
Remarks on result:
other: after 1 hour the score was 1 and after 8 days the score was 2; scar formation at all time points
Irritant / corrosive response data:
The colour of the nictitating membrane and the mucous membrane was grey 1 hour post-treatment and white 24 and 48 hours and 8 days post-treatment. In one animal strong discharge was observed 8 days post-treatment and therefore scoring of the eyes was not possible.

Applicant's summary and conclusion

Interpretation of results:
corrosive
Remarks:
Migrated information